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Ultra-LEAF™ Purified anti-human CXCL13 (BLC) Antibody

Pricing & Availability
Clone
A15151D (See other available formats)
Regulatory Status
RUO
Other Names
B cell attracting chemokine (BCA-1), B lymphocyte chemoattractant
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
A15151D_ULEAF_CXCL13_Antibody_1_021920
Recombinant human CXCL13 (black circles) (Cat. No. 574706) induces the chemotaxis of mouse Baf3-hCXCR5 transfectants chemoattractant cells. Ultra-LEAF™ purified anti-human CXCL13 (BLC) antibody (purple squares) (clone A15151D) neutralizes the chemotactic activity of recombinant human CXCL13 on mouse Baf3-hCXCR5 cells in a dose-dependent manner while the isotype control (grey triangles) (Cat. No. 400165) does not have the effect. ND50 range: 0.1 - 1.5 µg/mL.
  • A15151D_ULEAF_CXCL13_Antibody_1_021920
    Recombinant human CXCL13 (black circles) (Cat. No. 574706) induces the chemotaxis of mouse Baf3-hCXCR5 transfectants chemoattractant cells. Ultra-LEAF™ purified anti-human CXCL13 (BLC) antibody (purple squares) (clone A15151D) neutralizes the chemotactic activity of recombinant human CXCL13 on mouse Baf3-hCXCR5 cells in a dose-dependent manner while the isotype control (grey triangles) (Cat. No. 400165) does not have the effect. ND50 range: 0.1 - 1.5 µg/mL.
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934503 100 µg 270 CHF
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934504 1 mg 715 CHF
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Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.

Description

CXCL13 was initially described as a chemokine highly expressed in the follicles of Peyer's patches, spleen, and lymph nodes and as a strong chomoattractant for B cells. CXCL13 is expressed by T follicular helper cells, dendritic cells, and stromal cells in B cell areas of secondary lymphoid tissue. CXCR5, the receptor for CXCL13, was initially cloned from Burkitt’s lymphoma cells and named BLR1. CXCR5 is highly expressed in those cells and B cells. CXCL13, CCL19, and CCL21 are involved in the formation of tertiary lymphoid tissues in chronic antigen-induced arthritis. CXCL13 takes part in the allergic airway inflammatory process. In addition, high expression of CXCL13 has been linked to lupus, Helicobacter pylori-associated gastritis, multiple sclerosis, and rheumatoid arthritis. In summary, CXCL13 is highly expressed at the sites of new lymphoid tissue formation in a variety of chronic inflammatory conditions.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant Human CXCL13
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level
Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

Neut - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by neutralization of the chemotaxis induced by recombinant human CXCL13 on mouse Baf3-hCXCR5 transfectants chemoattractant cells. ND50 range: 0.1 - 1.5 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

Product Citations
  1. Webb ER, et al. 2023. Elife. :12. PubMed
RRID
AB_2832886 (BioLegend Cat. No. 934503)
AB_2832886 (BioLegend Cat. No. 934504)

Antigen Details

Structure
Chemokine
Distribution

Follicular dendritic cells, monocytes, macrophages, and stromal cells from germinal centers. CXCL13 is derived from endothelial cells, synovial fibroblasts, and follicular dendritic cells in RA.

Function
CXCL13 chemoattracts B cells and is a key chemokine for B cell entry into lymphoid follicles. It also takes part in the formation of ectopic lymphoid tissue in chronic inflammation. CXCL13 production by follicular Th cells is induced by TCR activation.
Interaction
B cells and follicular helper CD4 T cells
Ligand/Receptor
CXCR5 (CD185)
Cell Type
B cells, Macrophages, T cells
Biology Area
Cell Biology, Immuno-Oncology, Immunology, Signal Transduction
Molecular Family
Cytokines/Chemokines
Antigen References
  1. Gunn MD, et al. 1998. Nature. 391:799.
  2. Legler DF, et al. 1998. J Exp Med. 187:655.
  3. Kim CH, et al. 2004. Blood. 104:1952.
  4. McDonald KG, et al. 2010. Am J Pathol. 176:2367.
  5. Gatto D, et al. 2011. J Immunol. 187:4621.
Gene ID
10563 View all products for this Gene ID
UniProt
View information about CXCL13 on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 2    Revision Date: 09.26.2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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