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FITC anti-human TCR γ/δ Antibody

Pricing & Availability
Clone
B1 (See other available formats)
Regulatory Status
RUO
Other Names
T cell receptor γ/δ, γ/δ TCR, TCR-γ/δ
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
B1_FITC_1_080112
  • B1_FITC_1_080112
  • B1_FITC_2_080112
Compare all formats See FITC spectral data
Cat # Size Price Quantity Check Availability Save
331207 25 tests 94 CHF
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331208 100 tests 211 CHF
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Description

T cell receptor (TCR) is a heterodimer consisting of an α and a β chain (TCR α/β) or a γ and a δ chain (TCR γ/δ). TCR γ/δ is involved in the recognition of certain bacterial, self-CD1 molecule, and tumor antigens bound to MHC class I. The γ/δ TCR associates with CD3 and is expressed on a subset of T cells found in the thymus, the intestinal epithelium, and the peripheral lymphoid tissues and peritoneum. Most γ/δ T cells are CD4-/CD8-, some are CD8+. T cells expressing the γ/δ TCR have been shown to play a role in oral tolerance, innate immune response for some tumor cells, and autoimmune disease. It has been reported that γ/δ T cells also play a principal role in antigen presentation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Cynomolgus, Rhesus
Reported Reactivity
African Green, Baboon, Pigtailed Macaque
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Clone B1 is also known as clone B1.1.

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections3 and paraffin-embedded sections5, in vitro blocking, and spatial biology (IBEX)8,9. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for highly sensitive assays (Cat. Nos. 331235 and 331236).

Application References

(PubMed link indicates BioLegend citation)
  1. Rodriguez-Gago M, et al. 2001. Transplantation. 72:503.
  2. Lehmann FS, et al. 2002. Am. J. Physiol. Gastrointest. Liver. Physiol. 283:G481. (FC)
  3. Bordignon M, et al. 2008. Mol. Med. Rep. 1:485. (IHC)
  4. Conrad M, et al. 2007. Cytom. Part A 71A:925. (FC)
  5. Pollinger B, et al. 2011. J. Immunol. 186:2602. (IHC)
  6. Correia DV, et al. 2011. Blood. 118:992. (Block)
  7. Laurent AJ, et al. 2014. PLoS One. 9:103683. PubMed
  8. Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed
  9. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Jowett GM, et al. 2022. Cell Rep. 40:111281. PubMed
  2. Corral D, et al. 2022. Cell Rep. 39:110715. PubMed
  3. Zarobkiewicz M, et al. 2022. J Clin Med. 11: . PubMed
  4. McEvoy CM, et al. 2022. Nat Commun. 13:7634. PubMed
  5. Galindo-Albarrán A, et al. 2016. Cell Rep. 17:2151-2160. PubMed
  6. Park JA, et al. 2021. Journal for ImmunoTherapy of Cancer. 9(5):. PubMed
  7. Björklund &, et al. 2016. Nat Immunol. 17:451-460. PubMed
  8. Sundström P, et al. 2015. J Immunol. 195: 3472 - 3481. PubMed
  9. Hansen IS, et al. 2018. Nat Commun. 9:863. PubMed
  10. Wu J, et al. 2021. Front Immunol. 12:601611. PubMed
  11. Yang R, et al. 2020. Cell. 183(7):1826-1847.e31. PubMed
  12. Sefik E, et al. 2021. Nat Biotechnol. . PubMed
  13. Hazenberg MD, et al. 2019. Blood Adv. 2.659722222. PubMed
  14. Golebski K, et al. 2021. Immunity. 54(2):291-307.e7. PubMed
  15. Wang X, et al. 2020. Exp Ther Med. 19:3698. PubMed
  16. Singh A, et al. 2020. Cell Rep. 32:108153. PubMed
  17. Sasawatari S, et al. 2020. J Immunol. 1373:204. PubMed
  18. Okoye AA, et al. 2021. J Clin Invest. 131:. PubMed
RRID
AB_1575108 (BioLegend Cat. No. 331207)
AB_1575108 (BioLegend Cat. No. 331208)

Antigen Details

Structure
Ig superfamily, associates with CD3 complex
Distribution

T subset in thymus, intestinal epithelium, peripheral lymphoid tissues and peritoneum

Function
Antigen recognition
Ligand/Receptor
Some bacterial or tumor antigens bound MHC class I, CD1 molecule
Cell Type
Epithelial cells, T cells
Biology Area
Adaptive Immunity, Immunology
Molecular Family
TCRs
Antigen References

1. Lanier LL, et al. 1987. J. Clin. Immunol. 7:429.
2. Spencer J, et al. 1989. Eur. J. Immunol. 19:1335.
3. Uyemura K, et al. 1991. J. Exp. Med. 174:683.
4. Spada FM, et al. 2000. J. Exp. Med. 191:907.

Gene ID
6964 View all products for this Gene ID 6965 View all products for this Gene ID
UniProt
View information about TCR gamma/delta on UniProt.org
Go To Top Version: 3    Revision Date: 10.15.2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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