Loading...

APC anti-mouse IL-17A Antibody

Pricing & Availability
Clone
TC11-18H10.1 (See other available formats)
Regulatory Status
RUO
Other Names
Interleukin-17, Cytotoxic T lymphocyte-associated antigen 8 (CTLA-8)
Isotype
Rat IgG1, κ
Ave. Rating
Submit a Review
Product Citations
publications
TC11-18H10_APC_031808
PMA (20 ng/ml) + ionomycin (1 µg/ml) -stimulated (6 hours + monensin, 2 µM) mouse thymoma cell line EL-4 intracellularly stained with TC11-18H10.1 APC
  • TC11-18H10_APC_031808
    PMA (20 ng/ml) + ionomycin (1 µg/ml) -stimulated (6 hours + monensin, 2 µM) mouse thymoma cell line EL-4 intracellularly stained with TC11-18H10.1 APC
Compare all formats See APC spectral data
Cat # Size Price Quantity Check Availability Save
506915 25 µg 131 CHF
Check Availability


Need larger quantities of this item?
Request Bulk Quote
506916 100 µg 296 CHF
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

IL-17, also known as CTLA-8, is a T cell-expressed pleiotropic cytokine that exhibits a high degree of homology to a protein encoded by the ORF13 gene of herpes virus Saimiri. IL-17 is produced by Th cells (Th17) that are distinct from the traditional Th1- and Th2-cell subsets. IL-23 plays an important role in triggering IL-17 production. Both recombinant and natural IL-17 have been shown to exist as disulfide linked homodimers. IL-17 exhibits multiple biological activities on a variety of cells including: the induction of IL-6 and IL-8 production in fibroblasts, activation of NF-κB, and costimulation of T cell proliferation. IL-17 is an essential inflammatory mediator in the development of autoimmune diseases. Neutralization of IL-17 with monoclonal antibody is able to ameliorate the disease course.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
E. coli expressed, recombinant mouse IL-17A
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The IL-17A antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

ELISA Capture3,4 and ELISPOT Capture5: The purified TC11-18H10.1 antibody is useful as the capture antibody in a sandwich ELISA, when used in conjunction with the biotinylated TC11-8H4 antibody (Cat. No. 507002) as the detecting antibody and recombinant mouse IL-17 (Cat. No. 576009) as the standard.
Flow Cytometry2-4,7,8,11,12: The TC11-18H10.1 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IL-17-producing cells within mixed cell populations.
Neutralization6,9: The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for neutralization of mouse IL-17 bioactivity in vivo and in vitro (Cat. No. 506906).

