Loading...

APC/Cyanine7 anti-mouse TER-119/Erythroid Cells Antibody

Pricing & Availability
Clone
TER-119 (See other available formats)
Regulatory Status
RUO
Other Names
Ly-76
Isotype
Rat IgG2b, κ
Ave. Rating
Submit a Review
Product Citations
publications
TER-119_APCCyanine7_TER119-Erythroid_Antibody_020119
C57BL/6 bone marrow cells were stained with TER-119 APC/Cyanine7 (filled histogram) or Rat IgG2b, κ APC/Cyanine7 isotype control (open histogram). Data shown was gated on small scatter population.
  • TER-119_APCCyanine7_TER119-Erythroid_Antibody_020119
    C57BL/6 bone marrow cells were stained with TER-119 APC/Cyanine7 (filled histogram) or Rat IgG2b, κ APC/Cyanine7 isotype control (open histogram). Data shown was gated on small scatter population.
Compare all formats See APC/Cyanine7 spectral data
Cat # Size Price Quantity Check Availability Save
116223 25 µg 94 CHF
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

The TER-119 antigen is a 52 kD glycophorin A-associated protein, also known as Ly-76. TER-119 is an erythroid-specific antigen expressed on early proerythroblasts to mature erythrocytes, but not on erythroid colony-forming cells (BFU-E, blast-forming unit erythroid, or CFU-E, colony-forming unit erythroid).

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Day-14 fetal liver cells from a C57BL/6 mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is = 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

The TER-119 antibody is useful for distinguishing erythrocytes and cells in the erythroid lineage. Additional reported applications (for the relevant formats) include: immunoprecipitation1, Western blotting1, complement-mediated cytotoxicity3, and immunohistochemical staining of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections. Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 116253-116258).

