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Ultra-LEAF™ Purified anti-human CD274 (B7-H1, PD-L1) Antibody

Pricing & Availability
Clone
W20262F (See other available formats)
Regulatory Status
RUO
Other Names
CD274, Programmed cell death 1 ligand 1 (PD-L1), B7 homolog 1 (B7h1)
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
W20262F_ULEAF_CD274_Antibody_012224
Biotinylated human PD-1 (CD279)-Fc chimera (Cat. No. 799504) (black circles) binds to immobilized recombinant human B7-H1 (PD-L1, CD274)-Fc chimera (Cat. No. 762504). Ultra-LEAF™ purified anti-human CD274 (B7-H1, PD-L1) (clone W20262F) (purple squares) inhibits the binding in a dose-dependent manner, whereas the Ultra-LEAF™ purified rat IgG2a, κ isotype control (Cat. No. 400544) (gray triangles) does not have effect. This antibody blocks the binding of 2 µg/mL biotinylated human PD-1 (CD279)-Fc chimera to 1 µg/mL immobilized recombinant human B7-H1 (PD-L1, CD274)-Fc chimera. ND50 range: 9 - 60 ng/mL.
  • W20262F_ULEAF_CD274_Antibody_012224
    Biotinylated human PD-1 (CD279)-Fc chimera (Cat. No. 799504) (black circles) binds to immobilized recombinant human B7-H1 (PD-L1, CD274)-Fc chimera (Cat. No. 762504). Ultra-LEAF™ purified anti-human CD274 (B7-H1, PD-L1) (clone W20262F) (purple squares) inhibits the binding in a dose-dependent manner, whereas the Ultra-LEAF™ purified rat IgG2a, κ isotype control (Cat. No. 400544) (gray triangles) does not have effect. This antibody blocks the binding of 2 µg/mL biotinylated human PD-1 (CD279)-Fc chimera to 1 µg/mL immobilized recombinant human B7-H1 (PD-L1, CD274)-Fc chimera. ND50 range: 9 - 60 ng/mL.
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629554 1 mg 1040 CHF
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629553 100 µg 415 CHF
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Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.

Description

PD-L1, a member of the B7 family, is a type I transmembrane protein of 290 amino acids. Human PD-L1 has 70% amino acid identity to its mouse orthologue. Binding of PD-L1 to its receptor PD-1 leads to the inhibition of T cell receptor–mediated lymphocyte proliferation and cytokine secretion. PD-L1 induces IL-10 production in T cells stimulated with low levels of anti-CD3. PD-L1/PD-1 interaction suppresses immune responses against autoantigens and tumors and plays an important role in the maintenance of peripheral immune tolerance. Disruption of the PD-L1 gene leads to up-regulated T cell responses and the generation of self-reactive T cells. Antibodies against PD-1 or PD-L1 leads to increased antitumor immunity. PD-L1 has an important role in conferring fetomaternal tolerance in an allogeneic pregnancy model. Antibodies against PD-L1 lead to a breakdown in maternal tolerance to the fetus. PD-L1 shares its receptor with PD-L2 (CD273, B7-DC). PD-L2 has a more limited expression than PD-L1, being expressed on activated macrophages and dendritic cells. PD-L1 is expressed in many tumors, and the interaction with its receptor activates signaling pathways that inhibit T-cell activity and therefore the antitumor immune response. Antibodies targeting PD-1 or PD-L1 block the PD-1 pathway and reactivate T cell activity.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant B7-H1 (PD-L1, CD274)-Fc
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level
Less than 0.01 EU/μg of the protein (< 0.001 ng/μg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

Block - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by blocking the binding of 2 µg/mL recombinant biotinylated Human PD-1 (CD279)-Fc chimera (Cat. No. 799504) to immobilized 1 µg/mL recombinant human B7-H1 (PD-L1, CD274)-Fc Chimera (Cat. No. 762504).  ND50 range: 9 - 60 ng/mL. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_3097541 (BioLegend Cat. No. 629554)
AB_3097541 (BioLegend Cat. No. 629553)

Antigen Details

Structure
Dimer
Distribution

APC, monocytes, dendritic cells, expressed in nonlymphoid tissues, and stromal cell

Function
Enhances CD28-independent T-helper cell function, suppresses immune responses against autoantigens, and participates in fetomaternal tolerance
Interaction
Antigen-stimulated T and B cells, regulatory T cells, follicular T and B cells, dendritic cells, and monocytes
Ligand/Receptor
PD-1 (CD279)
Cell Type
Astrocytes, Dendritic cells, Fibroblasts, Granulocytes, Macrophages, Monocytes, NK cells, T cells
Biology Area
Cancer Biomarkers, Costimulatory Molecules, Immunology
Molecular Family
Adhesion Molecules, CD Molecules, Immune Checkpoint Receptors
Antigen References
  1. Dong H, et al. 1999. Nat Med. 5:1365-9.
  2. Freeman GJ, et al. 2000. J Exp Med. 192:1027-34.
  3. Latchman Y, et al. 2001. Nat. Immunol. 2:261-8.
  4. Latchman YE, et al. 2004. Proc. Natl Acad Sci. USA. 101:10691.
  5. Guleria I, et al. 2005. J Exp Med. 202:231-7.
  6. Lin DY, et al. 2008. Proc Natl Acad Sci. USA. 105:3011.
  7. Dai S, et al. 2014. Cell Immunol. 290:72-9.
  8. Melero I, et al. 2015. Nat Rev Cancer. 15:457-72.
Gene ID
29126 View all products for this Gene ID
UniProt
View information about CD274 on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 01.22.2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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