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Ultra-LEAF™ Purified anti-human β-NGF Antibody

Pricing & Availability
Clone
A16145B (See other available formats)
Regulatory Status
RUO
Other Names
Beta-Nerve growth factor, Nerve Growth Factor, NGFB
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
A16145B_ULEAF_B-NGF_Antibody_1_082619_updated.png
Recombinant human β-NGF (Black Circles, Cat. No. 762206) induces the proliferation of human erythroleukemia TF-1 cells. Anti-human β-NGF antibody (clone A16145B , Purple Squares) neutralizes the proliferation function of recombinant human β-NGF at 0.08 µg/mL in TF-1 cells in a dose-dependent manner while the isotype control (Cat. No. 400264, Green Triangles) does not have the effect. The ND50 = 0.1-0.8 µg/mL.
  • A16145B_ULEAF_B-NGF_Antibody_1_082619_updated.png
    Recombinant human β-NGF (Black Circles, Cat. No. 762206) induces the proliferation of human erythroleukemia TF-1 cells. Anti-human β-NGF antibody (clone A16145B , Purple Squares) neutralizes the proliferation function of recombinant human β-NGF at 0.08 µg/mL in TF-1 cells in a dose-dependent manner while the isotype control (Cat. No. 400264, Green Triangles) does not have the effect. The ND50 = 0.1-0.8 µg/mL.
  • A16145B_ULEAF_B-NGF_Antibody_2_082619_updated.png
    Recombinant Mouse β-NGF (Black Circles, Cat. No. 598104) induces the proliferation of human erythroleukemia TF-1 cells. Anti-human β-NGF antibody (clone A16145B , Purple Squares) neutralizes the proliferation function of recombinant mouse β-NGF at 0.005 µg/mL in TF-1 cells in a dose-dependent manner while the isotype control (Cat. No. 400264, Green Triangles) does not have the effect. The ND50 = 5-20 µg/mL.
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617903 100 µg 270 CHF
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617904 1 mg 715 CHF
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Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.

Description

Neurotrophins are growth factors that modulate growth, differentiation, and survival of neurons. These structurally related proteins include nerve growth factor (NGF/β-NGF), brain-derived neurogenic factor (BDNF), neurotrophin-3, and neurotrophin-4/5. They are highly homologous and share a similar structure of a tertiary folded, cysteine rich "knot". Neurotrophins are critical to the development of the central and peripheral nervous systems, and they play important roles in injury-induced neuronal regeneration. β-NGF is a member of the neurotrophin family which controls growth, survival, and maturation of neurons. In peripheral and central neurons, neurotrophins are important regulators for survival, differentiation, and maintenance of nerve cells. They are detectable throughout embryogenesis and into the adult stage in the central and peripheral nervous systems. In animal models, β-NGF prevents neuronal degeneration and has become a therapeutical target in neuron regeneration studies. β-NGF is also critical for the survival and maintenance of sympathetic and sensory neurons. It is the main neurotrophin that maintains homeostasis of neurogenesis. It prevents neurons from undergoing apoptosis and promotes maturation while supporting axonal branching. Two receptors are known to interact with NGF, p75NTR and TrkA. The NGF-TrkA signaling pathway induces two pathways through activation of PI3K-Akt or Ras-MAPK. Either one of the pathways leads to CREB phosphorylation and increases expression of anti-apoptosis genes to keep neurons alive. Animal models of neurodegenerative diseases suggest that NGF can promote myelin repair of neurons in the peripheral nervous system. Clinical studies indicate that dysregulation of NGF signaling is related to Alzheimer's disease.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant Human β-NGF
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level
Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

Neut - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by neutralization of the proliferation induced by recombinant human β-NGF (0.08 µg/mL) on TF-1 human erythroleukemia cells. ND50 range: 0.1 - 0.8 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

Product Citations
  1. Wu BW, et al. 2022. Viruses. 14:. PubMed
RRID
AB_2814462 (BioLegend Cat. No. 617903)
AB_2814462 (BioLegend Cat. No. 617904)

Antigen Details

Structure
Dimer
Distribution

Hypothalamus, pituitary, thyroid gland, testes, epididymis, vascular smooth muscle cells, fibroblasts, myofibroblasts, mast cells, eosinophils.

Function
β-NGF is important for maintaining neuron homeostasis.
Interaction
Neurons, astrocytes, eosinophils, B, T, and mast cells.
Ligand/Receptor
Low-affinity NGF receptor (p75NTR); high-affinity NGF receptor (TrkA).
Cell Type
B cells, Eosinophils, Fibroblasts, Mast cells, Mature Neurons, Neural Stem Cells, Neurons, T cells
Biology Area
Cell Biology, Cell Proliferation and Viability, Neurodegeneration, Neuroscience, Synaptic Biology
Molecular Family
Cytokines/Chemokines, Growth Factors
Antigen References
  1. Ullrich A, et al. 1983. Nature. 303:821.
  2. Klein R, et al. 1991. Cell. 65:189.
  3. Welker P, et al. 2000. Immunology 99:418.
  4. Fahnestock M, et al. 2004. Prog. Brain Res. 146:101.
  5. Bruno MA. Cuello AC. 2006. Proc. Natl. Acad. Sci. USA 103:6735.
  6. Aloe L, et al. 2015. Curr. Neuropharmacol. 13:294.
Gene ID
4803 View all products for this Gene ID
UniProt
View information about beta-NGF on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 08.14.2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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