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Ultra-LEAF™ Purified anti-human CD269 (BCMA) Antibody

Pricing & Availability
Clone
A21044E (See other available formats)
Regulatory Status
RUO
Other Names
B cell maturation antigen (BCMA), tumor necrosis factor receptor superfamily member 17, CD269
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
A21044E_ULEAF_CD269_Block_071524
Biotinylated recombinant human TNFRSF17-Fc chimera (Cat. No. 796404) (black circles) binds to immobilized recombinant human APRIL / TNFSF13 protein. Ultra-LEAF™ purified anti-human CD269 (BCMA) (clone A21044E) (purple squares) inhibits the binding in a dose-dependent manner, whereas the Ultra-LEAF™ purified mouse IgG2b, κ isotype control (Cat. No. 401216) (gray triangles) does not have the effect. This antibody blocks the binding of 0.05 µg/mL biotinylated recombinant human TNFRSF17-Fc chimera to 0.3 µg/mL immobilized recombinant human APRIL/TNFSF13. ND50 range: 0.05 - 0.3 µg/mL.
  • A21044E_ULEAF_CD269_Block_071524
    Biotinylated recombinant human TNFRSF17-Fc chimera (Cat. No. 796404) (black circles) binds to immobilized recombinant human APRIL / TNFSF13 protein. Ultra-LEAF™ purified anti-human CD269 (BCMA) (clone A21044E) (purple squares) inhibits the binding in a dose-dependent manner, whereas the Ultra-LEAF™ purified mouse IgG2b, κ isotype control (Cat. No. 401216) (gray triangles) does not have the effect. This antibody blocks the binding of 0.05 µg/mL biotinylated recombinant human TNFRSF17-Fc chimera to 0.3 µg/mL immobilized recombinant human APRIL/TNFSF13. ND50 range: 0.05 - 0.3 µg/mL.
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652154 1 mg 1045 CHF
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652153 100 µg 405 CHF
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Description

Human TNFRSF17 (B cell maturation antigen, BCMA) is a type I attached membrane protein of 184 amino acids, with a conserved motif of six cysteines in the N-terminal end, consistent with the formation of a cysteine repeat motif found in the extracellular domain of TNFRs. Overexpression of TNFRSF17 in 293 cells activates NF-kappa B, Elk-1, c-Jun N-terminal kinase, and the p38 mitogen-activated protein kinase, after its association with TNF Receptor-Associated Factor (TRAF) 1, TRAF2, and TRAF3. Soluble forms of TACI (TNFRSF13B) and TNFRSF17 bind to BAFF (TNFSFL13B) and APRIL (TNFSFL13A). TNFRSF17-APRIL interaction has higher affinity than TNFRSF17-BAFF interaction. Analysis of TNFRSF17 knockout mice (TNFRSF17−/−) demonstrates no differences in the steady-state frequencies or composition of peripheral B cell subsets (immature, marginal zone, follicular, B1). PBMCs from mice with Sjogren’s-like syndrome demonstrates upregulated TNFRSF17 expression. TNFRSF17 is also increased in patients with Sjogren’s syndrome, rheumatoid arthritis, and on B cells from SLE patients. Patients with multiple myeloma show elevated levels of TNFRSF17 in serum, and it correlated with the proportion of plasma cells in bone marrow biopsies and clinical status. Serum TNFRSF17 levels in considered a novel biomarker to predict outcomes for multiple myeloma patients.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant Human TNFRSF17-Fc Chimera
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level
Less than 0.01 EU/μg of the protein (< 0.001 ng/μg of the protein) as determined by the LAL test
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

Block - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by blocking the binding of 0.05 µg/mL biotinylated human TNFRSF17-Fc chimera (Cat. No. 796404) to immobilized 0.3 µg/mL recombinant human APRIL / TNFSF13. ND50 range:  0.05 - 0.3 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_3662332 (BioLegend Cat. No. 652154)
AB_3662332 (BioLegend Cat. No. 652153)

Antigen Details

Structure
Homodimer
Distribution

Resting and activated B cells, malignant B lymphocytes, plasma cells

Function
Maintains the peripheral B cell population and survival of long-lived bone marrow plasma cells. Soluble BCMA acts as a decoy receptor for APRIL. TNFRSF17 mRNA is stimulated by IL-4 and IL-6 in murine B cells.
Interaction
Monocytes, macrophages, T cells, and dendritic cells
Ligand/Receptor
TNFSF13 (APRIL), TNFSF (BAFF)
Cell Type
B cells, Plasma cells
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References
  1. Madry C, et al. 1998. Int Immunol. 10:1693-702.
  2. Hatzoglou A, et al. 2000. J Immunol. 165:1322-30.
  3. Rennert P, et al. 2000. J Exp Med. 192:1677-84.
  4. Wallweber HJ, et al. 2004. J Mol Biol. 343:283-90.
  5. O'Connor BP, et al. 2004. J Exp Med. 199:91-8.
  6. Kim J, et al. 2011. Autoimmunity. 44:69-81.
  7. Coqueri CM, and Erickson LD. 2012. Crit Rev Immunol. 32:287-305.
  8. Laurent SA, et al. 2015. Nature Commun. 6:7333.
  9. Ghermezi M, et al. 2017. Hematologica. 102:785-795.
Gene ID
608 View all products for this Gene ID
UniProt
View information about CD269 on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 07.15.2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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