Ultra-LEAF™ Purified anti-Asialo-GM1 Antibody

Product Details

Verified Reactivity

Mouse, Rat

Antibody Type

Polyclonal

Host Species

Rabbit

Immunogen

Asialo-GM1

Formulation

Lyophilized powder.

Endotoxin Level

Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.

Preparation

Reconstitute with 1 ml sterile deionized, distilled, endotoxin-free water. Gamma globulin fraction of serum was precipitated using 50% ammonium sulfate followed by dialysis with phosphate buffered saline.

Concentration

Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.

Application

FC - Quality tested
Depletion - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. Optimal staining for flow cytometry may vary from lot-to-lot. This antibody has been reported to deplete NK cells in vivo. A usage of 50 µl per mouse is recommended, but optimal usage will vary depending on the strain of mouse and experimental conditions^2-4. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody recognizes asialo-GM1. It does not react with other glycolipids, such as GM1 and Asialo-GM2.

This antibody can partially block IL-12 induced IFN-γ production but does not affect other systemic action of IL-12. 

This product may contain other non-IgG subtypes.

Application References

(PubMed link indicates BioLegend citation)

1. Naiki M, et al. 1974. J. Immunol. 113:84.
2. Kasai M, et al. 1981. Nature. 291:334. (Depletion)
3. Nishikado H, et al. 2011. J. Immunol. 186:5766. (Depletion)
4. Zhou G, et al. 2013. Eur. J. Immunol. 43:929. (Depletion)

Product Citations

1. Wang X, et al. 2022. Viruses. 14:. PubMed
2. Hu J, et al. 2015. J Virol. 89: 10383 - 10398. PubMed
3. Böttcher JP, et al. 2018. Cell. 172:1022. PubMed
4. Li P, et al. 2020. Nat Commun. 3.504861111. PubMed
5. André P et al. 2018. Cell. 175(7):1731-1743 . PubMed
6. Lin Q, et al. 2021. Nat Commun. 12:2537. PubMed
7. Shannon JP, et al. 2021. Immunity. 54(2):276-290.e5. PubMed
8. Ren Y, et al. 2015. Sci Rep. 5: 18099. PubMed
9. Turaj AH et al. 2017. Cancer cell. 32(6):777-791 . PubMed
10. Dai HS et al. 2017. Immunity. 47(1):159-170 . PubMed
11. Pelly VS, et al. 2021. Cancer Discov. 11:2602. PubMed
12. Gauthier L, et al. 2019. Cell. 177:1701. PubMed
13. Bonavita E, et al. 2020. Immunity. 1215:53. PubMed
14. Dubrot J, et al. 2021. Immunity. 54(3):571-585.e6. PubMed
15. Ma Q, et al. 2021. Precis Clin Med. 4:155. PubMed
16. Manangeeswaran M, et al. 2020. Front Immunol. 11:237. PubMed

RRID

SCR_001134 (BioLegend Cat. No. 146002)

Antigen Details

Structure

Asialo-GM1 is a GM1 derivative without a sialic acid group, with the structure: βDGalp(1-3)βDGalpNAc(1-4)βDGalp(1-4)βDGlcp(1-1)Cer

Distribution

NK cells, basophils, splenic T lymphocytes, highest concentrations on neurons

Function

Supports cellular identification, neuronal plasticity, and repair mechanisms

Interaction

Facilitates cellular recognition and cell-to-cell communication

Ligand/Receptor

Ligand for cholera toxin

Cell Type

Basophils, Neurons, NK cells, T cells

Biology Area

Cell Biology, Immunology, Innate Immunity, Signal Transduction

Antigen References

1. Stein-Douglas K, et al. 1979. J. Exp. Med. 143:822. 
2. Kasai M, et al. 1980. Eur. J. Immunol. 10:175. 
3. Young WW Jr, et al. 1980. J. Immunol. 124:199. 
4. Bansal AS, et al. 1994. J. Clin. Path. 47:300.

Gene ID

NA

UniProt

View information about Asialo-GM1 on UniProt.org

Documentation

• Technical Data Sheet (pdf)
• Certificate of Analysis
• Safety Data Sheet

Reviews

Related Pages & Pathways

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Other Formats

Certificate of Analysis