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Ultra-LEAF™ Purified anti-human CD112 (Nectin-2) Antibody

Pricing & Availability
Clone
TX31 (See other available formats)
Regulatory Status
RUO
Other Names
Poliovirus Receptor Related 2 Protein (PRR2), Nectin-2, Hve B
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
1-TX31_Pure_110308
KG1a (human myeloid leukemia cell line) stained with purified TX31, followed by biotinylated anti-mouse IgG and Sav-PE
  • 1-TX31_Pure_110308
    KG1a (human myeloid leukemia cell line) stained with purified TX31, followed by biotinylated anti-mouse IgG and Sav-PE
  • 2-TX31_ULEAF_CD112_Antibody_100119
    Recombinant human CD112R (Black Circles, Cat. No. 772106) binds to immobilized recombinant human CD112. Anti-human CD112 antibody (Purple Squares, Clone TX31) inhibits the binding in a dose-dependent manner whereas the isotype control (Green Triangles, Cat. No. 400193) does not have this effect. This antibody blocks the binding of 15 µg/mL recombinant human CD112R protein to 1 µg/mL of immobilized recombinant human CD112 with an ND50 = 0.8 - 5.0 μg/mL.
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337423 100 µg 270 CHF
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337424 1 mg 715 CHF
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Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.

Description

CD112, known as poliovirus receptor related 2 protein (PRR2), Nectin-2, or Hve B, is a type I transmembrane glycoprotein, a member of the Ig gene superfamily. It is primarily found on myelomonocytic cells, megakaryocytes, dendritic cells, mast cells, CD34 positive stem cells, endothelial cells, epithelial cells, and neuronal cells. CD112 functions as a receptor for α-herpes viruses HSV-1 and pseudorabies virus (PRV). CD112 is an adhesion molecule involved in the formation of cell junction and regulation of NK- and T cell-mediated cytotoxicity through interaction with CD226 (DNAM-1) and other nectin family molecules.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level
Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

FC - Quality tested
Block - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µl volume. The antibody also blocks the binding between recombinant human CD112/Nectin-2 and recombinant human CD112R (Cat. No. 772106). The ND50 range is 0.8 - 5.0 μg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications include: partially block CD226-Fc binding to CD112 transfectant cells and partially inhibit the cytotoxicity mediated by NK and T cells.

Application References

(PubMed link indicates BioLegend citation)
  1. Tahara-Hanaoka S, et al.. 2004. Int. Immunol. 16:533
  2. Lim O, et al. 2013. PLoS One. 8:e53611. PubMed
  3. Gillory LA, 2013. PLoS One. 8:77753. PubMed
RRID
AB_2819965 (BioLegend Cat. No. 337423)
AB_2819965 (BioLegend Cat. No. 337424)

Antigen Details

Structure
Type I transmembrane glycoprotein, Ig superfamily
Distribution

Myelomonocytic cells, megakaryocytes, dendritic cells, mast cells, CD34-positive stem cells, endothelial cells, epithelial cells, and neuronal cells.

Function
Adhesion, receptor for some Herpes Viruses
Ligand/Receptor
CD226, CD155, Nectin-1, -3, and -4, Herpes Viruses
Cell Type
Dendritic cells, Endothelial cells, Epithelial cells, Hematopoietic stem and progenitors, Mast cells, Megakaryocytes
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroscience, Synaptic Biology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Zola H et al. Eds. 2007. Leukocyte and Stromal Cell Molecules:The CD Markers. Wiely-Liss A John Wiley & Sons Inc, Publication
2. Bachelet I, et al. 2006. J. Bio. Chem. 281:27190
3. Pende D, et al. 2006. Blood 107:2030
4. Bottino C, et al. 2003. J. Exp. Med. 198:557

Gene ID
5819 View all products for this Gene ID
UniProt
View information about CD112 on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 10.01.2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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