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Direct-Blot™ HRP anti-RPS6 Phospho (Ser235/Ser236) Antibody

Pricing & Availability
Clone
A17020B (See other available formats)
Regulatory Status
RUO
Other Names
S6, 40S ribosomal protein S6
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
A17020B_DB-HRP_RPS6-Phospho_Antibody_022522
Total cell lysates (15 µg protein) from serum-starved NIH/3T3 cells treated without (-) or with (+) 100 ng/mL Recombinant Mouse PDGF-BB (Cat. No. 558804) for 20 minutes were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to nitrocellulose, and probed with a 1:2000 dilution of Direct-Blot™ HRP anti-RPS6 Phospho (Ser235/Ser236) (clone A17020B). Proteins were visualized by chemiluminescence detection. Equal protein loading was confirmed using purified anti-RPS6 (Cat. No. 643807) and Direct-Blot™ HRP anti-β-actin (Cat. No. 643807). Lane M: molecular weight ladder
  • A17020B_DB-HRP_RPS6-Phospho_Antibody_022522
    Total cell lysates (15 µg protein) from serum-starved NIH/3T3 cells treated without (-) or with (+) 100 ng/mL Recombinant Mouse PDGF-BB (Cat. No. 558804) for 20 minutes were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to nitrocellulose, and probed with a 1:2000 dilution of Direct-Blot™ HRP anti-RPS6 Phospho (Ser235/Ser236) (clone A17020B). Proteins were visualized by chemiluminescence detection. Equal protein loading was confirmed using purified anti-RPS6 (Cat. No. 643807) and Direct-Blot™ HRP anti-β-actin (Cat. No. 643807). Lane M: molecular weight ladder
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608611 25 µL 106 CHF
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608612 100 µL 340 CHF
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Description

Ribosomal protein S6 (RPS6) is a key component of the small 40S ribosomal subunit and is the major substrate of protein kinases in eukaryotic ribosomes. In response to various cellular stimuli such as mitogenic stimulation, insulin, and increased nutrient availability, upstream kinases such as RSK and p70 kinases phosphorylate RPS6 at multiple serine sites. These modifications facilitate the recruitment of the  7-methylguanasine cap complex, thereby promoting the assembly of the translational pre-initiation complex and increased cellular protein synthesis capacity. RPS6 has been shown to be hyperphosphorylated in certain cancers, and phosphorylation is a critical determinant of pancreatic β–cell size and systemic glucose homeostasis function in diabeteic mouse models.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Synthetic peptide from human RPS6 phosphorylated at Serines 235 and 236
Formulation
This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
Preparation
The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested dilution of this reagent is 1:500 - 1:2000. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Due to complete conservation of the immunizing sequence between humans, mouse and rat, this clone is is predicted to react with rat RPS6 phosphorylated at serines 235 and 236.

RRID
AB_2910485 (BioLegend Cat. No. 608611)
AB_2910485 (BioLegend Cat. No. 608612)

Antigen Details

Structure
RPS6 is a 249 amino acid protein with a predicted molecular weight of 28.6 kD.
Distribution

Cytosol, nucleus, ER, ubiquitous expression.

Function
Phosphorylation events at a cluster of serine residues (Ser 235, 236, 240, and 244) at the carboxy-terminus of RPS6 results in increased global translational efficiency. These sites are dephosphorylated during growth arrest.
Interaction
RPS6AK1 and RPS6AK3; other subunits of 40S ribosomal complex.
Biology Area
Cell Biology, Cell Proliferation and Viability, Protein Synthesis, Signal Transduction
Molecular Family
Nuclear Markers, Phospho-Proteins
Antigen References
  1. Jefferies HB, et al. 1997. EMBO J. 16:3693.
  2. Ruvinsky I, et al. 2005. Genes. Dev.19:2199.
  3. Chumacher AM, et al. 2006. Biochemistry. 45:13614
  4. Roux PP, et al. 2007. J. Biol. Chem. 282:14056.
  5. Stevens C, et al. 2009. J. Biol. Chem. 284:334.
  6. Schlafli P, et al. 2011. FEBS J. 278:1757.
Gene ID
6194 View all products for this Gene ID
UniProt
View information about RPS6 Phospho Ser235/Ser236 on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 02.25.2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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