Human peripheral blood granulocytes were stained with CD66b (clone 6/40c) Brilliant Violet 421™ (filled histogram) or mouse IgG1, κ isotype control (clone MOPC-21) Brilliant Violet 421™ (open histogram).
Human peripheral blood granulocytes were stained with CD66b (clone 6/40c) Brilliant Violet 421™ (filled histogram) or mouse IgG1, κ isotype control (clone MOPC-21) Brilliant Violet 421™ (open histogram).
Human paraffin-embedded spleen tissue slices were prepared with a standard protocol of deparaffinization and rehydration. Antigen retrieval was done with Citrate buffer 1X pH 6.0 at 95°C for 40 minutes. Tissue was washed with PBS/0.05% Tween 20 twice for five minutes and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/mL of anti-human CD66b (clone 6/40c) Brilliant Violet 421™ (blue) at 4°C overnight. Nuclei were counterstained with Helix NP™ Green (green). The image was scanned with a 10X objective and stitched with Metamorph® software.
CD66b is a 95-100 kD glycosylphosphatidylinositol (GPI)-linked protein also known as CD67, CGM6, and NCA-95. CD66b is a member of the immunoglobulin superfamily, carcinoembryonic antigen (CEA)-like subfamily. CD66b, expressed on granulocytes, has been reported to induce activation in neutrophils and to be involved in heterophilic adhesion with CD66c.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. For immunohistochemistry, a concentration range of 5 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.
This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application References
(PubMed link indicates BioLegend citation)
Singer, et al. 2014. PLoS One. 9(4):e94106. PMID: 24743304
Klaile, et al. 2013. Respir Res. 14:85. PMID 23941132
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