Application References

(PubMed link indicates BioLegend citation)
  1. Kina T, et al. 2000. Br. J. Haematol. 109:280. (IP, WB)
  2. Vannucchi AM, et al. 2000. Blood 95:2559.
  3. Maraskovsky E, et al. 1996. J. Exp. Med. 184:1953. (CMCD)
  4. Grisendi S, et al. 2005. Nature 437:147. (FC)
  5. Bourdeau A, et al. 2007. Blood 109:4220.
  6. Chappaz S, et al. 2007. Blood 110:3862. (FC)
  7. Heuser M, et al. 2007. Blood 110:1639. (FC)
  8. Gough SM, et al. 2014. Cancer Discov. 4:564. PubMed
Product Citations
  1. Chung Y, et al. 2014. J Vis Exp. 89: 51660. PubMed
  2. Casellas Roman HL, et al. 2020. Leuk Res. 94:106372. PubMed
  3. Wang D, et al. 2022. Cell Rep. 39:110587. PubMed
  4. Miyao T, et al. 2022. Elife. 11: . PubMed
  5. Tao X, et al. 2022. EMBO J. 41:e110439. PubMed
  6. VanDyke D, et al. 2022. Cell Rep. 41:111478. PubMed
  7. Ishikawa T, et al. 2023. Bio Protoc. 13:e4588. PubMed
  8. Dube CT, et al. 2022. Epigenetics. 17:444. PubMed
  9. Agrafiotis A, et al. 2023. iScience. 26:106055. PubMed
  10. Gawish R, et al. 2022. Elife. 11:. PubMed
  11. Schloss MJ, et al. 2022. Nat Immunol. 23:605. PubMed
  12. Lefebvre MN, et al. 2021. Cell Rep. 37:109956. PubMed
  13. Viny AD, et al. 2019. Cell Stem Cell. 25:682. PubMed
  14. Cheung P, et al. 2020. Cell Stem Cell. 27(4):590-604.e9. PubMed
  15. Chen S, et al. 2022. Cancer Discov. :. PubMed
  16. Waide ML, et al. 2020. Cell Rep. 33:108503. PubMed
  17. Leimkühler NB, et al. 2020. Cell Stem Cell. 28:637. PubMed
  18. Castiglioni A, et al. 2015. PLoS One. 10:128094. PubMed
  19. Lu SX, et al. 2021. Cell. . PubMed
  20. Inoue D, et al. 2021. Nat Genet. 53:707. PubMed
  21. Cordeiro O, et al. 2016. PLoS One. 11: 0151848. PubMed
  22. Yoshimi A, et al. 2019. Nature. 574:273. PubMed
  23. Kunimoto H, et al. 2018. Cancer Cell. 33:44. PubMed
  24. Uchil PD et al. 2018. Cell host & microbe. 25(1):87-100 . PubMed
  25. Hillel–Karniel C, et al. 2020. Cell Reports. 30(3):807-819.e4.. PubMed
  26. Jackson-Jones LH, et al. 2020. Immunity. 52:700. PubMed
  27. Kleppe M et al. 2018. Cancer cell. 33(1):29-43 . PubMed
  28. Thiriot A et al. 2017. BMC biology. 15(1):45 . PubMed
  29. Zhang C, et al. 2020. Cell Rep. 32:108206. PubMed
  30. Urlaub D, et al. 2019. Arthritis Res Ther. 1.067361111. PubMed
  31. Chauveau A, et al. 2020. Immunity. 52:794. PubMed
  32. Xu R, et al. 2018. Nat Med. 24:823. PubMed
  33. Kräutler NJ, et al. 2020. Cell Reports. 30(4):997-1012.e6.. PubMed
  34. Gordon E, et al. 2015. Proc Natl Acad Sci U S A. 112: 13075 - 13080. PubMed
  35. Yang S, et al. 2022. J Exp Med. 219:. PubMed
  36. Cohen M et al. 2018. Cell. 175(4):1031-1044 . PubMed
  37. Goldstein JM et al. 2019. Cell reports. 27(4):1254-1264 . PubMed
  38. Zhang CR, et al. 2022. Blood Cancer Discov. 3:220. PubMed
  39. Mandal RK, et al. 2021. Cell Reports. 35(6):109094. PubMed
  40. Chen R, et al. 2021. Cell Reports. 34(7):108751. PubMed
  41. Ji G, et al. 2019. Bone Joint J. 101-B:108. PubMed
  42. Lee SC et al. 2018. Cancer cell. 34(2):225-241 . PubMed
  43. Duarte D, et al. 2018. Cell Stem Cell. 22:64. PubMed
  44. Celik H, et al. 2018. Cancer Cell. 34:741. PubMed
  45. Onai N, et al. 2020. Int J Mol Sci. :22. PubMed
  46. Tateishi R, et al. 2015. PLoS One. 10: e0141650. PubMed
  47. Kalamakis G, et al. 2019. Cell. 176:1407. PubMed
  48. Pepe‐Mooney BJ et al. 2019. Cell Stem Cell. 25(1):23-38 . PubMed
  49. Zhu YP et al. 2018. Cell reports. 24(9):2329-2341 . PubMed
  50. Yoshida H, et al. 2019. Cell. 176:897. PubMed
  51. Venugopal J, et al. 2020. Sci Rep. 10:16514. PubMed
  52. Mandal RK, et al. 2020. BMC Biol. 83:18. PubMed
  53. Heil J, et al. 2021. Nat Commun. 12:6963. PubMed
  54. Katzmarski N, et al. 2021. Nat Immunol. 22:1382. PubMed
  55. Phansalkar R, et al. 2021. Elife. 10:. PubMed
  56. Beiter RM, et al. 2022. Sci Rep. 12:12921. PubMed
  57. Luo H, et al. 2019. Cell Rep. 26:945. PubMed
  58. Winter C, et al. 2018. Cell Metab. 28:175. PubMed
  59. McKenney AS, et al. 2018. J Clin Invest. 128:789. PubMed
  60. Wang L, et al. 2020. Nat Commun. 0.654166667. PubMed
  61. Xu X, et al. 2011. Am J Physiol Regul Integr Comp Physiol. 300:R1115. PubMed
  62. Fast EM, et al. 2021. Elife. 10:. PubMed
  63. Angueira AR, et al. 2021. Nat Metab. 3:469. PubMed
  64. Gawish R, et al. 2022. Elife. 11:. PubMed
  65. Rossi G, et al. 2022. Sci Rep. 12:13380. PubMed
  66. Li Z, et al. 2021. J Mol Histol. . PubMed
  67. Han Y, et al. 2019. J Clin Invest. 130:26. PubMed
RRID
AB_2137788 (BioLegend Cat. No. 116223)

