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- Clone
- W21039F (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Fms-related tyrosine kinase 1, oncogene FLT, FLT, FLT-1, VEGFR1, VEGFR-1, VEGFR
- Isotype
- Rat IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
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Biotinylated recombinant human VEGF165 (black circles) binds to immobilized recombinant human VEGFR1 (Cat. No. 555804). Ultra-LEAF™ purified anti-human VEGFR1 (clone W21039F) (purple squares) inhibits the binding in a dose-dependent manner, whereas the Ultra-LEAF™ purified rat IgG2b, κ isotype control (Cat. No. 400644) (gray triangles) does not have this effect. This antibody blocks the binding of 6 ng/mL Biotinylated human VEGF165 to 1 µg/mL immobilized recombinant human VEGFR1. ND50 range: 1 - 6 µg/mL.
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 652754 | 1 mg | 1175 CHF | ||||
| 652753 | 100 µg | 470 CHF | ||||
The VEGF family includes five members, VEGFA (VEGFA165), VEGFB, VEGFC, VEGFD and placenta growth factor (PLGF). Structurally, these growth factors are homodimers although heterodimers have been described that are composed of VEGFA and PLGF. There are three VEGF receptor tyrosine kinases: VEGFR1 (Flt1), VEGFR2 (Flk1), and VEGFR3 (Flt4). The expression of these receptors is overlapping; nevertheless, a distribution pattern has been reported: VEGFR1 is expressed on monocytes and macrophages, VEGFR2 on vascular endothelial cells, and VEGFR3 on lymphatic endothelial cells. VEGFR1 and VEGFR2 share 43.2% amino acid sequence homology and are structurally similar. VEGFR1 is an angiogenic and anti-angiogenic factor depending on the ligand binding it (PLGF-1 inhibits angiogenesis, whereas VEGF and PLGF2 are pro-angiogenic). Also, VEGFR1 mediates the angiostatic effect of pigment epithelium-derived factor (PEDF). A soluble form of VEGFR1 was initially identified in conditioned culture medium of HUVEC cells and it was found that it binds with high affinity to VEGFA. Soluble VEGFR1 and VEGFR2 act as decoy receptors for VEGFA, regulating the availability of VEGFA of which there are at least four different alternatively spliced soluble proteins. sVEGFR1 expression is upregulated by hypoxic conditions via HIF1-α in cytotrophoblasts. In preeclampsia activated platelets bind to monocytes to produce sVEGFR1. Increased circulating sVEGFR1 has been correlated with reduced free PLGF and reduced free VEGF in the blood compared to normal pregnancies. In addition, sVEGFR1 has been detected in cornea, and it is essential for preserving the avascular ambit of the cornea.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Recombinant Human VEGFR1
- Formulation
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
- Endotoxin Level
- Less than 0.01 EU/μg of the protein (less than 0.001 ng/μg of the protein) as determined by the LAL test
- Preparation
- The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
- Application
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Block - Quality tested
- Recommended Usage
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Each lot of this antibody is quality control tested by blocking the binding of 6 ng/mL recombinant biotinylated human VEGF165 to immobilized 1 µg/mL recombinant human VEGFR1 (Cat. No. 555804). ND50 range: 1 - 6 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
Antigen Details
- Structure
- Cytokine receptor
- Distribution
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Endothelial cells, placental trophoblast cells, peripheral blood monocytes and macrophages
- Function
- VEGFR1 plays a key role in angiognesis and vasculogenesis. It is regulated by PEDF, which effects its intracellular proteolysis rate. sVEGFR1 regulates the availability of VEGFA and is produced in the placenta under hypoxic conditions.
- Interaction
- Hematopoietic stem and progenitors, Mesenchymal Stem Cells, Neural Stem Cells
- Ligand/Receptor
- VEGFR-A, VEGFR-B, and PlGF
- Cell Type
- Hematopoietic stem and progenitors, Mesenchymal Stem Cells, Neural Stem Cells
- Biology Area
- Angiogenesis, Cell Biology, Neuroscience, Stem Cells, Synaptic Biology
- Molecular Family
- Cytokine/Chemokine Receptors, Soluble Receptors
- Antigen References
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- Kendall RL, et al. 1996. Biochem Biophys Res Commun. 226:324-8..
- Cai J, et al. 2006. J Biol Chem. 281:3604.
- Ambati BK, et al. 2006. Nature. 443:993-7.
- Heydarian M, et al. 2009. Placenta. 30:250-5.
- Thomas CP, et al. 2009. J Clin Endocrinol Metab. 94:2524-30.
- Wu, et al. 2010. J Cell Mol Med. 14:528-52.
- Cai J, et al. 2011. PLoS One. 6:e18076.
- Gene ID
- 2321 View all products for this Gene ID
- UniProt
- View information about VEGFR1 on UniProt.org
Related Pages & Pathways
Pathways
Related FAQs
- Do you guarantee that your antibodies are totally pathogen free?
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BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
- Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
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No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
- Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
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No, BioLegend does not test for pathogens in-house unless otherwise indicated. However, we can recommend an outside vendor to perform this testing as needed.
- Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
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We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.
Other Formats
View All VEGFR1 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Ultra-LEAF™ Purified anti-human VEGFR1 | W21039F | Block |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Ultra-LEAF™ Purified anti-human VEGFR1
Biotinylated recombinant human VEGF165 (black circles) binds...
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