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APC anti-mouse Perforin Antibody

Pricing & Availability
Clone
S16009A (See other available formats)
Regulatory Status
RUO
Other Names
Prf1, Pfp, Pfn, Pfn-1
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
S16009A_APC_Perforin_Antibody_1_111617
C57BL/6 splenocytes were stained with CD49b PE. Cells were fixed, permeabilized, and stained with APC anti-mouse Perforin (clone S16009A, top) or APC rat IgG2a, κ isotype control (bottom).
  • S16009A_APC_Perforin_Antibody_1_111617
    C57BL/6 splenocytes were stained with CD49b PE. Cells were fixed, permeabilized, and stained with APC anti-mouse Perforin (clone S16009A, top) or APC rat IgG2a, κ isotype control (bottom).
  • S16009A_APC_Perforin_Antibody_2_111617
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154303 25 µg 149 CHF
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154304 100 µg 374 CHF
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Description

Perforin is a 70 kD cytolytic protein that is expressed in the cytoplasmic granules of cytotoxic T lymphocytes (CTLs) and natural killer (NK) cells. Perforin is one of the major effector molecules used by cytotoxic T cells and NK cells to mediate targeted cell lysis.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse perforin transfected cells.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

Clone S16009A reacts with perforin from both C57BL/6 and BALB/c strains.

Product Citations
  1. Saxena V, et al. 2022. Cell Rep. 39:110727. PubMed
  2. Cai Z, et al. 2023. Adv Sci (Weinh). 10:e2207155. PubMed
  3. Wang F, et al. 2023. Int J Mol Sci. 24:. PubMed
  4. Jiang X, et al. 2023. J Clin Invest. 133:. PubMed
  5. Read KA, et al. 2023. Nat Commun. 14:1652. PubMed
  6. Corria-Osorio J, et al. 2023. Nat Immunol. 24:869. PubMed
  7. Reyes RM, et al. 2021. Oncoimmunology. 10:2006529. PubMed
  8. Dolgova EV, et al. 2022. Int J Mol Sci. 23:. PubMed
  9. Tsuchiya N, et al. 2020. Cell Reports. 29(1):162-175.e9.. PubMed
  10. Alsaleh G, et al. 2020. Elife. 9: . PubMed
  11. Ren Y, et al. 2022. J Immunother Cancer. 10:. PubMed
  12. Zhang X, et al. 2021. Mol Cancer Res. 19:1076. PubMed
  13. Kim DK, et al. 2022. Nat Commun. 13:6292. PubMed
  14. Haque A, et al. 2020. Exp Neurol. 330:113315. PubMed
  15. Li D, et al. 2020. Immunohorizons. 0.661805556. PubMed
  16. Tao Z, et al. 2022. Cells. 11:. PubMed
  17. Wang F, et al. 2021. Neoplasia. 23:281. PubMed
RRID
AB_2721462 (BioLegend Cat. No. 154303)
AB_2721462 (BioLegend Cat. No. 154304)

Antigen Details

Structure
70 kD Protein that oligomerizes for pore formation.
Distribution

CTL, NK (Cytoplasmic Granules)

Function
Target cell lysis
Cell Type
NK cells, T cells
Biology Area
Immunology
Molecular Family
Cytokines/Chemokines
Antigen References
  1. Podack ER, et al. 1988. Immunol. Rev. 103:203.
  2. Konjar S, et al. 2010. Immunology. 131:257.
  3. Trapani JA, et al. 2002. Nat. Rev. Immunol. 2:735.
  4. Osinska I, et al. 2014. Cent. Eur. J. Immnol. 39:109.
Gene ID
18646 View all products for this Gene ID
UniProt
View information about Perforin on UniProt.org
Go To Top Version: 0    Revision Date: 11.16.2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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