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Ultra-LEAF™ Purified anti-mouse CD44 Antibody

Pricing & Availability
Clone
W19210A (See other available formats)
Regulatory Status
RUO
Other Names
Hyaluronate receptor
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
W19210A_ULEAF_CD44_Antibody_1_122022
Murine B-cells were isolated using the MojoSort™ Mouse Pan B Cell Isolation Kit (Cat. No. 480051) and activated overnight with 20 ng/mL recombinant mouse IL-4 (carrier-free) (Cat. No. 574302) and 10 µg/mL purified anti-mouse IgM (Cat. No. 406501). Activated B cells were seeded onto tissue culture plates pre-coated with 20 µg/mL Ultra-LEAF™ purified anti-mouse CD44 (clone W19210A) or isotype control. After 4 hours, the number of elongated cells (circularity < 0.5) was quantified using brightfield imaging and morphometric analysis with ImageJ. The assay was performed in triplicate, and 4 images per well at 20x magnification were analyzed. Data shown are normalized to rat IgG2a, κ isotype control treated wells.
  • W19210A_ULEAF_CD44_Antibody_1_122022
    Murine B-cells were isolated using the MojoSort™ Mouse Pan B Cell Isolation Kit (Cat. No. 480051) and activated overnight with 20 ng/mL recombinant mouse IL-4 (carrier-free) (Cat. No. 574302) and 10 µg/mL purified anti-mouse IgM (Cat. No. 406501). Activated B cells were seeded onto tissue culture plates pre-coated with 20 µg/mL Ultra-LEAF™ purified anti-mouse CD44 (clone W19210A) or isotype control. After 4 hours, the number of elongated cells (circularity < 0.5) was quantified using brightfield imaging and morphometric analysis with ImageJ. The assay was performed in triplicate, and 4 images per well at 20x magnification were analyzed. Data shown are normalized to rat IgG2a, κ isotype control treated wells.
  • W19210A_ULEAF_CD44_Antibody_2_010923
    Murine B-cells were isolated using the MojoSort™ Mouse Pan B Cell Isolation Kit (Cat. No. 480051) and activated overnight with 20 ng/mL recombinant mouse IL-4 (carrier-free) (Cat. No. 574302) and 10 µg/mL purified anti-mouse IgM (Cat. No. 406501). Activated B cells were seeded onto IBIDI® µ-Slide Glass Bottom slides pre-coated with 20 µg/mL Ultra-LEAF™ purified anti-mouse CD44 (clone W19210A) (left) or isotype control (right). After 4 hours, cells were fixed with Fixation Buffer (Cat. No. 420801) and stained with Flash Phalloidin™ Green 488 (Cat. No. 424201) in Intracellular Staining Permeabilization Wash Buffer (10X) (Cat. No. 421002). Images were taken using a 40x objective.
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112304 1 mg 1205 CHF
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112303 100 µg 325 CHF
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Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.

Description

CD44 is an 80-95 kD glycoprotein also known as Hermes, Pgp1, H-CAM, or HUTCH. It is expressed on all leukocytes, endothelial cells, hepatocytes, and mesenchymal cells. As B and T cells become activated or progress to the memory stage, CD44 expression increases from low or mid levels to high levels. Thus, CD44 has been reported to be a valuable marker for memory cell subsets. High CD44 expression on Treg cells has been associated with potent suppressive function via high production of IL-10. CD44 is an adhesion molecule involved in leukocyte attachment to and rolling on endothelial cells, homing to peripheral lymphoid organs and to the sites of inflammation, and leukocyte aggregation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant mouse CD44 protein, aa 25-224
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level
Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

Activ - Quality tested
Recommended Usage

Each lot of this clone is quality control tested by induction of spreading of activated murine B-cells on a tissue culture plate coated with 20 µg/mL anti-mouse CD44. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This clone activates CD44 on murine B-cells as shown by induction of B-cell spreading on anti-mouse CD44 coated surface.

RRID
AB_2936697 (BioLegend Cat. No. 112304)
AB_2936697 (BioLegend Cat. No. 112303)

Antigen Details

Structure
Variable splicing of CD44 gene generates many CD44 isoforms, 80-95 kD
Distribution

All leukocytes, epithelial cells, endothelial cells, hepatocytes, mesenchymal cells

Function
Leukocyte attachment and rolling on endothelial cells, stromal cells and ECM
Ligand/Receptor
Hyaluronan, MIP-1β, fibronectin, collagen
Cell Type
B cells, Endothelial cells, Epithelial cells, Leukocytes, Mesenchymal cells
Biology Area
Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References
  1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
  2. Haynes BF, et al. 1991. Cancer Cells. 3:347-50.
  3. Goldstein LA, et al. 1989. Cell. 56:1063-72.
  4. Mikecz K, et al. 1995. Nat Med. 1:558-63.
  5. Hegde V, et al. 2008. J Leukoc Biol. 84:134-42.
  6. Liu T, et al. 2009. Biol Direct. 4:40.
  7. Santos-Argumedo L, et al. 1997. Immunology. 90:147-53
Gene ID
12505 View all products for this Gene ID
UniProt
View information about CD44 on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 12.19.2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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