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FITC anti-human HLA-DR Antibody

Pricing & Availability
Clone
L243 (See other available formats)
Regulatory Status
RUO
Other Names
Major Histocompatibility Class II, MHC class II
Isotype
Mouse IgG2a, κ
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Product Citations
publications
L243_FITC_072607
Human peripheral blood lymphocytes stained with L243 FITC
  • L243_FITC_072607
    Human peripheral blood lymphocytes stained with L243 FITC
Compare all formats See FITC spectral data
Cat # Size Price Quantity Check Availability Save
307603 25 tests 61 CHF
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307604 100 tests 138 CHF
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307632 100 µg 147 CHF
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Description

HLA-DR is a heterodimeric cell surface glycoprotein comprised of a 36 kD α (heavy) chain and a 27 kD β (light) chain. It is expressed on B cells, activated T cells, monocytes/macrophages, dendritic cells, and other non-professional APCs. In conjunction with the CD3/TCR complex and CD4 molecules, HLA-DR is critical for efficient peptide presentation to CD4+ T cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Cynomolgus, Rhesus
Reported Reactivity
African Green, Baboon, Chimpanzee, Dog, Common Marmoset, Squirrel Monkey, Cotton-topped Tamarin
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
test size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
µg size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration
µg sizes: 0.5 mg/mL
test sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

For flow cytometric staining using the µg size, the suggested use of this reagent is ≤2.0 µg per million cells in 100 µl volume. For flow cytometric staining using test sizes, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Application Notes

The L243 monoclonal antibody reacts with the HLA-DR antigen, a member of MHC class II molecules. It does not cross react with HLA-DP and HLA-DQ. Clone L243 binds a conformational epitope on HLA-DRa which depends on the correct folding of the aß heterodimer.19

Additional reported applications (for the relevant formats) include: immunoprecipitation8, Western blotting8, in vitro blocking of mixed lymphocyte reactions9,10, depeletion of MHC class II cells7, immunohistochemical staining of acetone-fixed frozen sections4,5, and spatial biology (IBEX)21,22. For sensitive functional assays, we recommend using the Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) (Cat. No. 307648, 307665 - 307669).

