- Clone
- A12 (7D4) (See other available formats)
- Regulatory Status
- RUO
- Other Names
- SLAM, Signaling Lymphocyte Activation Molecule, IPO-3
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
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306317 | 100 µg | 270 CHF | ||||
306318 | 1 mg | 743 CHF |
Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.
CD150 is a 70-95 kD type I transmembrane glycoprotein also known as SLAM or IPO-3. It is a member of the Ig superfamily. It is expressed on a subset of T cells, B cells, dendritic cells, and endothelial cells. The expression of CD150 is upregulated upon activation. CD150 binds to itself as the ligand to be involved in B cell costimulation, proliferation, immunoglobulin production, and signal transduction.
Product DetailsProduct Details
- Verified Reactivity
- Human, Cynomolgus, Rhesus
- Reported Reactivity
- African Green, Baboon
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Activated human PBMC
- Formulation
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
- Endotoxin Level
- Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
- Preparation
- The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections, immunoprecipitation4, and costimulation1,5 of IFN-gamma production and T cell proliferation. The Ultra-LEAF™ purified antibody (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 306317 and 306318).
-
Application References
(PubMed link indicates BioLegend citation) -
- Garcia V, et al. 2001. J. Immunol. 167:5719. (Costim)
- Vincent S, et al. 2002. J. Virol. 76:6121.
- Cocks B, et al. 1995. Nature 376:260.
- Sayos J, et al. 2001. Blood 97:3867. (IP)
- Aversa G, et al. 1997. J. Immunol. 158:4036. (Costim)
- Spencer M, et al. 2010. Am. J. Physiol Endocrinol Metab. 299:1016. PubMed
- RRID
-
AB_2860817 (BioLegend Cat. No. 306317)
AB_2860817 (BioLegend Cat. No. 306318)
Antigen Details
- Structure
- Ig superfamily, type I transmembrane glycoprotein, 70-95 kD
- Distribution
-
T subset (upregulated after activation), B cells, dendritic cells, endothelial cells
- Function
- B cell costimulation, proliferation and Ig production
- Ligand/Receptor
- CD150 (self ligand)
- Cell Type
- B cells, Dendritic cells, Endothelial cells, T cells, Tregs
- Biology Area
- Costimulatory Molecules, Immunology, Innate Immunity
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
-
1. Cocks B, et al. 1995. Nature 376:260.
2. Pinchouk V, et al. 1988. AntiCancer Res. 8:1377.
3. Polacino P, et al. 1996. J. Med. Primatol. 25:201.
4. Punnonen J, et al. 1997. J. Exp. Med. 185:993. - Gene ID
- 6504 View all products for this Gene ID
- UniProt
- View information about CD150 on UniProt.org
Related FAQs
- Do you guarantee that your antibodies are totally pathogen free?
-
BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
- Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
-
No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
- Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
-
No, BioLegend does not test for pathogens in-house unless otherwise indicated. However, we can recommend an outside vendor to perform this testing as needed.
- Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
-
We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.
Other Formats
View All CD150 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
FITC anti-human CD150 (SLAM) | A12 (7D4) | FC |
PE anti-human CD150 (SLAM) | A12 (7D4) | FC |
Purified anti-human CD150 (SLAM) | A12 (7D4) | FC,IHC-F,Costim,IP |
Alexa Fluor® 488 anti-human CD150 (SLAM) | A12 (7D4) | FC |
TotalSeq™-A0870 anti-human CD150 (SLAM) | A12 (7D4) | PG |
TotalSeq™-C0870 anti-human CD150 (SLAM) | A12 (7D4) | PG |
Ultra-LEAF™ Purified anti-human CD150 (SLAM) | A12 (7D4) | FC |
TotalSeq™-B0870 anti-human CD150 (SLAM) Antibody | A12 (7D4) | PG |
TotalSeq™-D0870 anti-human CD150 (SLAM) | A12 (7D4) | PG |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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FITC anti-human CD150 (SLAM)
Human peripheral blood lymphocytes stained with A12 FITC -
PE anti-human CD150 (SLAM)
Human peripheral blood lymphocytes stained with A12 PE -
Purified anti-human CD150 (SLAM)
Human peripheral blood lymphocytes stained with purified A12... -
Alexa Fluor® 488 anti-human CD150 (SLAM)
Human peripheral blood lymphocytes stained with A12 Alexa Fl... -
TotalSeq™-A0870 anti-human CD150 (SLAM)
-
TotalSeq™-C0870 anti-human CD150 (SLAM)
-
Ultra-LEAF™ Purified anti-human CD150 (SLAM)
Human peripheral blood lymphocytes Ultra-LEAF™ purified A12,... -
TotalSeq™-B0870 anti-human CD150 (SLAM) Antibody
-
TotalSeq™-D0870 anti-human CD150 (SLAM)
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