Application References

(PubMed link indicates BioLegend citation)
  1. Kennedy J, et al. 1996. J. Interferon Cytokine Res. 16:611.
  2. Schubert D, et al. 2004. J. Immunol. 172:4503. (ICFC)
  3. Infante-Duarte C, et al. 2000. J. Immunol. 165:6107. (ICFC, ELISA Capture)
  4. Harrington LE, et al. 2005. Nature Immunol. doi:10.1038/ni1254. (ICFC, ELISA Capture)
  5. Nekrasova T, et al. 2005. J. Immunol. 175:2734. (ELISPOT Capture)
  6. Yen D, et al. 2006. J. Clin. Invest. 116:1310. (Neut)
  7. Ehirchiou D, et al. 2007. J. Exp. Med. 204:1519. (ICFC)
  8. Kang SG, et al. 2007. J. Immunol. 179:3724. (ICFC)
  9. Smith E, et al. 2008. J. Immunol. 181:1357. (Neut) PubMed
  10. Neufert C, et al. 2007. Eur. J. Immunol. 37:1809. PubMed
  11. Wang C, et al. 2009. Mucosal Immunol 2:173. (ICFC) PubMed
  12. Cui Y, et al. 2009. Invest. Ophth. Vis. Sci. 50:5811. (ICFC) PubMed
  13. Kivisäkk P, et al. 2009. Ann. Neurol. 65:457. PubMed
  14. Cooney LA, et al. 2011. J. Immunol. 187:4440. PubMed
  15. Ma Y, et al. 2012. PLoS One. 7:e40763. PubMed
  16. Murakami R, et al. 2013. PLoS One. 8:73270. PubMed
Product Citations
  1. Liu J, et al. 2019. Immunity. 50:600. PubMed
  2. Lebson L, et al. 2010. J Immunol. 185:7161. PubMed
  3. Garo LP, et al. 2021. Nat Commun. 12:2419. PubMed
  4. Marchelletta RR, et al. 2021. J Clin Invest. 131:. PubMed
  5. Su Y, et al. 2022. J Hematol Oncol. 15:99. PubMed
  6. Miki H, et al. 2020. J Immunol. 204:1892. PubMed
  7. Zutshi S, et al. 2021. Immunology. 163:460. PubMed
  8. Yang L, et al. 2022. Acta Pharmacol Sin. 43:963. PubMed
  9. Wang YC, et al. 2023. J Exp Med. 220:. PubMed
  10. Schwarz A, et al. 2023. Front Immunol. 14:1038689. PubMed
  11. Gerber AN, et al. 2021. Cell Rep. 37:109816. PubMed
  12. Kenichi Shimada et al. 2018. Immunity. 49(5):873-885 . PubMed
  13. Manivasagam S, et al. 2022. J Immunol. 208:1341. PubMed
  14. MaruYama T 2015. Biochem Biophys Res Commun. 464: 586-589. PubMed
  15. Yang W, et al. 2020. Nat Commun. 3.553472222. PubMed
  16. Periasamy S, et al. 2017. Nat Commun. 8:15564. PubMed
  17. Andreas N, et al. 2021. Arthritis Res Ther. 23:222. PubMed
  18. Ogawa C et al. 2018. Cell reports. 25(1):19-28 . PubMed
  19. Zhong J, et al. 2018. Sci Adv. 4:eaas9864. PubMed
  20. Li J, et al. 2010. Blood. 116:5615. PubMed
  21. Hering L, et al. 2021. Int J Mol Sci. 22:. PubMed
  22. Cai B, et al. 2021. Mol Cancer. 20:165. PubMed
  23. Zhou HF, et al. 2022. Front Pharmacol. 13:778755. PubMed
  24. Yang W, et al. 2020. Mucosal Immunol. 13:788. PubMed
  25. Wang Z, et al. 2020. Autophagy. 1:. PubMed
  26. Sasaki K, et al. 2019. Nat Commun. 10:3878. PubMed
  27. Sato Y, et al. 2021. J Clin Invest. Online ahead of print. PubMed
  28. Ontiveros-Padilla L, et al. 2021. Microorganisms. 9:. PubMed
  29. Mishra P, et al. 2013. Brain Behav Immun. 34:98. PubMed
  30. Ma C, et al. 2016. Nature. 531:253-257. PubMed
  31. Koren N, et al. 2021. Cell Host Microbe. 29(2):197-209.e5. PubMed
  32. Zongyi Y, et al. 2017. PLoS One. 10.1371/journal.pone.0189617. PubMed
  33. Xiong Y, et al. 2018. Int J Clin Exp Pathol. 3.803472222. PubMed
  34. He J, et al. 2020. Cell Reports. 29(9):2718-2730.e6.. PubMed
  35. Zhao F, et al. 2022. Front Immunol. 13:873720. PubMed
  36. Shami A, et al. 2015. Sci Rep. 5: 13904. PubMed
  37. Shiomi A, et al. 2014. J Immunol. 193:849. PubMed
  38. Pedraza–Zamora CP, et al. 2017. Parasite Immunol. 39:4. PubMed
  39. Yin Q, et al. 2021. Proc Natl Acad Sci U S A. 118: . PubMed
