Ultra-LEAF™ Purified anti-mouse CD178.1 (FasL) Antibody

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

C57BL/6 mouse FasL cDNA-transfected L5178Y cells

Formulation

0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.

Endotoxin Level

Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.

Preparation

The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.

Concentration

The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: in vitro blocking^1,2 and in vivo blocking^3-5, and immunohistochemical staining⁶ of formalin-fixed paraffin-embedded tissue sections. Fas Ligand is expressed at low density on activated cells. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 106806) or by using a high sensitivity, three-layer staining technique (e.g., including biotinylated anti-mouse IgG (Cat. No. 405303) second step , followed by SAv-PE (Cat. No. 405204)). The Ultra-LEAF™ purified antibody (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 106809 and 106810).

Application References

(PubMed link indicates BioLegend citation)

1. Kayagaki N, et al. 1997. P. Natl. Acad. Sci. USA 94:3914.
2. Kobata T, et al. 1997. Immunology 92:206.
3. Hattori K, et al. 1998. Blood 91:4051.
4. Takeda Y, et al. 1998. Diabetologia 41:315.
5. Kim YH, et al. 1999. Eur. J. Immunol. 29:455.
6. Takeda Y, et al. 1998. Diabetologia 41:315.

RRID

AB_2819812 (BioLegend Cat. No. 106809)
AB_2819812 (BioLegend Cat. No. 106810)

Antigen Details

Structure

TNF superfamily Fas ligand alloantigen, 40 kD

Distribution

Activated T cells, spleen, testis, eye of some selected mouse strains (C57BL/6, C3H, NZB, NZw, MRL)

Function

Induces apoptosis

Ligand/Receptor

Fas (CD95)

Cell Type

T cells, Tregs

Biology Area

Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Neuroscience

Molecular Family

CD Molecules

Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Nagata S. 1999. Annu. Rev. Genet. 33:209.
3. Takahashi T, et al. 1994. Cell 76:969.
4. Hill LL, et al. 1999. Science 285:898.
5. Kayagaki N, et al. 1997. P. Natl. Acad. Sci. USA 94:3914.

Gene ID

14103 View all products for this Gene ID

UniProt

View information about CD178.1 on UniProt.org

Documentation

• Technical Data Sheet (pdf)
• Certificate of Analysis
• Safety Data Sheet

Reviews

Related Protocols

• Cell Surface Flow Cytometry Staining Protocol

Related Pages & Pathways

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Other Formats

Certificate of Analysis