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Anti-DYKDDDDK Tag (L5) Affinity Gel

Pricing & Availability
Clone
L5 (See other available formats)
Regulatory Status
RUO
Other Names
FLAG tag
Isotype
Rat IgG2a, λ
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Product Citations
publications
L5_gel_graph_113011
Anti-DYKDDDDK tag affinity resin (L5-agarose) retains binding capacity even after 10 rounds of purification and elution.
  • L5_gel_graph_113011
    Anti-DYKDDDDK tag affinity resin (L5-agarose) retains binding capacity even after 10 rounds of purification and elution.
  • pos2_L5_gel_113011
    Anti-DYKDDDDK tag affinity resin (L5-agarose) retains binding capacity even after 10 rounds of purification and elution.
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Cat # Size Price Quantity Check Availability Save
651501 500 µL 206 CHF
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651502 2 mL 452 CHF
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651503 5 mL 815 CHF
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Description

The DYKDDDDK tag, commonly referred to as Sigma®'s FLAG® Tag, is often used as a protein modification in order to simplify the labeling and detection of proteins.This unique amino acid sequence allows for specific antibody detection in western blotting, immunoprecipitation, and immunostaining techniques.Due to the short sequence, this modification is not likely to affect the structure or function of the modified proteins.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Epitope tag
Reported Reactivity
Species independent
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
DYKDDDDK-tagged mouse Langerin
Formulation
50% anti-DYKDDDDK-tag (L5) antibody conjugated resin is supplied in 1X PBS and 0.09% NaN3. The volume specified for each catalog number indicates the volume of resin included.
Preparation
The antibody was purified by affinity chromatography.
Storage & Handling
Upon receipt, store between 2°C and 8°C. The unopened product is stable for one year upon arrival.
Application

IP, Purification of DYKDDDDK-tagged fusion proteins from cell lysates - Quality tested
 
Recommended Usage

Resin binding capacity is greater than 0.8 mg/mL.

Application Notes

The L5 clone has been demonstrated to have 2-8 fold better sensitivity in WB than another commonly used antibody clone, M2.

Application References

(PubMed link indicates BioLegend citation)
  1. Park SH, et al. 2008. J Immunol Methods. 331:27.
  2. Moon SH, et al. 2010. J. Biol Chem. 285:12935. PubMed
  3. Sasaki M, et al. 2011. J. Biol Chem. 286:39370. PubMed
  4. Sonder SU, et al. 2012. J Immunol. 188:5906. PubMed
  5. Jiang Y, et al. 2013. Int Immunol. 25:235. PubMed
  6. Zuo X, et al. 2014. PLoS One. 9:84748. PubMed
  7. Toyo-Oak K, et al. 2014. J Neurosci. 34:12168. PubMed
Product Citations
  1. Basavarajappa SC, et al. 2022. iScience. 25:104716. PubMed
  2. Wang W, et al. 2022. Cell Rep. 41:111582. PubMed
  3. Li W, et al. 2023. Life Sci Alliance. 6: . PubMed
  4. Dai J, et al. 2022. Nat Cancer. 3:25. PubMed
  5. Vervliet T, et al. 2015. Biochem Biophys Res Commun. 460:291. PubMed
  6. Mamedov T, et al. 2021. Vaccines (Basel). 9:. PubMed
  7. Mamedov T, et al. 2017. PLoS One.. 10.1371/journal.pone.0183589. PubMed
  8. Meng KP, et al. 2020. J Exp Med. 217:00:00. PubMed
  9. Raman V, et al. 2021. Nat Commun. 12:6116. PubMed
  10. Grimley R, et al. 2012. PLoS One. 7:e39847. PubMed
  11. Mamedov T, et al. 2021. Viruses. 13:. PubMed
  12. Qian X, et al. 2019. Molecular Cell. 76(6):885-895.e7. PubMed
  13. Qian X et al. 2017. Molecular cell. 65(5):917-931 . PubMed
  14. Li X, et al. 2020. Cell Rep. 33:108563. PubMed
  15. Xie C, et al. 2022. Cell Death Dis. 13:701. PubMed
RRID
AB_10962945 (BioLegend Cat. No. 651501)
AB_10959657 (BioLegend Cat. No. 651502)
AB_10962692 (BioLegend Cat. No. 651503)

Antigen Details

Biology Area
Cell Biology
Antigen References

1. Einhauer A. 2001. J. Biochem. Biophys. Methods. 49:455.
2. Knappik A and Pluckthun A. 1994. Biotechniques. 17:754.

Gene ID
NA
UniProt
View information about DYKDDDDK on UniProt.org

Related FAQs

Does a relatively low pH such as 4.5 dissociate the L5 antibody from the resin?
pH4.5 should not diassociate the antibody from the resin. But this pH could affect the binding of the FLAG-tagged target to the L5 antibody.
Go To Top Version: 3    Revision Date: 09.21.2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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