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Ultra-LEAF™ purified anti-human PLGF Antibody

Pricing & Availability
Clone
W22016A (See other available formats)
Regulatory Status
RUO
Other Names
Placental growth factor 1 (PGF1)
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
W22016A_ULEAF_PLGF_Block_081224
Recombinant human VEGFR1 (Cat. No. 555804) (black circles) binds to immobilized recombinant human PLGF (Cat. No. 590704). Ultra-LEAF™ purified anti-human PLGF (clone W22016A) (purple squares) inhibits the binding in a dose-dependent manner, whereas the Ultra-LEAF™ purified rat IgG2a, κ isotype control (Cat. No. 400544) (gray triangles) does not have the effect. This antibody blocks the binding of 0.25 µg/mL human VEGFR1 to 0.5 µg/mL immobilized recombinant human PLGF (Cat. No. 590704). ND50 range: 0.06 - 0.35 µg/mL.
  • W22016A_ULEAF_PLGF_Block_081224
    Recombinant human VEGFR1 (Cat. No. 555804) (black circles) binds to immobilized recombinant human PLGF (Cat. No. 590704). Ultra-LEAF™ purified anti-human PLGF (clone W22016A) (purple squares) inhibits the binding in a dose-dependent manner, whereas the Ultra-LEAF™ purified rat IgG2a, κ isotype control (Cat. No. 400544) (gray triangles) does not have the effect. This antibody blocks the binding of 0.25 µg/mL human VEGFR1 to 0.5 µg/mL immobilized recombinant human PLGF (Cat. No. 590704). ND50 range: 0.06 - 0.35 µg/mL.
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647654 1 mg 1045 CHF
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647653 100 µg 405 CHF
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Description

Placenta growth factor (PLGF) was initially cloned form a term placenta cDNA library. PLGF is a dimeric glycoprotein with 53% homology to VEGF. As a result of alternative splicing, four isoforms for PLGF has been described, PLGF-1, PLGF-2, PLGF-3, and PLGF-4.  PLGF-2 binds to heparin with high affinity, whereas neither PLGF-1, PLGF-3, nor PLGF-4 bind heparin. PLGF-2 is the only isoform in mouse. PLGF binds to VEGFRI but not to VEGFRII; also, PLGF binds to sVEGF1. PLGF plays a key role in the maternal vascular function during pregnancy. Low levels of PLGF have been associated with patients experiencing preeclampsia and delivering small-for-gestational age (SGA) neonates. High expression of PLGF has been associated to colorectal cancer (CRC), and its expression in CRC tissues correlates with high levels of MMP-9 and VEGF1. PLGF has been shown to increase tumor cell migration in lung cancer, leukemia, and melanoma cells. In colorectal cancer, PLGF induces tumor cell migration and invasion through upregulation of MMP9. PLGF is expressed in the majority of medulloblastomas, and high expression of PLGF receptor neuropilin 1 (Nrp1) correlates with poor survival in patients. In a mouse model, PLGF/Nrp1 blockade provided direct antitumor effects in vivo, medulloblastoma regression, decreased metastasis, and increased survival.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant human PLGF
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level
Less than 0.01 EU/μg of the protein (less than 0.001 ng/μg of the protein) as determined by the LAL test
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

Block - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by blocking the binding of 0.25 µg/mL recombinant human VEGFR1 (Cat. No. 555804) to immobilized 0.5 µg/mL recombinant human PLGF (Cat. No. 590704). ND50 range:  0.06 - 0.35 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_3662330 (BioLegend Cat. No. 647654)
AB_3662330 (BioLegend Cat. No. 647653)

Antigen Details

Structure
Homodimer
Distribution

Vascular endothelial cells, smooth muscle cells, keratinocytes, hematopoitic cells, retinal pigment epithelial cells, tumor cells, and cerebellar stroma cells

Function
PLGF is an angiogenic factor and induces proliferation and migration of endothelial cells. It stimulates endothelial cells and synergistically amplifies the action of VEGF. Soluble VEGFR1 (sFLT1) can regulate the accessibility of PLGF.
Interaction
Endothelial cells, Macrophages, and trophoblast
Ligand/Receptor
VEGFR1 (FLT-1), and neuropilin 1 (Nrp1)
Cell Type
Hematopoietic stem and progenitors
Biology Area
Cell Biology, Signal Transduction, Stem Cells
Molecular Family
Cytokines/Chemokines, Growth Factors
Antigen References
  1. Maglione D, et al. 1991. Proc Natl Acad Sci USA. 88:9267-71.
  2. Carmeliet P, et al. 2001. Nat Med. 7:575-83.
  3. Casalou C, et al. 2007. Leukemia. 21:1590-4.
  4. Marcellini M, et al. 2006. Am J Pathol. 169:643-54.
  5. Yao J, et al. 2011. Proc Natl Acad Sci USA. 108:11590.
  6. Cai J, et al. 2011. PLoS One. 6:e18076.
  7. Sibiude J, et al. 2012. PLoS One. 7:e50208.
  8. Snuderl M, et al. 2013. Cell. 152:1065-76.
Gene ID
5228 View all products for this Gene ID
UniProt
View information about PLGF on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 07.23.2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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