- Clone
- W17079A (See other available formats)
- Regulatory Status
- RUO
- Other Names
- glyceraldehyde-3-phosphate dehydrogenase
- Isotype
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
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607903 | 25 µL | 101 CHF | ||||
607904 | 100 µL | 340 CHF |
GAPDH is well known for its glycolytic function of converting D-glyceraldehyde-3-phosphate to 1,3-bisphosphoglycerate. GAPDH is a ubiquitously expressed and has a molecular mass of 36 kD. Though differentially expressed from tissue to tissue, GAPDH is frequently used as a loading control for assays involving mRNA and protein detection. In more recent studies, GAPDH has been shown to be involved in microtubule bundling, prostate cancer progression, programmed neuronal cell death, DNA replication, and DNA repair. Recent work has elucidated roles for GAPDH in apoptosis, gene expression and nuclear transport. GAPDH may also play a role in neurodegenerative pathologies such as Huntington and Alzheimer's diseases.
Product DetailsProduct Details
- Verified Reactivity
- Human, Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Recombinant protein (161-335 a.a.)
- Formulation
- This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
- Application
-
WB - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.2 µg per ml (1:4000 - 1:16000 dilution). For high sensitivity WB membrane imaging systems, the suggested concentration is 0.01 µg per ml (1:50000 dilution). It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
This clone weakly reacts with rat GAPDH.
- Product Citations
-
- RRID
-
AB_2734504 (BioLegend Cat. No. 607903)
AB_2734504 (BioLegend Cat. No. 607904)
Antigen Details
- Structure
- Belongs to the glyceraldehyde-3-phosphate dehydrogenase family, predicted molecular weight 36 kD. The enzyme exists as a tetramer of identical chains.
- Distribution
-
Ubiquitously expressed, locate in cytoplasm and perinuclear region.
- Function
- Belongs to the glyceraldehyde-3-phosphate dehydrogenase family, predicted molecular weight 36 kD. The enzyme exists as a tetramer of identical chains.
- Interaction
- Interacts with TPPP. Interacts with EIF1AD and WARS. Interacts with SUMO4, GLUT4, nPKC-iota and CAMK2.
- Biology Area
- Cell Biology, Western Blot Controls
- Antigen References
-
- Ercolani L, et al. 1988. J. Biol. Chem. 263:15335.
- Meyer-Siegler K, et al. 1991. Proc. Natl. Acad. Sci. USA. 88:8460.
- Hara MR, Snyder SH. 2006. Cell. Mol. Neurobiol. 26:527.
- Zheng L, et al. 2003. Cell. 114:255.
- Wang Q, et al. 2005. FASEB J. 19:869.
- Bae BI, et al. 2006. Proc. Natl. Acad. Sci. USA. 103:3405.
- Gene ID
- 5590 View all products for this Gene ID
- UniProt
- View information about GAPDH on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
Other Formats
View All GAPDH Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-GAPDH | W17079A | WB,ICC |
Direct-Blot™ HRP anti-GAPDH | W17079A | WB |
Alexa Fluor® 488 anti-GAPDH | W17079A | ICC |
Customers Also Purchased
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
-
Purified anti-GAPDH
Whole cell lysates (15 µg protein) from K562, PC3, HeLa, Mol... HeLa cells were fixed with cold methanol for 15 minutes and... -
Direct-Blot™ HRP anti-GAPDH
Western Blot of human lysate using film imaging system. Whol... Western Blot of human lysate using digital imaging system. W... Western Blot of mouse lysate using digital imaging system. W... -
Alexa Fluor® 488 anti-GAPDH
HeLa cells were fixed with cold methanol for 10 minute and ...
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