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Pacific Blue™ anti-human TCR α/β Antibody

Pricing & Availability
Clone
IP26 (See other available formats)
Regulatory Status
RUO
Other Names
α/β TCR, TCR α/β
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
IP26_PB_112409
Human peripheral blood lymphocytes stained with IP26 Pacific Blue™
  • IP26_PB_112409
    Human peripheral blood lymphocytes stained with IP26 Pacific Blue™
Compare all formats See Pacific Blue™ spectral data
Cat # Size Price Quantity Check Availability Save
306715 25 µg 124 CHF
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306716 100 µg 288 CHF
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Description

The IP26 antibody reacts with a monomorphic determinant of the α/β T-cell receptor, which is expressed on greater than 95% of normal peripheral blood CD3+ T cells. The α/β TCR recognizes a peptide bound to MHC leading to T-cell activation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 µg per 106 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Violet Laser (405 nm)
Application Notes

Additional reported applications (for the relevant formats) include: T cell activation. When co-staining with anti-CD3, we recommend using clone UCHT1, since we have confirmed that IP26 does not compete with this clone.  Other anti-CD3 clones may compete out the binding of IP26.  

Application References

(PubMed link indicates BioLegend citation)
  1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York. (FC)
  2. Joseph A, et al. 2008. J. Virol. 82:3078. (FC) PubMed
  3. Pinto JP, et al. 2010. Immunology. 130:217. PubMed
Product Citations
  1. Zhou Y, et al. 2021. Methods Mol Biol. 2388:35. PubMed
  2. Ahmed R et al. 2019. Cell. 177(6):1583-1599 . PubMed
  3. Zhu Y, et al. 2019. Cell Stem Cell. 25:542. PubMed
  4. Li YR, et al. 2021. Cell Rep Med. 2:100449. PubMed
  5. Carrion B, et al. 2021. Neurol Neuroimmunol Neuroinflamm. 8:. PubMed
RRID
AB_1953256 (BioLegend Cat. No. 306715)
AB_1953256 (BioLegend Cat. No. 306716)

Antigen Details

Structure
Ig superfamily, with CD3 forms CD3/TCR complex
Distribution

T cells, thymocytes

Function
Antigen recognition, T cell activation
Ligand/Receptor
Peptide bound to MHC
Cell Type
T cells, Thymocytes, Tregs
Biology Area
Adaptive Immunity, Immunology
Molecular Family
TCRs
Antigen References

1. Marchalonis J, et al. 2002. J. Mol. Recognit. 15:260.

Gene ID
6955 View all products for this Gene ID 6957 View all products for this Gene ID
UniProt
View information about TCR alpha/beta on UniProt.org

Other Formats

View All TCR α/β Reagents Request Custom Conjugation
Description Clone Applications
Biotin anti-human TCR α/β IP26 FC
FITC anti-human TCR α/β IP26 FC
PE anti-human α/β T Cell Receptor IP26 FC
PE/Cyanine5 anti-human TCR α/β IP26 FC
Purified anti-human TCR α/β IP26 FC
PE/Cyanine7 anti-human TCR α/β IP26 FC
Alexa Fluor® 488 anti-human TCR α/β IP26 FC
Alexa Fluor® 647 anti-human TCR α/β IP26 FC
Pacific Blue™ anti-human TCR α/β IP26 FC
APC anti-human TCR α/β IP26 FC
Brilliant Violet 421™ anti-human TCR α/β IP26 FC
PerCP/Cyanine5.5 anti-human TCR α/β IP26 FC
PE/Dazzle™ 594 anti-human TCR α/β IP26 FC
APC/Cyanine7 anti-human TCR α/β IP26 FC
Alexa Fluor® 700 anti-human TCR α/β IP26 FC
Brilliant Violet 510™ anti-human TCR α/β IP26 FC
APC/Fire™ 750 anti-human TCR α/β IP26 FC
Brilliant Violet 605™ anti-human TCR α/β IP26 FC
TotalSeq™-A0224 anti-human TCR α/β IP26 PG
Brilliant Violet 711™ anti-human TCR α/β IP26 FC
Brilliant Violet 785™ anti-human TCR α/β IP26 FC
TotalSeq™-C0224 anti-human TCR α/β IP26 PG
Brilliant Violet 750™ anti-human TCR α/β IP26 FC
TotalSeq™-B0224 anti-human TCR α/β IP26 PG
PE anti-human TCR α/β IP26 FC
APC/Fire™ 810 anti-human TCR α/β IP26 FC
TotalSeq™-D0224 anti-human TCR α/β IP26 PG
GMP PE anti-human TCR α/β IP26 FC
Spark Red™ 718 anti-human TCR α/β (Flexi-Fluor™) IP26 FC
Brilliant Violet 650™ anti-human TCR α/β IP26 FC
FITC anti-human TCR α/β IP26 FC
Go To Top Version: 1    Revision Date: 11.30.2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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