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Ultra-LEAF™ Purified anti-mouse CD200R (OX2R) Antibody

Pricing & Availability
Clone
OX-110 (See other available formats)
Regulatory Status
RUO
Other Names
OX-2R, Orexin Receptor 2 (OX2R), CD200R
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
OX-110_LEAF_CD200R_Antibody_FC_032613
C57BL/6 bone marrow cells were stained with Ultra-LEAF™ purified anti-mouse CD200R (OX2R) (clone OX-110) or rat IgG2a, κ isotype control, followed by anti-rat IgG PE. Data shown was gated on myeloid cell population.
  • OX-110_LEAF_CD200R_Antibody_FC_032613
    C57BL/6 bone marrow cells were stained with Ultra-LEAF™ purified anti-mouse CD200R (OX2R) (clone OX-110) or rat IgG2a, κ isotype control, followed by anti-rat IgG PE. Data shown was gated on myeloid cell population.
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123917 100 µg 237 CHF
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123918 1 mg 743 CHF
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Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.

Description

CD200R, also known as OX2R, is a membrane glycoprotein with up to 70% of its weight derived from N-linked glycosylation. CD200R is expressed primarily by monocytes and neutrophils but also by other leukocytes including T lymphocytes and mast cells. The interaction between CD200 and CD200R may contribute to pathways that suppress and limit macrophage induced inflammatory damage in tissue. Studies suggest that CD200-CD200R interaction may be involved in the control of myeloid cellular function. Levels of expression on resting peripheral blood cells are relatively low.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Fusion protein mouse CD200RCD4d3
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level
Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

FC - Quality tested
FA - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional applications (for the relevant formats) include: in vivo studies3. The Ultra-LEAF™ purified antibody is recommended for functional assays.

Application References

(PubMed link indicates BioLegend citation)
  1. Voulgaraki D, et al. 2005. Immunol. 115:337
  2. Stumper TL, et al. 2011. J. Immunol. 186:1970. PubMed
  3. Talebian F, et al. 2012. PLoS One 7:e31442. (FA)
RRID
AB_2890680 (BioLegend Cat. No. 123917)
AB_2890680 (BioLegend Cat. No. 123918)

Antigen Details

Distribution

Monocytes and neutrophils, T lymphocytes and mast cells

Cell Type
Mast cells, Monocytes, Neutrophils, T cells
Biology Area
Immunology, Inhibitory Molecules
Molecular Family
CD Molecules
Antigen References

1. Voulgaraki D, et al. 2005. Immunol. 115:337

Gene ID
57781 View all products for this Gene ID
UniProt
View information about CD200R on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 11.13.2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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