Antigen Details

Structure
Associated with glycophorin A, 52 kD
Distribution

Early proerythroblast to mature erythrocyte, but not BFU-E and CFU-E

Cell Type
Erythrocytes
Biology Area
Immunology
Antigen References

1. Kina T, et al. 2000. Br. J. Haematol. 109:280.
2. Ikuta K, et al. 1990. Cell 62:863.
3. Osawa M, et al. 1996. Weir's Handbook of Experimental Immunology. Vol. 2 pp. 66.1-66.5.

Gene ID
104231 View all products for this Gene ID
UniProt
View information about TER-119 on UniProt.org

Other Formats

View All TER-119 Reagents Request Custom Conjugation
Description Clone Applications
APC anti-mouse TER-119/Erythroid Cells TER-119 FC
Biotin anti-mouse TER-119/Erythroid Cells TER-119 FC
FITC anti-mouse TER-119/Erythroid Cells TER-119 FC
PE anti-mouse TER-119/Erythroid Cells TER-119 FC
PE/Cyanine5 anti-mouse TER-119/Erythroid Cells TER-119 FC
Purified anti-mouse TER-119/Erythroid Cells TER-119 FC,IHC-F,IHC-P,IP,WB
Alexa Fluor® 488 anti-mouse TER-119/Erythroid Cells TER-119 FC
Alexa Fluor® 647 anti-mouse TER-119/Erythroid Cells TER-119 FC
Alexa Fluor® 700 anti-mouse TER-119/Erythroid Cells TER-119 FC
PE/Cyanine7 anti-mouse TER-119/Erythroid Cells TER-119 FC
APC/Cyanine7 anti-mouse TER-119/Erythroid Cells TER-119 FC
PerCP anti-mouse TER-119/Erythroid Cells TER-119 FC
PerCP/Cyanine5.5 anti-mouse TER-119/Erythroid Cells TER-119 FC
Brilliant Violet 421™ anti-mouse TER-119/Erythroid Cells TER-119 FC
Pacific Blue™ anti-mouse TER-119/Erythroid Cells TER-119 FC
Brilliant Violet 650™ anti-mouse TER-119/Erythroid Cells TER-119 FC
Brilliant Violet 510™ anti-mouse TER-119/Erythroid Cells TER-119 FC
Brilliant Violet 605™ anti-mouse TER-119/Erythroid Cells TER-119 FC
Purified anti-mouse TER-119/Erythroid Cells (Maxpar® Ready) TER-119 FC,CyTOF®
PE/Dazzle™ 594 anti-mouse TER-119/Erythroid Cells TER-119 FC
Brilliant Violet 785™ anti-mouse TER-119/Erythroid Cells TER-119 FC
TotalSeq™-A0122 anti-mouse TER-119/Erythroid Cells TER-119 PG
APC/Fire™ 750 anti-mouse TER-119/Erythroid Cells TER-119 FC
TotalSeq™-B0122 anti-mouse TER-119/Erythroid Cells TER-119 PG
TotalSeq™-C0122 anti-mouse TER-119/Erythroid Cells TER-119 PG
Ultra-LEAF™ Purified anti-mouse TER-119/Erythroid Cells TER-119 FC,IHC-F,IHC-P,IP,WB,CMCD
Spark Blue™ 550 anti-mouse TER-119/Erythroid Cells TER-119 FC
APC/Fire™ 810 anti-mouse TER-119/Erythroid Cells TER-119 FC
Spark NIR™ 685 anti-mouse TER-119/Erythroid Cells Antibody TER-119 FC
Brilliant Violet 711™ anti-mouse TER-119/Erythroid Cells TER-119 FC
Spark Red™ 718 anti-mouse TER-119/Erythroid Cells (Flexi-Fluor™) TER-119 FC
Spark Blue™ 574 anti-mouse TER-119/Erythroid Cells (Flexi-Fluor™) TER-119 FC
Go To Top Version: 3    Revision Date: 02.01.2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account