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Anti-Neurofilament Marker (pan-neuronal, cocktail) Antibody (Previously Covance catalog# SMI-311R)

Pricing & Availability
Clone
SMI 311 (See other available formats)
Regulatory Status
RUO
Other Names
Neurofilament Marker
Previously
Covance Catalog# SMI-311R
Isotype
Mouse IgG1/Mouse IgM
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Product Citations
publications
A-SMI-311_Neutrofilament_Marker_Antibody_1_040319
IHC staining of anti-Neurofilament Marker (pan-neuronal, cocktail) antibody (clone SMI 311) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Retrieve-All Antigen Unmasking System 3: Acidic, 100X (Cat. No. 927601), the tissue was incubated with a 1:5000 dilution of the primary antibody overnight at 4°C. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • A-SMI-311_Neutrofilament_Marker_Antibody_1_040319
    IHC staining of anti-Neurofilament Marker (pan-neuronal, cocktail) antibody (clone SMI 311) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Retrieve-All Antigen Unmasking System 3: Acidic, 100X (Cat. No. 927601), the tissue was incubated with a 1:5000 dilution of the primary antibody overnight at 4°C. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • B-SMI-311_Neutrofilament_Marker_Antibody_2_040319
    IHC staining of anti-Neurofilament Marker (pan-neuronal, cocktail) antibody (clone SMI 311) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Retrieve-All Antigen Unmasking System 3: Acidic, 100X (Cat. No. 927601), the tissue was incubated with a 1:5000 dilution of the primary antibody overnight at 4°C. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • C-SMI-311_Neutrofilament_Marker_Antibody_3_040319
    IHC staining of anti-Neurofilament Marker (pan-neuronal, cocktail) antibody (clone SMI 311) on formalin-fixed paraffin-embedded human brain tissue. Following antigen retrieval using Retrieve-All Antigen Unmasking System 3: Acidic, 100X (Cat. No. 927601), the tissue was incubated with a 1:5000 dilution of the primary antibody overnight at 4°C. BioLegend's Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • D-SMI-311_Neutrofilament_Marker_Antibody_5_042919
    IHC staining of anti-Neurofilament Marker (pan-neuronal, cocktail) antibody (clone SMI 311) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Retrieve-All Antigen Unmasking System 3: Acidic, 100X (Cat. No. 927601), the tissue was incubated with a 1:5000 dilution of the primary antibody overnight at 4°C, followed by incubation with Alexa Fluor® 647 Goat anti-mouse IgG (Cat. No. 405322) for one hour at room temperature. Nuclei were counterstained with DAPI, and the slide was mounted with ProLong™ Gold Antifade Mountant. The image was captured with a 40X objective. Scale Bar: 50 µm
  • E-SMI-311_Neutrofilament_Marker_Antibody_6_042919
    IHC staining of anti-Neurofilament Marker (pan-neuronal, cocktail) antibody (clone SMI 311) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Retrieve-All Antigen Unmasking System 3: Acidic, 100X (Cat. No. 927601), the tissue was incubated with a 1:5000 dilution of the primary antibody overnight at 4°C, followed by incubation with Alexa Fluor® 647 Goat anti-mouse IgG (Cat. No. 405322) for one hour at room temperature. Nuclei were counterstained with DAPI, and the slide was mounted with ProLong™ Gold Antifade Mountant. The image was captured with a 40X objective. Scale Bar: 50 µm
  • F-SMI-311_Neutrofilament_Marker_Antibody_4_040319
    Western blot of anti-Neurofilament Marker (pan-neuronal, cocktail) antibody (clone SMI 311). Lane 1: Molecular weight marker; Lane 2: 30 µg of human brain lysate; Lane 3: 30 µg of mouse brain lysate; Lane 4: 30 µg of rat brain lysate. The blot was incubated with a 1:10,000 dilution of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
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837801 100 µL 375 CHF
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837802 500 µL 673 CHF
 
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Description

Neurofilaments (NF) are approximately 10 nanometer intermediate filaments found in neurons. They are a major component of the neuronal cytoskeleton and their function is primarily to provide structural support for the axon and to regulate axon diameter. There are three major NF subunits, and the names given to these subunits are based upon the apparent molecular mass of the mammalian subunits on SDS-PAGE. The light or lowest (NF-L) runs at 68-70 kD, the medium or middle (NF-M) runs at about 145-160 kD, and the heavy or highest (NF-H) runs at 200-220 kD. However, the actual molecular weight of these proteins is considerably lower due to the highly charged C-terminal regions of the molecules. The level of NF gene expression correlates with the axonal diameter, which controls how fast electrical signals travel down the axon. Mutant mice with NF abnormalities have phenotypes resembling amyotrophic lateral sclerosis. NF immunostaining is common in diagnostic neuropathology. It is useful for differentiating neurons (positive for NF) from glia (negative for NF).

