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Ultra-LEAF™ Purified anti-human CCL22 Antibody

Pricing & Availability
Clone
A15083D (See other available formats)
Regulatory Status
RUO
Other Names
Macrophage derived cytokine (MDC), Small inducible cytokine subfamily A, member 22 (Scya22), stimulated T cell chemotactic protein (STCP-1)
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
A15083D_ULEAF_CCL22_NEUT_Antibody_020717
Recombinant human CCL22 chemoattracts Baf3-hCCR4 transfectants in a dose dependent manner (black squares). Anti-human CCL22 monoclonal antibody (purple circles) neutralizes the chemoattraction of Baf3-hCCR4 transfectants induced by recombinant human CCL22 (1.2 ng/ml). The ND50 = 0.4 - 2.4 µg/ml.
  • A15083D_ULEAF_CCL22_NEUT_Antibody_020717
    Recombinant human CCL22 chemoattracts Baf3-hCCR4 transfectants in a dose dependent manner (black squares). Anti-human CCL22 monoclonal antibody (purple circles) neutralizes the chemoattraction of Baf3-hCCR4 transfectants induced by recombinant human CCL22 (1.2 ng/ml). The ND50 = 0.4 - 2.4 µg/ml.
  • A15083D_ULEAF_CCL22_NEUT_Antibody_2_062524
    Enriched human peripheral blood monocytes were cultured (3 days) with recombinant human GM-CSF. 1X Monensin (Cat. No. 420701) was added for the last 4 hours of culture. Cells were then fixed and permeabilized with Cyto-Fast™ Fix/Perm Buffer (Cat. 426803) and then intracellularly stained with Ultra-LEAF™ Purified anti-human CCL22 (clone A15083D) (left) or purified mouse IgG2a, κ isotype control (right) followed by anti-mouse IgG PE, then co-stained with anti-human CD206 (clone 15-2) APC.
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694403 100 µg 270 CHF
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694404 1 mg 715 CHF
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Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.

Description

CCL22 is thymus-specific chemokine engaged in the recruitment of T cells. CCL22 is associated with different diseases such as allergen-induced lung inflammation, atopic dermatitis, and lymphoma. Also, high levels of CCL22 have been detected in the CNS in multiple sclerosis (MS) and experimental autoimmune encephalomyelitis (EAE). In addition, CCR4+ T cells have been linked to endotoxic shock, rheumatoid arthritis, T cell lymphoma, and autoimmune diabetes. CCL22 is a prostaglandin-dependent pyrogen, acting in the anterior hypothalamus to induce hyperthermia via activation of brown adipose tissue.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human CCL22
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level
Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

Neut - Quality tested
ICFC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by neutralizing activity. The ND50 is 1.5 - 4.5 µg/mL. For intracellular flow cytometry using our Cyto-Fast™ Fix/Perm Buffer Set, the suggested use of this reagent is ≤ 0.06 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes
Clones A15083A and A15083D do not block each other.

Clone A15083A does not block the reference clone T51-719 (BD Bioscience).

Clones A15083A and A15083D showed good staining in both Cyto-Fast™ Fix/Perm Buffer Set and True-Phos™ Perm Buffer.
RRID
AB_2650730 (BioLegend Cat. No. 694403)
AB_2650730 (BioLegend Cat. No. 694404)

Antigen Details

Structure
Chemokine
Distribution

Macrophages, NK cells, IFN-γ stimulated human keratinocytes, thymic epithelial cells, and intestinal epithelial cells

Function
CCL22 induces the migration of CCR4+ Th2 cells, Tregs, DC, and NK cells. It is induced by LPS and CD40 antibody in macrophages. It is upregulated by Th2 cytokines (IL-4 and IL-5) and downregulated by Th1 cytokines (IFN-γ).
Interaction
Dendritic cells, NK cells, Th2 cells, T regs, CLA+ skin-homing memory/effector T cells
Ligand/Receptor
CCR4
Biology Area
Cell Biology, Signal Transduction
Molecular Family
Cytokines/Chemokines
Antigen References
  1. Godiska R, et al. 1997. J Exp Med. 185:1595-604.
  2. Imai T, et al. 1998. J Biol Chem. 273:16551-4.
  3. Yamashita U and Kuroda E. 2002. Crit Rev Immunol. 22:105-14.
  4. Horikawa T, et al. 2002. Int Immunol. 14:767-73.
  5. Mailloux AW and Young MR. 2009. J Immunol. 182:2753-65.
  6. Dogan RN, et al. 2011. J Leukoc Biol. 89:93-104.
  7. Osborn O, et al. 2011. Cytokine. 53:311-9.
Gene ID
6367 View all products for this Gene ID
UniProt
View information about CCL22 on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 4    Revision Date: 06.25.2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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