  40. Wu H, et al. 2012. J Am Soc Nephrol. 23:1701. PubMed
  41. Chuang YM, et al. 2018. Front Cell Infect Microbiol. 8:31. PubMed
  42. Lee CG, et al. 2019. JCI Insight. 4:5. PubMed
  43. Doonan J, et al. 2019. Nat Commun. 10:1554. PubMed
  44. Liao T, et al. 2017. Front Immunol. 8:1334. PubMed
  45. Bonnefoy F, et al. 2011. J Immunol. 186:5696. PubMed
  46. Dai G, et al. 2021. mSphere. 6: . PubMed
  47. Du Y, et al. 2022. Nat Commun. 13:231. PubMed
  48. Garo LP, et al. 2019. Cell Rep. 28:3353. PubMed
  49. Bankoti R, et al. 2017. Sci Rep. 10.1038/s41598-017-12171-3. PubMed
  50. Wei W, et al. 2022. mSystems. 7:e0046922. PubMed
  51. Zanvit P, et al. 2015. Nat Commun. 6: 8424. PubMed
  52. Tian X, et al. 2022. J Cell Mol Med. 26:693. PubMed
  53. Liu B, et al. 2022. Mol Med Rep. 26:. PubMed
  54. Yan J, et al. 2020. Cell Rep. 107820:31. PubMed
  55. Arbelaez C, et al. 2015. J Immunol. 195: 1974-1983. PubMed
  56. Kastelberg B, et al. 2020. PLoS Pathog. 16:e1008854. PubMed
  57. Jin C, et al. 2019. Cell. 176:998. PubMed
  58. Kyburz A, et al. 2019. J Allergy Clin Immunol. 143:1496. PubMed
  59. Han F, et al. 2020. Invest Ophthalmol Vis Sci. 61:24. PubMed
  60. Timilshina M, et al. 2017. PLoS One. 10.1371/journal.pone.0168942. PubMed
  61. Yang BH, et al. 2020. Cell Reports. 27(12):3629-3645.e6.. PubMed
  62. Ding Y, et al. 2020. Evid Based Complement Alternat Med. 2020:9521073. PubMed
  63. Lou Y, et al. 2021. Int J Mol Sci. 22:. PubMed
  64. Wu B, et al. 2021. Immunity. 54(2):308-323.e6. PubMed
  65. Cabrera-Perez C, et al. 2015. J Immunol . 194:1609-20. PubMed
  66. Robb R, et al. 2011. Blood. 118:3399. PubMed
  67. Yuan X, et al. 2017. Elife. 6:e29540. PubMed
  68. Meyer Zu Horste G et al. 2018. Immunity. 48(3):556-569 . PubMed
  69. Wang Y, et al. 2021. Nat Commun. 12:4964. PubMed
  70. Callejas BE, et al. 2021. Front Immunol. 12:744738. PubMed
  71. Zhang H, et al. 2017. Immunol Cell Biol. 10.1038/icb.2017.11. PubMed
  72. Roy S, et al. 2021. Nat Commun. 12:3182. PubMed
  73. Makino A, et al. 2021. iScience. 24:103201. PubMed
  74. Kiuchi M, et al. 2021. J Exp Med. 218:. PubMed
  75. Dallari S, et al. 2021. Cell Host Microbe. 29(6):1014-1029.e8. PubMed
  76. Xu Y, et al. 2016. Nat Commun. 7:12073. PubMed
  77. Webb LM, et al. 2020. J Clin Invest. 130:1683. PubMed
  78. Okuyama Y, et al. 2020. FASEB J. 540:34. PubMed
  79. Miranda PM, et al. 2018. Microbiome. 6:57. PubMed
  80. Wang Y, et al. 2021. Front Immunol. 11:601601. PubMed
  81. Stevanin M, et al. 2017. Eur J Immunol. 10.1002/eji.201646565. PubMed
  82. Yu H, et al. 2015. PLoS One. 10: 0143001. PubMed
  83. Ramanan D, et al. 2020. Cell. 181(6):1276-1290. PubMed
  84. Liu YW, et al. 2022. JCI Insight. 7:. PubMed
  85. Chen YL, et al. 2022. Front Neurosci. 16:876582. PubMed
  86. Yue T, et al. 2022. Cell Death Differ. 29:218. PubMed
  87. Zhang X, et al. 2021. Front Pharmacol. 12:706748. PubMed
  88. Deng J, et al. 2021. EBioMedicine. 70:103505. PubMed
  89. Timilshina M, et al. 2020. Cell Reports. 27(10):2948-2961.e7.. PubMed
  90. Lytle NK et al. 2019. Cell. 177(3):572-586 . PubMed
  91. Long L, et al. 2021. Nature. 600:308. PubMed
  92. Nagashima H, et al. 2016. J Immunol. 196: 4082 - 4089. PubMed
  93. Chen S, et al. 2018. Nat Commun. 9:5298. PubMed
  94. Bing Wu et al. 2018. Immunity. 49(5):886-898 . PubMed
RRID
AB_536017 (BioLegend Cat. No. 506915)
AB_536017 (BioLegend Cat. No. 506916)

