Ultra-LEAF™ Purified anti-human TLR7 Antibody

Pricing & Availability
Clone
S18024F (See other available formats)
Regulatory Status
RUO
Other Names
Toll-like receptor (TLR) 7, CD287
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
S18024F_ULEAF_TLR7_Antibody_042022
Human 293XL-hTLR7 transfected cells were treated with R848 only (red Bar) or R848 plus 1 µg of Ultra-LEAF™ purified rat IgG2a, κ isotype control (yellow bar) or R848 plus 10, 1, 0.1 or 0.01 µg of Ultra-LEAF™ purified anti-human TLR7 (clone S18024F) (purple bars). Supernatant was harvested next day and analyzed using the ELISA MAX™ Deluxe Set Human IL-8 (Cat. No. 431504).
  • S18024F_ULEAF_TLR7_Antibody_042022
    Human 293XL-hTLR7 transfected cells were treated with R848 only (red Bar) or R848 plus 1 µg of Ultra-LEAF™ purified rat IgG2a, κ isotype control (yellow bar) or R848 plus 10, 1, 0.1 or 0.01 µg of Ultra-LEAF™ purified anti-human TLR7 (clone S18024F) (purple bars). Supernatant was harvested next day and analyzed using the ELISA MAX™ Deluxe Set Human IL-8 (Cat. No. 431504).
  • S18024F_ULEAF_TLR7_Antibody_2_042022
    Human peripheral blood plasmacytoid dendritic cells were stained with anti-human CD304 (clone 12C2), fixed and permeabilized using Cyto-Fast™ Fix/Perm Buffer set, and intracellularly stained with anti-human TLR7 (clone S18024F) Ultra-LEAF™ (closed histogram), or purified rat IgG2a, κ isotype control (open histogram) followed by PE anti-rat IgG2a.
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376911 100 µg 270 CHF
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376912 1 mg 743 CHF
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Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.

Description

Toll-like receptor (TLR) 7, also known as CD287 is a type 1 transmembrane protein, member of the TLR receptor family. TLR7 is expressed intracellularly in plasmacytoid dendritic cells, myeloid cells, and B lymphocytes and plays an important role in the innate immune response by binding single-stranded RNA (ssRNA). TLR7 signals through MyD88, which leads to the activation and secretion of IFN-α/β and other inflammatory cytokines.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level
Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

Block - Quality tested
ICFC - Verified

Recommended Usage

Each lot of this antibody is quality control tested by blocking. > 80% blocking was observed with 1 µg antibody per well. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 ug per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone S18024F can be used for blocking R848-activated IL8 cytokine secretion (tested on 293XL/TLR7 cell line) and for intracellular flow cytometry.

RRID
AB_2936679 (BioLegend Cat. No. 376911)
AB_2936679 (BioLegend Cat. No. 376912)

Antigen Details

Structure
Type 1 transmembrane protein, member of the Toll-like receptor (TLR) family
Distribution

Expressed intracellularly in plasmacytoid dendritic cells, myeloid cells, and B lymphocytes, Monocytes

Function
Pattern recognition receptor that detects single-stranded RNA, induces proinflammatory cytokine production
Interaction
B cells, Dendritic cells
Ligand/Receptor
Single-stranded RNA
Cell Type
B cells, Dendritic cells
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules, Innate Immune Signaling
Gene ID
51284 View all products for this Gene ID
UniProt
View information about TLR7 on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 04.20.2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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