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Ascites Fluid (contains 0.01M sodium azide).
Preparation
Ascites
Concentration
The concentration is not quantified as this product is sold as undiluted crude mouse ascites fluid. The concentration might vary from lot-to-lot and an estimated concentration would be 1-3 mg/ml.
Storage & Handling
Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody. For long-term storage, keep the antibody at -80°C.
Application

IHC-P - Quality tested
WB - Verified
IHC-F, ICC - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a dilution range of 1:1000 - 1:5000 is suggested. For Western blotting, the suggested use of this reagent is 1:5000 - 1:10000 per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

SMI 311 has been selected to provide a specific marker for neurons in tissue sections and cultures. In contrast to individual antinonphosphoneurofilaments that identify different subset of neurons and are, therefore, especially suitable for defining anatomic and functional differences in normal and pathologic neurons, SMI 311 is a convenient marker for neurons in general and their differentiation from non-neuronal cells. SMI 311 provides an early marker of neuronal migration and differentiation in human fetal development yielding Golgi-like images without the disadvantages of lack of selectivity and poor specificity of the Golgi technique. In specifically delineating cell bodies and dendrites, SMI 311 has been used to trace the "inside-out gradient" of neuron production and differentiation. Pathologic conditions, such as undernutrition affect SMI 311-visualized soma size and dendritic arborization.

Application References

(PubMed link indicates BioLegend citation)
  1. Tsunoda I, Kuang LQ, Libbey JE, Fujinami RS. Axonal injury heralds virus-induced demyelination. Am J Path 162:1259-1269, 2003. (IHC-P)
  2. Ulfig N, Nickel J, Bohl J. Monoclonal antibodies SMI 311 and SMI 312 as tools to investigate the maturation of nerve cells and axonal patterns in human fetal brain. Cell Tissue Res 291:433, 1998.
  3. Frola E, et al. 2013. PLoS One 2:e56311. (IHC-F)
  4. Ivanovic A, et al. 2012. J. Cell Biol. 3:337. (IHC-F, WB) PubMed
  5. Petzold A, et al. 2011. Brain 134:464. (IHC-P, WB) PubMed
  6. Del Valle L,et al. Cancer Biol Ther.9:286. (IHC-P, ICC, WB) PubMed
Product Citations
  1. Nguyen LH, et al. 2022. Brain. 145:1310. PubMed
  2. Batiuk MY, et al. 2022. Sci Adv. 8:eabn8367. PubMed
  3. Yang J, et al. 2023. Brain Sci. 13:. PubMed
  4. Checri R, et al. 2023. Brain Commun. 5:fcad174. PubMed
  5. Hsieh L, et al. 2016. Nat Commun. 7:11753. PubMed
  6. Ivanovic A, et al. 2012. J Cell Biol. 196:337-344. PubMed
  7. Baldassari S, et al. 2019. Acta Neuropathol. 138:885. PubMed
  8. Pallebage–Gamarallage M, et al. 2018. BMC Neurosci. 19:11. PubMed
  9. Needham BD, et al. 2022. Nature. 602:647. PubMed
  10. Barr JL, et al. 2021. Cells. 10:. PubMed
  11. Lee WS, et al. 2019. Ann Clin Transl Neurol. 6:1338. PubMed
  12. Valle L, et al. 2010. Cancer Biol Ther. 9:286-294. PubMed
  13. del Blanco B, et al. 2019. Cell Death Differ. 26:2208. PubMed
  14. Zub E, et al. 2019. FASEB J. 33:13998. PubMed
  15. Petzold A, et al. 2011. Brain. 134:464-483. PubMed
  16. Xia D, et al. 2022. Mol Neurodegener. 17:41. PubMed
  17. Hudry E, et al. 2019. Life Sci Alliance. 2:. PubMed
RRID
AB_2565384 (BioLegend Cat. No. 837801)
AB_2565384 (BioLegend Cat. No. 837802)

Antigen Details

Structure
Expected MW: medium or middle NF (NF-M) runs at 145-160 kD, and the heavy or highest NF (NF-H) runs at 200-220 kD
Cell Type
Mature Neurons
Biology Area
Cell Biology, Neuroscience, Neuroscience Cell Markers
Molecular Family
Intermediate Filaments
Gene ID
4747 View all products for this Gene ID 4744 View all products for this Gene ID 4741 View all products for this Gene ID
UniProt
View information about Neurofilament Marker on UniProt.org
Go To Top Version: 3    Revision Date: 08.29.2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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