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PE anti-human CD276 (B7-H3) Antibody

Pricing & Availability
Clone
DCN.70 (See other available formats)
Regulatory Status
RUO
Other Names
B7RP2
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
DCNdot70_PE_122408.jpg
Human monocyte-derived dendritic cells stained with DCN.70 PE
  • DCNdot70_PE_122408.jpg
    Human monocyte-derived dendritic cells stained with DCN.70 PE
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331605 25 tests 134 CHF
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331606 100 tests 292 CHF
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Description

B7-H3 is a member of the B7 gene family. It has 88% amino acid sequence identity with mouse B7-H3. It is mostly expressed on professional APCs, including B cells, macrophages, and dendritic cells. Its expression on dendritic cells appears to be up-regulated by LPS. B7-H3 protein inhibits T cell activation and cytokine production. It was reported that monoclonal antibody against B7-H3 enhanced T cell proliferation in vitro and led to exacerbated EAE in vivo. Therefore, B7-H3 may be a negative regulator on T cell activation and function.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Product Citations
  1. Shao L, et al. 2021. Cell Death Discov. 7:145. PubMed
  2. Fang C, et al. 2022. J Pathol. 256:310. PubMed
  3. Watanabe T, et al. 2023. Polymers (Basel). 15:. PubMed
  4. Zeng W, et al. 2017. Front Immunol. 0.806944444. PubMed
  5. Brignole C, et al. 2021. J Exp Clin Cancer Res. 40:180. PubMed
  6. Huang C, et al. 2020. Theranostics. 10:10498. PubMed
  7. Liu T, et al. 2022. Front Immunol. 13:901349. PubMed
  8. Xie J, et al. 2021. FEBS Open Bio. 11:2977. PubMed
  9. Lavoie RR, et al. 2021. Cancers (Basel). 13:. PubMed
  10. Li H, et al. 2020. Front Oncol. 1.477083333. PubMed
RRID
AB_1279197 (BioLegend Cat. No. 331605)
AB_1279197 (BioLegend Cat. No. 331606)

Antigen Details

Structure
About 49.7 KD transmembrane protein characterized by extracellular IgV and IgC domains.
Distribution

B7-H3 is expressed on professional APCs, including B cells, dendritic cells, and macrophages. It is reported to express on a minor fraction of CD4+ and CD8+ cells.

Ligand/Receptor
The receptor has not been identified, but it seems to be expressed on activated T cells.
Bioactivity
B7-H3 is a negative regulator of T cell activation and IL-2 production.
Cell Type
Antigen-presenting cells, B cells
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References

1. Sun M, et al. 2002. J. Immunology. 168:6294
2. Xu J, et al. 2006. Cellular and Molecular Immunology. 3:235

Gene ID
80381 View all products for this Gene ID
UniProt
View information about CD276 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11.30.2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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