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- Clone
- A15073B (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Neutrophil activating protein 2 (NAP2)
- Isotype
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
Recombinant mouse CXCL7 (NAP-2) (Cat. No. 586704) (black circles) induces the chemotaxis of mouse Baf3-mCXCR2 transfectant chemoattractant cells. Ultra-LEAF™ purified anti-mouse CXCL7 (NAP-2) (clone A15073B) (purple squares) neutralizes the chemotaxis of recombinant mouse CXCL7 (NAP-2) (Cat. No. 586704) on mouse Baf3-mCXCR2 cells in a dose-dependent manner whereas the Ultra-LEAF™ purified rat IgG2a, κ isotype control (Cat. No. 400562) (gray triangles) does not have the effect. ND50 range: 0.3 - 2 µg/mL
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 945704 | 1 mg | 1205 CHF | ||||
| 945703 | 100 µg | 358 CHF | ||||
Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.
CXCL7 is an ERL+ chemokine that is secreted by activated platelets, neutrophils, lymphocytes, and macrophages as a precursor. Pro-platelet basic protein or leukocyte derived grow factor (PPBP/LDGF) (a protein of ~14 kD) is modified by post-transcriptional cleavage to give rise to connective tissue activating peptide (CTAP-III), CXCL7, β-thromboglobulin (β-TG), and two variants of thrombocidin (TC-1 and TC-2). CTAP-III is activated by enzymatic proteolysis performed by cell surface-bound cathepsin G (CathG), a chymotryptic serine protease found in primary neutrophil granules. In this way, neutrophils are the mayor cells that convert CTAP-III into CXCL7. In addition, monocytes and mast cells can proteolytically modify CTAP-III to produce CXCL7. In fact, CathG has been detected in mast cells in addition to chymase, another chymotryptic enzyme. Further C-terminal truncations, that eliminate the last four and seven amino acids in CXCL7, generate two additional natural isoforms. These variants are more potent neutrophils activators.
High levels of CTAP-III have been detected in plasma of patients with lung cancer. Because of this, it has been suggested as a biomarker for early lung cancer detection. An increase in positive immunostained cells has been detected in the bronchial submucosa of patients with stable severe chronic obstructive pulmonary disease. In addition, studies with premalignant breast cancer cells transfected with CXCL7 showed that these cells became as invasive as malignant breast cancer cells. CXCL7 also induces stimulation of the lymphangiogenic factors VEGF-C and VEGF-D in human breast cancer cells, suggesting an important role for CXCL7 in tumor invasion.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Recombinant mouse CXCL7
- Formulation
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
- Endotoxin Level
- Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
- Preparation
- The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
- Application
-
Neut - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by neutralization of the chemotaxis induced by recombinant mouse CXCL7 (NAP-2) (Cat. No. 586704) on mouse Baf3-mCXCR2 transfectant chemoattractant cells. ND50 range: 0.3 - 2 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
- RRID
-
AB_2910505 (BioLegend Cat. No. 945704)
AB_2910505 (BioLegend Cat. No. 945703)
Antigen Details
- Structure
- Chemokine
- Distribution
-
Platelets, neutrophils, lymphocytes, macrophages, endothelial cells
- Function
- CXCL7 stimulates chemotaxis and degranulation in neutrophils, induces transendothelial migration and neutrophil adhesion to activated endothelial cells, and has angiogenic activity. LPS induces the precursor in PBMCs
- Interaction
- Neutrophils, keratinocytes
- Ligand/Receptor
- CXCR2
- Biology Area
- Cell Biology, Signal Transduction
- Molecular Family
- Cytokines/Chemokines
- Antigen References
-
- Iida N, et al. 1996. FASEB J. 10:1336-45.
- Schenk BI, et al. 2002. J Immunol. 169:2602-10.
- El-Gedaily A, et al. 2004. J Leukoc Biol. 75:495-503.
- Schiemann F, et al. 2006. Blood. 107:2234-42.
- Tang Z, et al. 2008. Am J Surg. 196:690-6.
- Yee J, et al. 2009. J Clin Oncol. 27:2787-92.
- Yu M, et al. 2010. J Oncol. 2010:939407.
- Lee G, et al. 2011. Am J Transl Res. 3:226-33.
- Nguyen LT and Vogel HJ. 2012. Front Immunol. 3:384.
- Gene ID
- 57349 View all products for this Gene ID
- UniProt
- View information about CXCL7 on UniProt.org
Related Pages & Pathways
Pathways
Related FAQs
- Do you guarantee that your antibodies are totally pathogen free?
-
BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
- Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
-
No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
- Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
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No, BioLegend does not test for pathogens in-house unless otherwise indicated. However, we can recommend an outside vendor to perform this testing as needed.
- Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
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We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.
Other Formats
View All CXCL7 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Ultra-LEAF™ Purified anti-mouse CXCL7 (NAP-2) | A15073B | Neut |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Ultra-LEAF™ Purified anti-mouse CXCL7 (NAP-2)
Recombinant mouse CXCL7 (NAP-2) (Cat. No. 586704) (black cir...
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