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FITC anti-human CD43 Antibody

Pricing & Availability
Clone
MEM-59 (See other available formats)
Regulatory Status
RUO
Other Names
Leukosialin, leuokocyte sialoglycoprotein, sialophorin, galactoglyprotein
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
MEM-59_FITC_062707
Human peripheral blood lymphocytes stained with MEM-59 FITC
  • MEM-59_FITC_062707
    Human peripheral blood lymphocytes stained with MEM-59 FITC
See FITC spectral data
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315203 25 tests 83 CHF
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315204 100 tests 188 CHF
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Description

CD43 is a 95-135 kD type I transmembrane glycoprotein known as leukosialin, leukocyte sialoglycoprotein, sialophorin, and galactoglyprotein. It is expressed at high levels on the surface of all leukocytes (except on resting B cells) and at low levels on platelets, negative on erythrocytes. The expression of CD43 on T cells and neutrophils is rapidly downregulated by proteolytic shedding after activation with various stimuli. The soluble form of CD43 is present in human serum. It has been reported that CD43 plays a role in anti-adhesive functions, but also mediates adhesion via its interaction with CD54 or some lectins. Similarly, CD43 is implicated in anti-apoptotic and proapoptotic signaling.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human T lymphocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation, Western blotting, immunohistochemistry of formalin-fixed paraffin-embedded sections, and T cell activationSup>1,2.

Application References

(PubMed link indicates BioLegend citation)
  1. Olweus J, et al. 1993. J. Immunol. 151:707.
  2. Alvarado M, et al. 1995. Eur. J. Immunol. 25:1051.
  3. Ye L, et al. 2013. Stem Cell Transl Med. 2:558. PubMed
Product Citations
  1. Ye L, et al. 2013. Stem Cells Trans Med. 2:558. PubMed
  2. Fu Y, et al. 2021. Cell Reports. 36(2):109344. PubMed
RRID
AB_389246 (BioLegend Cat. No. 315203)
AB_389246 (BioLegend Cat. No. 315204)

Antigen Details

Structure
Type I membrane protein, 95-135 kD, soluble form exists in serum
Distribution

Thymocytes, T cells, neutrophils, plasma cells, myelomas

Function
Anti-adhesion, mediate adhesion, regulate T cell activation and neutrophil migration, antiapoptotic and proapoptotic signaling
Interaction
HIPK2
Ligand/Receptor
CD54, Counterreceptor for SN/Siglec-1
Modification
Highly glycosylated
Cell Type
Thymocytes, T cells, Neutrophils, Plasma cells, B cells
Biology Area
Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Leukocyte Typing IV. Knapp W, et al. (Eds.) Oxford University Press (1989)
2. Stephanova I, et al. 1989. Mol. Immunol. 26:153.
3. Alvarado M, et al. 1995. Eur. J. Immunol. 25:1051.
4. Deckert M, et al. 1992. Eur. J. Immunol. 22:2943
5. Cermak L, et al. 2002. J. Biol. Chem. 227:7955.

Gene ID
6693 View all products for this Gene ID
UniProt
View information about CD43 on UniProt.org

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Go To Top Version: 1    Revision Date: 11.30.2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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