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Western-Ready™ Protein Sample Loading Buffer (5X)

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426311 20 mL 47 CHF
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Description

Western-Ready™ Protein Sample Loading Buffer (5X) can be used for the preparation of samples for non-reducing and reducing SDS-PAGE.

Product Details
Technical Data Sheet (pdf)

Product Details

Formulation
300 mM Tris-HCl pH 6.8, 10% Sodium dodecyl sulfate, 30% glycerol, 0.02% Bromophenol blue
Storage & Handling
Ambient room temperature
Application

WB - Quality tested
Recommended Usage

Dilute Western-Ready™ Protein Sample Loading Buffer (5X) to a 1X concentration (1:4 by volume) using appropriate amount of sample and diluting solution (ie: water, lysis buffer, etc). For sample that are to be used for reducing PAGE, a reducing agent such as as β-ME, DTT, or TCEP must be added to buffer prior to mixing and heating sample.

Application Notes

Western-Ready™ Protein Sample Loading Buffer (5X) contains SDS, which may precipitate at cooler temperatures. If preceipitates are observed in the buffer, heat in 37°C water bath for 5 min to bring SDS back into solution.

Western-Ready™ Protein Sample Loading Buffer (5X) is compatible with Bis-Tris, Tris-Glycine, and Tris-Acetate gels.

Western-Ready™ Protein Sample Loading Buffer (5X) does not include any reducing agents. For reducing SDS-PAGE, we recommend adding a reducing agent such as ß-ME, DTT, TCEP to buffer prior to mixing with sample.

This buffer should not be used for native PAGE.

This buffer may be used to elute captured immune complexes during immunoprecipitation-based applications.

Antigen Details

Gene ID
NA

Related FAQs

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Go To Top Version: 1    Revision Date: 11.19.2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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