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Alexa Fluor® 700 anti-mouse CD48 Antibody

Pricing & Availability
Clone
HM48-1 (See other available formats)
Regulatory Status
RUO
Other Names
BCM1, SLAMF2, Blast-1
Isotype
Armenian Hamster IgG
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Product Citations
publications
HM48-1_AF700_061510
C57BL/6 splenocytes stained with HM48-1 Alexa Fluor® 700
  • HM48-1_AF700_061510
    C57BL/6 splenocytes stained with HM48-1 Alexa Fluor® 700
Compare all formats See Alexa Fluor® 700 spectral data
Cat # Size Price Quantity Check Availability Save
103425 25 µg 94 CHF
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103426 100 µg 215 CHF
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Description

CD48 is a 45 kD GPI-anchored glycoprotein also known as BCM1, Blast-1 (human), and OX-45 (rat). It is a member of the Ig superfamily, expressed on T and B cells and monocytes/macrophages. It plays a role in adhesion and T cell recognition. The primary ligands for CD48 are CD2 and CD244.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
Mouse T lymphoma MBL-2
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 700 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is highly recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 700 has a maximum emission of 719 nm when it is excited at 633 nm / 635 nm. Prior to using Alexa Fluor® 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

The HM48-1 antibody is useful for blocking in vitro and in vivo CD48 mediated interactions. Additional reported applications (for the relevant formats) include: immunoprecipitation1, costimulation of T cell proliferation1,2, blocking of CD48-CD2 interaction1, and inhibition of CTL activity and graft rejection1,2. For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. Nos. 103452-103456) with a lower endotoxin limit (Endotoxin < 0.01 EU/µg).

Application References

(PubMed link indicates BioLegend citation)
  1. Kato K, et al. 1992. J. Exp. Med. 176:1241. (IP, Costim, Block)
  2. Qin L, et al. 1994. J. Exp. Med. 179:341. (Costim, Block)
Product Citations
  1. Upadhaya S, et al. 2020. Cell Stem Cell. 27(2):336-345.e4. PubMed
  2. Luchsinger LL, et al. 2020. Cell Stem Cell. 25(2):225-240. PubMed
  3. Dickerson KM, et al. 2022. Blood Cancer Discov. 3:240. PubMed
  4. Li X, et al. 2022. Cell. 185:1709. PubMed
  5. Meacham CE, et al. 2022. Nat Cell Biol. 24:697. PubMed
  6. Rundberg Nilsson A, et al. 2023. iScience. 26:106341. PubMed
  7. Hirata Y et al. 2018. Cell stem cell. 22(3):445-453 . PubMed
  8. Schloss MJ, et al. 2022. Nat Immunol. 23:605. PubMed
  9. Riether C, et al. 2021. Cell Reports. 34(4):108663. PubMed
  10. Nakamura‐Ishizu A et al. 2018. Cell reports. 25(7):1772-1785 . PubMed
  11. McAlpine CS, et al. 2021. Nature. 595:701. PubMed
  12. Comazzetto S et al. 2018. Cell stem cell. 24(3):477-486 . PubMed
  13. Hinge A, et al. 2020. Cell Stem Cell. 26(3):420-430. PubMed
  14. Frodermann V, et al. 2019. Nat Med. 25:1761. PubMed
  15. de Almeida MJ et al. 2017. Cell stem cell. 21(6):725-729 . PubMed
  16. Tummers B, et al. 2020. Immunity. 52(6):994-1006.e8. PubMed
  17. Heil J, et al. 2021. Nat Commun. 12:6963. PubMed
  18. Rai S, et al. 2022. Nat Commun. 13:5346. PubMed
  19. Li K, et al. 2020. Nat Commun. 0.795138889. PubMed
  20. Yamashita M, et al. 2019. Cell Stem Cell. 25:357. PubMed
  21. Säwen P et al. 2018. eLife. 7 pii: e41258. PubMed
  22. Chavez JS, et al. 2022. Cells. 11:. PubMed
  23. Heyde A, et al. 2021. Cell. 184(5):1348-1361.e22. PubMed
  24. Wünsche P et al. 2018. Cell stem cell. 23(1):132-146 . PubMed
  25. Fast EM, et al. 2021. Elife. 10:. PubMed
  26. Young K, et al. 2021. Cell Stem Cell. . PubMed
  27. Lv K, et al. 2021. Cell Stem Cell. 28(7):1275-1290.e9. PubMed
  28. Li CY, et al. 2022. Int J Mol Sci. 23:. PubMed
  29. Gu Z, et al. 2021. Nat Genet. 53:672. PubMed
RRID
AB_10612755 (BioLegend Cat. No. 103425)
AB_10612755 (BioLegend Cat. No. 103426)

Antigen Details

Structure
Ig superfamily, 45 kD
Distribution

T cells and B cells, monocytes/macrophages

Function
Adhesion, T cell recognition
Ligand/Receptor
CD2, CD244
Cell Type
B cells, Macrophages, Monocytes, T cells
Biology Area
Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Flament C, et al. 1996. Hum. Immunol. 46:82.
3. Van der Merwe PA, et al. 1995. Curr. Biol. 5:74.
4. Latchman Y, et al. 1998. J. Immunol. 161:5809.

Gene ID
12506 View all products for this Gene ID
UniProt
View information about CD48 on UniProt.org
Go To Top Version: 2    Revision Date: 06.27.2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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