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Ultra-LEAF™ Purified anti-mouse TIGIT (Vstm3) Antibody

Pricing & Availability
Clone
4D4/mTIGIT (See other available formats)
Regulatory Status
RUO
Other Names
TIGIT, Vstm3, WUCAM, VSIG9
Isotype
Hamster IgG
Ave. Rating
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Product Citations
publications
4D4slashmTIGIT_ULEAF_TIGIT_Antibody_052819.png
ConA-stimulated (3 days) C57BL/6 mouse splenocytes were stained with CD3 FITC and TIGIT (Vstm3) (clone 4D4/mTIGIT) Ultra-LEAF™ (left) or Armenian Hamster IgG isotype control (right), followed by anti-Armenian hamster IgG PE.
  • 4D4slashmTIGIT_ULEAF_TIGIT_Antibody_052819.png
    ConA-stimulated (3 days) C57BL/6 mouse splenocytes were stained with CD3 FITC and TIGIT (Vstm3) (clone 4D4/mTIGIT) Ultra-LEAF™ (left) or Armenian Hamster IgG isotype control (right), followed by anti-Armenian hamster IgG PE.
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156107 100 µg 264 CHF
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156108 1 mg 688 CHF
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Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.

Description

T cell immunoreceptor with Ig and ITIM domains (TIGIT), also known as V-set and transmembrane domain-containing protein 3 (Vstm3), is a 26 kD, type I transmembrane protein and member of the CD28 family. TIGIT is expressed on activated T cells, follicular T helper, memory, and regulatory T cells as well as on NK cells. Its binding partners include CD155 (PVR) and CD112 (PVRL2). TIGIT is a negative regulator of NK and T cell activation. Engagement of TIGIT by dendritic cells results in their differentiation into a tolerogenic phenotype, with an increased secretion of IL-10 and a diminished production of IL-12. Mice deficient for TIGIT are more susceptible to autoimmune disease.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Hamster
Immunogen
Mouse TIGIT Tetramers
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level
Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

It was reported that this clone can block the interaction between mouse TIGIT and CD155.

Application References

(PubMed link indicates BioLegend citation)
  1. Joller N, et al. 2011. J. Immunol. 186:1338.
RRID
AB_2810422 (BioLegend Cat. No. 156107)
AB_2810422 (BioLegend Cat. No. 156108)

Antigen Details

Structure
Type I transmembrane protein, 26 kD, member of the CD28 family. Contains 1 Ig-like domain and 1 cytoplasmic ITIM motif.
Distribution

Activated T cells, follicular T helper cells, NK, memory T cells, Tregs.

Function
Negative regulator of T cell activation and proliferation. Induction of tolerogenic dendritic cells.
Ligand/Receptor
CD155 (PVR) and CD112 (PVRL2).
Cell Type
Dendritic cells, NK cells, T cells, Tregs
Biology Area
Immunology
Molecular Family
Adhesion Molecules, Immune Checkpoint Receptors
Antigen References
  1. Levin SD, et al. 2011. Eur. J. Immunol. 41:902.
  2. Yu X, et al. 2009. Nat. Immunol. 10:48.
  3. Stanietsky N, et al. 2009. Proc. Natl. Acad. Sci. USA 106:17858
Gene ID
100043314 View all products for this Gene ID
UniProt
View information about TIGIT on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 2    Revision Date: 09.30.2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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