Application References

(PubMed link indicates BioLegend citation)
  1. Brodsky F. 1984. Immunogenetics 19:179.
  2. Robbins P, et al. 1987. Human Immunol. 18:301.
  3. Stites D, et al. 1986. Clin. Immunol. Immunopathol. 38:161.
  4. Warnke R, et al. 1980. J. Histochem. Cytochem. 28:771. (IHC)
  5. Engleman E, et al. 1981. P. Natl. Acad. Sci. USA 78:1791. (IHC)
  6. Zipf T, et al. 1981. Cancer Res. 41:4786.
  7. Goodier M, et al. 2000. J. Immunol. 165:139. (Depletion)
  8. Esser M, et al. 2001. J. Virol. 75:6173. (IP, WB)
  9. Kalka-Moll WM, et al. 2002. J. Immunol. 169:6149. (Block)
  10. Wang RF, et al. 1999. Science 284:1351. (Block)
  11. Zaba LC, et al. 2007. J. Exp. Med. 204:3183. PubMed
  12. Fujita H, et al. 2009. P. Natl. Acad. Sci. USA 106:21795. PubMed
  13. Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
  14. Goncalves RM, et al. 2010. Infect. Immun. 78:4763. PubMed
  15. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  16. Kim WK, et al. 2006. Am. J. Pathol. 168:822. (FC)
  17. Stein R, et al. 2011. Leuk. Lymphoma 52:273.
  18. Galkowska H, et al. 1996. Vet. Immunol. Immunopathol. 53:329.
  19. Moro M, et al. 2005. BMC Immunol. 6:24.
  20. Lauterbach N, et al. 2014. Mol Immunol. 59:19. PubMed
  21. Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed
  22. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Le Voyer T, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed
  2. Raggi C, et al. 2022. Curr Protoc. 2:e389. PubMed
  3. Scirocchi F, et al. 2022. EBioMedicine. 79:104010. PubMed
  4. Davies LRL, et al. 2022. Sci Transl Med. 14:eabm4065. PubMed
  5. Sharma AL, et al. 2022. iScience. 25:105651. PubMed
  6. Boudreau CM, et al. 2023. Cell Rep Med. 4:100975. PubMed
  7. Guo Q, et al. 2023. Front Immunol. 14:1117545. PubMed
  8. Jiang B, et al. 2023. Sci Signal. 16:eabo3406. PubMed
  9. Ishiuchi N, et al. 2023. Stem Cell Res Ther. 14:121. PubMed
  10. Aabling RR, et al. 2023. Regen Ther. 23:67. PubMed
  11. Smith MH, et al. 2023. Nat Immunol. . PubMed
  12. Lathrop S, et al. 2015. Infect Immun . 83: 2661 - 2671. PubMed
  13. Rochat MA, et al. 2018. Virol J. 15:191. PubMed
  14. Oshchepkova A, et al. 2019. Micromachines (Basel). 0.9375. PubMed
  15. Tu S, et al. 2020. Cellular Signalling. 73:109695. PubMed
  16. Manukyan G, et al. 2020. Auto Immun Highlights. 11:5. PubMed
  17. Wang M, et al. 2022. Immun Inflamm Dis. 10:e626. PubMed
  18. Neuser J, et al. 2017. PLoS One.. 10.1371/journal.pone.0183670. PubMed
  19. Das J, et al. 2021. Med (N Y). 2:1269. PubMed
  20. Santiago K, et al. 2016. Pathog Dis. 74: ftw081. PubMed
  21. Zhou Y, et al. 2017. Front Cell Infect Microbiol. 7:457. PubMed
  22. Yoshimi A, et al. 2019. Nature. 574:273. PubMed
  23. Fang W,et al. 2017. PLoS One. 10.1371/journal.pone.0187088. PubMed
  24. Xia Y, et al. 2019. Gastroenterol Res Pract. 2019:5436961. PubMed
  25. Li J, et al. 2021. Front Cell Dev Biol. 9:737242. PubMed
  26. Li J, et al. 2022. Int J Mol Sci. 23:. PubMed
  27. Muenchhoff M, et al. 2016. Sci Transl Med. 8: 358ra125. PubMed
  28. Wang B, et al. 2017. Stem Cell Res Ther.. 10.1186/s13287-017-0744-6. PubMed
  29. Walter F, et al. 2020. PLoS One. 15:e0239369. PubMed
  30. Yan K, et al. 2022. iScience. 25:104822. PubMed
  31. Ren K, et al. 2022. Brain Behav. 12:e2456. PubMed
  32. Fraccarollo D, et al. 2021. eLife. 0.416666666666667. PubMed
  33. Germundson DL, et al. 2022. Front Allergy. 3:870513. PubMed
  34. Port JR, et al. 2020. J Virol. 94:. PubMed
  35. Jøntvedt Jørgensen M, et al. 2021. Front Cell Infect Microbiol. 11:669623. PubMed
  36. Wang C, et al. 2020. Oncologist. 25:382. PubMed
  37. Chao Y, et al. 2014. J Leukoc Biol. 96:1037. PubMed
  38. Gadd V, et al. 2016. PLoS One. 11: 0157771. PubMed
  39. Khosravi-Maharlooei M, et al. 2021. J Autoimmun. 119:102612. PubMed
  40. Keshtkar S, et al. 2021. Stem Cells Int. 8857457:2020. PubMed
  41. Serrano-Villar S, et al. 2016. PLoS Pathog. 12: 1005381. PubMed
  42. Planès R, et al. 2014. J Virol. 88:6672. PubMed
  43. Iida H, et al. 2008. J Dermatol Sci. 52:31. PubMed
  44. He Z, et al. 2020. J Exp Clin Cancer Res. 39:140. PubMed
  45. Hsieh J, et al. 2013. Nucleic Acids Res. 41:9753. PubMed
  46. Janssen JJE, et al. 2022. Am J Physiol Endocrinol Metab. 322:E141. PubMed
  47. Ran Y, et al. 2022. Stem Cell Res Ther. 13:507. PubMed
  48. Menges D, et al. 2022. Nat Commun. 13:4855. PubMed
  49. Wang X, et al. 2012. J Immunol. 189:3092. PubMed
  50. Collison J, et al. 2015. J Immunol. 195: 1162-1170. PubMed
  51. Sade-Feldman M, et al. 2016. Clin Cancer Res. 22(23):5661-5672. PubMed
  52. Lee PC, et al. 2022. J Clin Invest. 132:. PubMed
  53. Tang X, et al. 2020. Sci Adv. 6:eaaz0374. PubMed
  54. Shan L, et al. 2017. Immunity. 47:766. PubMed
RRID
AB_1089142 (BioLegend Cat. No. 307603)
AB_1089142 (BioLegend Cat. No. 307604)
AB_1089142 (BioLegend Cat. No. 307632)

Antigen Details

Structure
Ig superfamily, MHC class II, heterodimeric transmembrane protein, 36 kD heavy and 27 kD light chain
Distribution

B cells, activated T cells, monocytes/macrophages, dendritic cells, other APCs

Function
Peptide presentation
Ligand/Receptor
CD3/TCR, CD4
Cell Type
Antigen-presenting cells, B cells, Dendritic cells, Macrophages, Monocytes, T cells, Tregs
Biology Area
Immunology, Innate Immunity
Molecular Family
MHC Antigens
Antigen References

1. Levacher M, et al. 1990. Clin. Exp. Immunol. 81:177.
2. Terstappen L, et al. 1990. J. Leukocyte Biol. 48:138.
3. Edwards JA, et al. 1986. J. Immunol. 137:490.
4. van Es A, et al. 1984. Transplantation 37:65.
5. O'Doherty U, et al. 1994. Immunology 82:487.
6. Thomas R, et al. 1994. J. Immunol. 153:4016.
7. Grouard G, et al. 1996. Nature 384:364.