Antigen Details

Structure
Cytokine; dimer; 15 kD (Mammalian).
Bioactivity
Secretion of IL-6, IL-8, G-CSF, prostaglandin E2 by epithelial, endothelial or fibroblastic cells; stimulates cell migration, cord formation, and IL-6 secretion by stromal cells
Cell Sources
CD4+ memory T cells
Cell Targets
Fibroblasts, epithelial and endothelial cells, stromal cells
Receptors
IL-17R (CD217)
Biology Area
Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
Cytokines/Chemokines
Antigen References

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. Numasaki M, et al. 2002. Blood 101:2620.
3. Fossiez F, et al. 1996. J. Exp. Med. 183:2593.
4. Yao Z, et al. 1997. Cytokine 9:794.
5. Dong C. 2006. Nat. Rev. Immunol. 6:329.
6. Hofstetter HH, et al. 2005 Cell. Immunol. 237:123.

Gene ID
16171 View all products for this Gene ID
UniProt
View information about IL-17A on UniProt.org

Related FAQs

Does the anti-mouse IL-17 antibody (clone TC11-18H10.1) recognize the A or F isoform?
Clone TC11-18H10.1 recognizes IL-17A isoform, but it also recognizes the IL-17A/F heterodimer via IL-17A binding.

Other Formats

View All IL-17A Reagents Request Custom Conjugation
Description Clone Applications
PE anti-mouse IL-17A TC11-18H10.1 ICFC
Purified anti-mouse IL-17A TC11-18H10.1 ELISA Capture,CyTOF®,ELISPOT Capture,ICFC
FITC anti-mouse IL-17A TC11-18H10.1 ICFC
Alexa Fluor® 488 anti-mouse IL-17A TC11-18H10.1 ICFC
Alexa Fluor® 647 anti-mouse IL-17A TC11-18H10.1 ICFC
Alexa Fluor® 700 anti-mouse IL-17A TC11-18H10.1 ICFC
APC anti-mouse IL-17A TC11-18H10.1 ICFC
Pacific Blue™ anti-mouse IL-17A TC11-18H10.1 ICFC
PerCP/Cyanine5.5 anti-mouse IL-17A TC11-18H10.1 ICFC
PE/Cyanine7 anti-mouse IL-17A TC11-18H10.1 ICFC
Brilliant Violet 421™ anti-mouse IL-17A TC11-18H10.1 ICFC
Brilliant Violet 605™ anti-mouse IL-17A TC11-18H10.1 ICFC
Brilliant Violet 650™ anti-mouse IL-17A TC11-18H10.1 ICFC
Brilliant Violet 785™ anti-mouse IL-17A TC11-18H10.1 ICFC
Brilliant Violet 510™ anti-mouse IL-17A TC11-18H10.1 ICFC
Purified anti-mouse IL-17A (Maxpar® Ready) TC11-18H10.1 ELISA Capture,CyTOF®
PE/Dazzle™ 594 anti-mouse IL-17A TC11-18H10.1 ICFC
APC/Cyanine7 anti-mouse IL-17A TC11-18H10.1 ICFC
Brilliant Violet 711™ anti-mouse IL-17A TC11-18H10.1 ICFC
PerCP anti-mouse IL-17A TC11-18H10.1 ICFC
Ultra-LEAF™ Purified anti-mouse IL-17A TC11-18H10.1 ELISA Capture,CyTOF®,ELISPOT Capture,Neut,ICFC
PE/Cyanine5 anti-mouse IL-17A TC11-18H10.1 ICFC
Go To Top Version: 2    Revision Date: 10.16.2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account