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ELISA MAX™ Deluxe Set Mouse IL-10

Pricing & Availability
Regulatory Status
RUO
Other Names
IL-10 ELISA Kit
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Product Citations
publications
IL-10_Mouse_Delx_081712
  • IL-10_Mouse_Delx_081712
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431414 5 plates 305 CHF
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431425 10 Plates 586 CHF
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431426 20 Plates 938 CHF
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Description

Mouse IL-10 was originally described as Cytokine Synthesis Inhibitory Factor (CSIF) due to its ability to inhibit cytokine production by TH1 clones. IL-10 is expressed in activated CD8+ and CD4+ T cells, activated monocytes, mast cells, and Ly-1 B cells. IL-10 shares over 80% sequence homology with the Epstein-Barr virus protein BCRFI. The functions of IL-10 include inhibition of macrophage-mediated cytokine synthesis, suppression of the delayed-type hypersensitivity response, and stimulation of the TH2 cell response, which results in elevated antibody production.


ELISA Development Systems Engineered for Sensitive, Precise and Reliable Serum and Plasma Cytokine Quantitation.

BioLegend's ELISA Max™ Sets provide all the time-savings, convenience and performance of a high-end, commercial ELISA kit for a fraction of the price! BioLegend's ELISA Max™ Sets, in two appealing formats, address the need for sensitivity, accuracy, dependability, and versatility of an ELISA development system, while providing maximum cost effectiveness, value, and ease of use.

Both the ELISA Max™ Set Standard and ELISA Max™ Set Deluxe are specifically engineered for accurate recovery and measurement of analytes in complex fluids, such as serum and plasma (as well as activated cell cultures) without any interference by confounding serum factors, such as heterophilic antibody or rheumatoid factor.

Product Details
Technical Data Sheet (pdf)

Kit Contents

Kit Contents
  • Mouse IL-10 ELISA MAX™ Capture Antibody (200X)
  • Mouse IL-10 ELISA MAX™ Detection Antibody (200X)
  • Mouse IL-10 Standard
  • Avidin-HRP (1000X)
  • Substrate Solution F
  • Coating Buffer B (5X)
  • Assay Diluent A (5X)

Older lots of these products may contain Substrate Solutions A and B instead of Substrate Solution F mentioned on the TDS and the current manual. Please refer to the lot-specific CoA for the list of the reagents included in the lot and follow the ELISA procedure provided there.

Product Details

Verified Reactivity
Mouse
Application

ELISA
 
As a part of BioLegend's efforts to go green, each new lot of the ELISA MAX™ sets will include a summarized version of its manual. Click here for more information.
Application References

(PubMed link indicates BioLegend citation)
  1. Inoue S, et al. 2006. Cancer Research 66:7741.
  2. Lau AH, et al. 2006. J. Leukoc. Biol. 79:941.
  3. Xiong A, et al. 2012. PLoS One. 7:e46230. PubMed.
  4. Xu P, et al. 2013. J. Immunol. 190:5247. PubMed.
Product Citations
  1. Schuster C, et al. 2022. Front Immunol. 13:906499. PubMed
  2. Ahangar P, et al. 2021. Wound Repair Regen. 29:380. PubMed
  3. Li S, et al. 2022. Nature. 610:373. PubMed
  4. Runge EM, et al. 2022. Cells. 11:. PubMed
  5. Gu J, et al. 2023. J Cell Biochem. 124:557. PubMed
  6. Zuo J, et al. 2017. Mol Med Rep. 16:6946. PubMed
  7. Du Q, et al. 2019. Front Microbiol. 10:2050. PubMed
  8. Kalafatakis I, et al. 2021. J Neurosci Res. 99:1474. PubMed
  9. Zhang W, et al. 2022. Front Pharmacol. 13:1031438. PubMed
  10. Xu P, et al. 2013. J Immunol. 190:5247. PubMed
  11. Naji A, et al. 2016. Sci Rep. 6: 26162. PubMed
  12. Wang Y, et al. 2020. Front Immunol. 1.691666667. PubMed
  13. Liu X, et al. 2021. Int J Mol Sci. 22:. PubMed
  14. Ahangar P, et al. 2020. NPJ Regen Med. 24:5. PubMed
  15. Zhang X, et al. 2020. Elife. 9:00. PubMed
  16. Liu R, et al. 2018. Exp Ther Med. 15:3385. PubMed
  17. Hsu CY, et al. 2022. Int J Mol Sci. 23:. PubMed
  18. Chen G, et al. 2021. Sci Adv. 7:eabg5686. PubMed
  19. Li Y, et al. 2021. Sci Transl Med. 13:. PubMed
  20. Wilson JL, et al. 2020. Cell Rep. 30:1542. PubMed
  21. Xiong A, et al. 2012. PLoS One. 7:e46230. PubMed
  22. Qi S, et al. 2022. Transl Stroke Res. :. PubMed
  23. Fan Q, et al. 2021. Bioact Mater. 6:4014. PubMed
  24. Enomoto D, et al. 2017. PLoS One.. 10.1371/journal.pone.0183584. PubMed
  25. Shiozawa S, et al. 2022. iScience. 25:103537. PubMed
  26. Zeng Q, et al. 2022. iScience. 25:105151. PubMed
  27. Kim M, et al. 2018. Immunity. 49:151. PubMed
  28. Stanfield BA, et al. 2021. PLoS One. 16:e0245169. PubMed
  29. Yu L, et al. 2020. Nat Commun. 2.045833333. PubMed
  30. Strudwick XL, et al. 2020. Adv Wound Care (New Rochelle). 9:637. PubMed
  31. de León-Guerrero SD, et al. 2022. Dis Model Mech. . PubMed
  32. Agrahari G, et al. 2021. Front Immunol. 12:628117. PubMed
Sensitivity
16 pg/mL
Standard Range
31.3-2,000 pg/mL
Materials Not Included
  • Microwell plates: 96-well Nunc MaxiSorp™ is recommended.
  • A microplate reader capable of measuring absorbance at 450 nm
  • Adjustable pipettes to measure volumes ranging from 2 µL to 1 mL
  • Deionized (DI) water
  • PBS (Phosphate-Buffered Saline): 8.0 g NaCl, 1.16 g Na2HPO4, 0.2 g KH2PO4, 0.2 g KCl, add deionized water to 1 L; pH to 7.4, 0.2 µm filtered.
  • Wash Buffer (Phosphate-Buffered Saline (PBS) + 0.05% Tween-20, pH=7.4). BioLegend Cat. No. 421601 is recommended.
  • Wash bottle or automated microplate washer
  • Stop Solution (e.g. 2N H2SO4) Biolegend Cat. No. 423001 is recommended
  • Log-Log graph paper or software for data analysis
  • Tubes to prepare standard dilutions
  • Timer
  • Plate Sealer
  • Absorbent paper

Antigen Details

Cell Sources
Activated CD8+ T cells, Th0, Th2 subset of CD4+ T cells, Ly-1+ B cells, monocytes, macrophages, keratinocytes
Cell Type
Tregs
Biology Area
Immunology
Molecular Family
Cytokines/Chemokines
Gene ID
16153 View all products for this Gene ID
UniProt
View information about IL-10 on UniProt.org

Related FAQs

Do the ELISA MAX™ Deluxe Sets come with plates?

No, ELISA MAX™ Deluxe Sets do not come with plates. Coating Buffer and Assay Diluent (for blocking and dilutions) are included in these sets. Plates can be ordered separately (Cat No. 423501), and instructions for coating the plates are in the sets’ product manuals.

Go To Top Version: 7    Revision Date: 01.19.2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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