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Ultra-LEAF™ Purified anti-mouse CD262 (DR5, TRAIL-R2) Antibody

Pricing & Availability
Clone
MD5-1 (See other available formats)
Regulatory Status
RUO
Other Names
DR5, TRAIL-R2, TNFRS10B, TRICK2, KILLER
Isotype
Armenian Hamster IgG
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Product Citations
publications
MD5-1_LEAF_090707
Mouse DR5 transfected cells stained with Ultra-LEAF™ purified MD5-1, followed by biotinylated anti-Armenian hamster IgG and Sav-PE
  • MD5-1_LEAF_090707
    Mouse DR5 transfected cells stained with Ultra-LEAF™ purified MD5-1, followed by biotinylated anti-Armenian hamster IgG and Sav-PE
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119909 100 µg 110 CHF
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Description

CD262 is a 55 kD protein also known as DR5, TRAIL-R2, Apo-2, TRICK2, and KILLER. DR5 is member 10B of the TNF receptor superfamily (TNFRSF10B), widely expressed in normal tissues and some tumor cell lines. The DR5 receptor binds to TRAIL and interacts with the cytoplasmic proteins TRADD and RIP. TRAIL binding to DR5 activates NF- κB and induces apoptosis. DR5 is induced by wild-type p53.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
Mouse DR5-Ig fusion protein
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level
Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

FC - Quality tested
Cyt - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: in vitro and in vivo induction of cytotoxicity2,3. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 119909).

Application References

(PubMed link indicates BioLegend citation)
  1. Hayakawa Y, et al. 2004. J. Immunol. 172:123. (FC)
  2. Takeda K, et al. 2004. J. Exp. Med. 199:437. (Cyt)
  3. Sato K, et al. 2005. J. Immunol. 174:4025. (Cyt)
  4. Iannello A, et al. 2009. J. Virol. 83:5999. PubMed
  5. Grisendi G, et al. 2010. Cancer Res. 70:3718. PubMed
RRID
AB_2890679 (BioLegend Cat. No. 119909)

Antigen Details

Structure
Type I transmembrane protein, TNF receptor superfamily member, homotrimer, predicted molecular weight 55 kD
Distribution

Widely expressed in normal tissues and some tumor cell lines. Expression regulated by the tumor suppressor p53

Function
Binds to TRAIL, activates NF- ?B, induces apoptosis. May be involved in the regulation of tumor metastases.
Ligand/Receptor
TRAIL (CD253); cytoplasmic domain interacts with TRADD and RIP
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors
Antigen References

1. Wu GS, et al. 1999. Cancer Res. 59:2770.

Gene ID
21933 View all products for this Gene ID
UniProt
View information about CD262 on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 02.19.2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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