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Ultra-LEAF™ Purified anti-mouse CD198 (CCR8) Antibody

Pricing & Availability
Clone
SA214G2 (See other available formats)
Regulatory Status
RUO
Other Names
CDw198, C-C chemokine receptor type 8, CKR-8, CC-CKR-8, Ter1
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
SA214G2_ULEAF_CD198_CCR8_Antibody_053123
C57BL/6 mouse thymocytes were stained with Ultra-LEAF™ purified anti-mouse CD198 (CCR8) (clone SA214G2) (right), or Ultra-LEAF™ purified rat IgG2b, κ isotype control (left), followed by anti-rat IgG-PE and then by anti-mouse CD4 APC.
  • SA214G2_ULEAF_CD198_CCR8_Antibody_053123
    C57BL/6 mouse thymocytes were stained with Ultra-LEAF™ purified anti-mouse CD198 (CCR8) (clone SA214G2) (right), or Ultra-LEAF™ purified rat IgG2b, κ isotype control (left), followed by anti-rat IgG-PE and then by anti-mouse CD4 APC.
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150341 100 µg 270 CHF
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150342 1 mg 743 CHF
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Description

C-C chemokine receptor type 8 (CCR8) CD198, is a 41 kD G-protein coupled receptor with 7 transmembrane regions. CCR8 is expressed by a subset of thymocytes, Tregs, NKT and Th2-polarized cells, a subset of macrophages, monocytes, and monocyte-derived dendritic cells. CCR8 mediates chemotaxis toward its ligand CCL1, and is involved in apoptosis of thymocytes.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse CD198 (CCR8)-transfected cells
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level
Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_3105975 (BioLegend Cat. No. 150341)
AB_3105975 (BioLegend Cat. No. 150342)

Antigen Details

Structure
G-protein coupled receptor, 7 transmembrane regions, 41 kD.
Distribution

Thymocytes, Tregs, NKT and Th2-polarized cells, subset of macrophages, monocytes and monocyte-derived dendritic cells.

Function
Chemotaxis, involved in thymocyte apoptosis
Ligand/Receptor
CCL1 (SCY1,TCA-3)
Cell Type
Dendritic cells, Macrophages, Monocytes, NKT cells, Th2, Thymocytes, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors, GPCR
Antigen References

1. Coghill JM, et al. 2013. Blood. 122:825.
2. Islam SA, et al. 2011. Nat. Immunol. 12:167.
3. Hoshino A, et al. 2007. J. Immunol. 178:5296.
3. Qu C, et al. 2004. J. Exp. Med. 200:1231.

Gene ID
12776 View all products for this Gene ID
UniProt
View information about CD198 on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 05.31.2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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