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Ultra-LEAF™ Purified anti-human Noggin Antibody

Pricing & Availability
Clone
A15144A (See other available formats)
Regulatory Status
RUO
Other Names
SYM1, SYNS1, NOG, Symphalangism 1, SYNS1A
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
A15144A_ULEAF_Noggin_Antibody_09182
Recombinant Human Noggin (Cat. No. 795904) (black circles) inhibits Recombinant Human BMP-4 (Cat. No. 795604) induced alkaline phosphatase production of ATDC5 mouse chondrogenic cells. Ultra-LEAF™ purified anti-human Noggin (clone A15144A) (purple squares) neutralizes the effect of recombinant human Noggin at 0.5 µg/mL and human BMP-4 at 50 ng/mL in a dose-dependent manner, whereas the Ultra-LEAF™ purified mouse IgG2b, κ isotype control (Cat. No. 401216) (gray triangles) does not have this effect. ND50 range: 0.3-2 µg/mL
  • A15144A_ULEAF_Noggin_Antibody_09182
    Recombinant Human Noggin (Cat. No. 795904) (black circles) inhibits Recombinant Human BMP-4 (Cat. No. 795604) induced alkaline phosphatase production of ATDC5 mouse chondrogenic cells. Ultra-LEAF™ purified anti-human Noggin (clone A15144A) (purple squares) neutralizes the effect of recombinant human Noggin at 0.5 µg/mL and human BMP-4 at 50 ng/mL in a dose-dependent manner, whereas the Ultra-LEAF™ purified mouse IgG2b, κ isotype control (Cat. No. 401216) (gray triangles) does not have this effect. ND50 range: 0.3-2 µg/mL
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620454 1 mg 1225 CHF
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620453 100 µg 380 CHF
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Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.

Description

Noggin (NOG) is an embryonic inducer that can sequester TGF-β cytokines of the BMP family with extremely high affinity. This protein was originally identified as a BMP-4 antagonist whose action is critical for proper formation of the head and other dorsal structures. NOG also has been shown to modulate the activities of other BMPs including BMP-2, -7, -13, and -14. It is expressed in defined areas of the adult central nervous system and peripheral tissues such as lung, skeletal muscle, and skin. During culture of human embryonic stem cells (human ESC) or neural stem cells under certain conditions, addition of NOG to antagonize BMP activity may allow stem cells to proliferate while maintaining their undifferentiated state, or alternatively, to differentiate into dopaminergic neurons. NOG also appears to maintain adult stem cell populations in vivo. For example, it maintains neural stem cells within the hippocampus. Targeted deletion of NOG in mice results in prenatal death and recessive phenotype displaying a severely malformed skeletal system. Conversely, transgenic mice over-expressing NOG in mature osteoblasts display impaired osteoblastic differentiation, reduced bone formation, and severe osteoporosis.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant human Noggin
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level
Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

Neut - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by neutralizing alkaline phosphatase production of ATDC5 mouse chondrogenic cells which was inhibited by recombinant human Noggin (Cat. No. 795904) at 0.5 µg/mL and human BMP-4 (Cat. No. 795604) at 50 ng/ml. ND50 range: 0.3-2 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_3083405 (BioLegend Cat. No. 620454)
AB_3083405 (BioLegend Cat. No. 620453)

Antigen Details

Structure
Cytokine
Distribution

Skeletal muscles, lungs, neurons, and skin

Function
Antagonizes BMPs
Interaction
Mesenchymal stem cells
Ligand/Receptor
BMPs
Cell Type
Embryonic Stem Cells, Mesenchymal Stem Cells
Biology Area
Cell Biology, Immunology, Neuroscience, Signal Transduction, Stem Cells, Synaptic Biology
Molecular Family
Cytokines/Chemokines, Growth Factors
Antigen References
  1. Valenzuela DM, et al. 1995. J Neurosci. 15:6077-84.
  2. McMahon JA, et al. 1998. Genes Dev. 12:1438-52.
  3. Yuasa S, et al. 2005. Nat Biotechnol. 23:607-11.
  4. Gong Y, et al. 1999. Nat Genet. 21:302-4.
  5. Chiba S, et al. 2008. Stem Cells. 26:2810-20.
  6. Bonaguidi MA, et al. 2008. J Neurosci. 28:9194-204.
Gene ID
9241 View all products for this Gene ID
UniProt
View information about Noggin on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 07.18.2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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