Gene ID
3122 View all products for this Gene ID 3123 View all products for this Gene ID
UniProt
View information about HLA-DR on UniProt.org

Related FAQs

There are no FAQs for this product.

Other Formats

View All HLA-DR Reagents Request Custom Conjugation
Description Clone Applications
APC anti-human HLA-DR L243 FC
FITC anti-human HLA-DR L243 FC
PE anti-human HLA-DR L243 FC
PE/Cyanine5 anti-human HLA-DR L243 FC
Purified anti-human HLA-DR L243 FC,CyTOF®,IP,WB,Block,IHC-F
Biotin anti-human HLA-DR L243 FC
PE/Cyanine7 anti-human HLA-DR L243 FC
APC/Cyanine7 anti-human HLA-DR L243 FC
Alexa Fluor® 488 anti-human HLA-DR L243 FC,SB
Alexa Fluor® 647 anti-human HLA-DR L243 FC
Pacific Blue™ anti-human HLA-DR L243 FC
Alexa Fluor® 700 anti-human HLA-DR L243 FC
PerCP anti-human HLA-DR L243 FC
PerCP/Cyanine5.5 anti-human HLA-DR L243 FC
Brilliant Violet 605™ anti-human HLA-DR L243 FC
Brilliant Violet 421™ anti-human HLA-DR L243 FC
Brilliant Violet 570™ anti-human HLA-DR L243 FC
Brilliant Violet 711™ anti-human HLA-DR L243 FC
Brilliant Violet 785™ anti-human HLA-DR L243 FC
Brilliant Violet 510™ anti-human HLA-DR L243 FC
Ultra-LEAF™ Purified anti-human HLA-DR L243 FC,CyTOF®,IP,WB,Block,IHC-F
Brilliant Violet 650™ anti-human HLA-DR L243 FC
Purified anti-human HLA-DR (Maxpar® Ready) L243 FC,CyTOF®
PE/Dazzle™ 594 anti-human HLA-DR L243 FC
FITC anti-human HLA-DR L243 FC
APC/Fire™ 750 anti-human HLA-DR L243 FC
Pacific Blue™ anti-human HLA-DR L243 FC
APC anti-human HLA-DR L243 FC
PE/Dazzle™ 594 anti-human HLA-DR L243 FC
PE/Cyanine7 anti-human HLA-DR L243 FC
TotalSeq™-A0159 anti-human HLA-DR L243 PG
TotalSeq™-B0159 anti-human HLA-DR L243 PG
TotalSeq™-C0159 anti-human HLA-DR L243 PG
Brilliant Violet 750™ anti-human HLA-DR L243 FC
APC/Fire™ 750 anti-human HLA-DR L243 FC
PerCP/Cyanine5.5 anti-human HLA-DR L243 FC
APC/Fire™ 810 anti-human HLA-DR L243 FC
PE/Fire™ 640 anti-human HLA-DR L243 FC
PE anti-human HLA-DR L243 FC
Spark Violet™ 538 anti-human HLA-DR Antibody L243 FC
KIRAVIA Blue 520™ anti-human HLA-DR L243 FC
TotalSeq™-D0159 anti-human HLA-DR L243 PG
PE/Fire™ 810 anti-human HLA-DR L243 FC
GMP PE/Dazzle™ 594 anti-human HLA-DR L243 FC
Spark Violet™ 423 anti-human HLA-DR L243 FC
PerCP anti-human HLA-DR L243 FC
GMP FITC anti-human HLA-DR L243 FC
GMP APC anti-human HLA-DR L243 FC
GMP PE/Cyanine7 anti-human HLA-DR L243 FC
GMP Pacific Blue™ anti-human HLA-DR L243 FC
GMP APC/Fire™ 750 anti-human HLA-DR L243 FC
Spark Violet™ 500 anti-human HLA-DR L243 FC
GMP PerCP/Cyanine5.5 anti-human HLA-DR L243 FC
GMP PE anti-human HLA-DR L243 FC
Spark UV™ 387 anti-human HLA-DR L243 FC
Spark Blue™ 515 anti-human HLA-DR L243 FC
Spark NIR™ 685 anti-human HLA-DR L243 FC
PerCP/Fire™ 806 anti-human HLA-DR L243 FC
PE/Fire™ 700 anti-human HLA-DR L243 FC
Spark Blue™ 550 anti-human HLA-DR (Flexi-Fluor™) L243 FC
Spark Red™ 718 anti-human HLA-DR (Flexi-Fluor™) L243 FC
PE/Fire™ 744 anti-human HLA-DR L243 FC
Spark PLUS UV395™ anti-human HLA-DR L243 FC
Spark Violet™ 423 anti-human HLA-DR L243 FC
Go To Top Version: 3    Revision Date: 06.17.2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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