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Ultra-LEAF™ Purified anti-human CD41/CD61 Antibody

Pricing & Availability
Clone
A2A9/6 (See other available formats)
Regulatory Status
RUO
Other Names
gpIIb/gpIIIa
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
A2A9_Purified_CD41CD61_Antibody_FC_1_082913.jpg
Thrombin-activated human peripheral blood platelets were stained with mouse IgG1 anti-CD62P APC and purified CD41/CD61 (clone A2A9/6) (top) or purified mouse IgG2a, κ isotype control (bottom), followed by anti-mouse IgG2a PE.
  • A2A9_Purified_CD41CD61_Antibody_FC_1_082913.jpg
    Thrombin-activated human peripheral blood platelets were stained with mouse IgG1 anti-CD62P APC and purified CD41/CD61 (clone A2A9/6) (top) or purified mouse IgG2a, κ isotype control (bottom), followed by anti-mouse IgG2a PE.
  • A2A9_Purified_CD41CD61_Antibody_FC_2_082913.jpg
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359804 500 µg 325 CHF
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Description

CD41/CD61, also known as gpIIb/IIIa, is a member of a family integrin receptors. This is a complex comprised by CD41 and CD61 through non-covalent association. CD41/CD61 is mainly expressed by platelets and megakaryocytes. The resting form of the CD41/CD61 complex is involved in platelet activation and aggregation by binding to immobilized fibrinogen. After activation, CD41/CD61 becomes a receptor for soluble fibrinogen and several other RGD-containing adhesive proteins such as von Willebrand Factor (vWF) and fibronectin. An absence or dysfunction of CD41/CD61 on the platelet surface results in an inherited bleeding disorder, called Glanzmann Thromsasthenia (GT). CD41/CD61 has been found on murine hematopoietic progenitor cells, indicating that this complex may play a role in regulating hematopoietic development.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human platelets
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level
Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

FC - Quality tested
Block, IP - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for relevant formats) include: immunoprecipitation1 and blocking (aggregation)2. The Ultra-LEAF™ purified antibody (Endotoxin <0.01 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 359804).

A2A9/6 is recognized as having high affinity to gpIIb/IIIa. It has been shown to inhibit platelet aggregation induced by a variety of agonists and the initiation of clot formation.

Application References

(PubMed link indicates BioLegend citation)
  1. Basani RB, et al. 1996. Blood. 88:167. (IP)
  2. Bennett JS, et al. 1983. Proc. Natl. Acad. Sci. USA. 80:2417. (Block)
Product Citations
  1. Zheng L, et al. 2021. Front Cell Infect Microbiol. 11:783085. PubMed
  2. Posner M, et al. 2016. J Biol Chem. 291: 2764 - 2776.. PubMed
RRID
AB_2562484 (BioLegend Cat. No. 359804)

Antigen Details

Structure
Complex of CD41 and CD61 through non-covalent association.
Distribution

Platelets and megakaryocytes.

Function
Mediates attachment to cells of diverse matrix proteins.
Ligand/Receptor
Fibrinogen, RGD-containing adhesive proteins such as vonWillebrand Factor (vWF) and fibronectin.
Cell Type
Megakaryocytes, Platelets
Biology Area
Cell Adhesion, Cell Biology, Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Matsumura-Takeda K, et al. 2007. Stem Cell. 25:862.
2. Corbel C, et al. 2005. 49:279.
3. Bennett JS, et al. 1983. Proc. Natl. Acad. Sci. USA. 80:2417. 
4. Clemetson KJ, et al. 1994. Curr. Opin. Hematol. 1:388.

Gene ID
3690 View all products for this Gene ID 3674 View all products for this Gene ID
UniProt
View information about CD41/CD61 on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 09.06.2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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