- Clone
- TS1/8 (See other available formats)
- Regulatory Status
- RUO
- Workshop
- V S025
- Other Names
- LFA-2, T11, SRBC-R
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
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309235 | 100 µg | 270 CHF | ||||
309236 | 1 mg | 743 CHF |
Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.
CD2 is a 50 kD type I transmembrane glycoprotein also known as LFA-2, T11, and sheep red blood cell receptor (SRBC-R). This immunoglobulin superfamily member is expressed on thymocytes, T lymphocytes, NK cells, and thymic B cell subsets. The major ligand for CD2 is CD58 (also known as LFA-3). CD2 has also been reported to bind CD48, CD59, and CD15. CD2 plays a critical role in alternative T cell activation, T cell signaling, and cell-cell adhesion.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
- Endotoxin Level
- Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
- Preparation
- The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
- Application
-
FC - Quality tested
Block - Reported in the literature, not verified in house - Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
Additional reported applications (for the relevant formats) include: blocking of T cell activation, and partial blocking of B cell costimulation2. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 309235 & 309236).
-
Application References
(PubMed link indicates BioLegend citation) -
- Schlossman S, et al. Eds.1995. Leucocyte Typing V Oxford University Press. New York.
- Hughes CCW, et al. 1996. J. Biol. Chem. 271:5369.
- RRID
-
AB_2860824 (BioLegend Cat. No. 309235)
AB_2860824 (BioLegend Cat. No. 309236)
Antigen Details
- Structure
- Ig superfamily, type I transmembrane glycoprotein, 50 kD
- Distribution
-
T cells, NK cells, thymocytes, and thymic B cell subsets
- Function
- T cell activation, adhesion
- Ligand/Receptor
- CD58 (LFA-3), CD48, CD59, CD15
- Cell Type
- B cells, NK cells, T cells, Thymocytes
- Biology Area
- Costimulatory Molecules, Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
1. Bell G, et al. 1995. J. Immunol. 155:2805.
2. Bierer B, et al. 1989. Annu. Rev. Immunol. 7:579.
3. Moingeon P, et al. 1989. Immunol. Rev. 111:111. - Gene ID
- 914 View all products for this Gene ID
- UniProt
- View information about CD2 on UniProt.org
Related FAQs
- Do you guarantee that your antibodies are totally pathogen free?
-
BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
- Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
-
No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
- Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
-
No, BioLegend does not test for pathogens in-house unless otherwise indicated. However, we can recommend an outside vendor to perform this testing as needed.
- Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
-
We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.
Other Formats
View All CD2 Reagents Request Custom ConjugationCustomers Also Purchased
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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FITC anti-human CD2
Human peripheral blood lymphocytes stained with TS1/8 FITC -
PE anti-human CD2
Whole blood lymphocytes stained with TS1/8 PE -
Purified anti-human CD2
Human peripheral blood lymphocytes stained with purified TS1... -
Pacific Blue™ anti-human CD2
Human peripheral blood lymphocytes stained with TS1/8 Pacifi... -
PE/Cyanine7 anti-human CD2
Human peripheral blood lymphocytes stained with TS1/8 PE/Cya... -
Brilliant Violet 421™ anti-human CD2
Human peripheral blood lymphocytes stained with (clone TS1/8... -
Purified anti-human CD2 (Maxpar® Ready)
Human PBMCs stained with 151Eu anti-CD2 (TS1/8) and 170Er an... -
PerCP/Cyanine5.5 anti-human CD2
Human peripheral blood lymphocytes stained with CD3 Brillian... -
APC anti-human CD2
Human peripheral blood lymphocytes stained with CD3 Brillian... -
APC/Fire™ 750 anti-human CD2
Human peripheral blood lymphocytes stained with CD3 Brillian... -
Alexa Fluor® 700 anti-human CD2
Human peripheral blood lymphocytes stained with CD3 Brillian... -
TotalSeq™-A0367 anti-human CD2
-
TotalSeq™-C0367 anti-human CD2
-
FITC anti-human CD2
Typical results from human peripheral blood lymphocytes stai... -
TotalSeq™-B0367 anti-human CD2
-
Ultra-LEAF™ Purified anti-human CD2
Human peripheral blood lymphocytes stained with Ultra-LEAF™ ... -
APC/Cyanine7 anti-human CD2
Human peripheral blood lymphocytes were stained with anti-hu... -
PE anti-human CD2
Typical results from human peripheral blood lymphocytes stai... -
TotalSeq™-D0367 anti-human CD2
-
PE/Cyanine7 anti-human CD2
Typical results from human peripheral blood lymphocytes stai... -
PerCP/Cyanine5.5 anti-human CD2
Typical results from human peripheral blood lymphocytes stai... -
GMP PE anti-human CD2
Typical results from human peripheral blood lymphocytes stai... -
APC anti-human CD2
Typical results from human peripheral blood lymphocytes stai... -
APC/Fire™ 750 anti-human CD2
Typical results from human peripheral blood lymphocytes stai... -
Pacific Blue™ anti-human CD2
Typical results from human peripheral blood lymphocytes stai... -
GMP FITC anti-human CD2
Typical results from human peripheral blood lymphocytes stai... -
GMP APC/Fire™ 750 anti-human CD2
Typical results from human peripheral blood lymphocytes stai... -
PE/Dazzle™ 594 anti-human CD2
Human peripheral blood lymphocytes were stained with anti-hu...
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