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Purified anti-Akt Phospho (Ser473) Antibody

Pricing & Availability
Clone
Poly6490 (See other available formats)
Regulatory Status
RUO
Other Names
Protein kinase B alpha (PRK-BA, PKB), Serine/Threonine specific kinase RAC alpha, Protein kinase Akt
Isotype
Rabbit Polyclonal IgG
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Product Citations
publications
a-poly6490_PURE_AKTphospho(ser473)_Antibody_1_WB_062116
Western blot analysis of NIH3T3 starved overnight (lane 1) cell, NIH3T3 starved overnight and treated with PDGF 100ng/ml for 15 minutes (lane 2) and Jurkat (lane 3) cells using anti-AKT Phospho (Ser473) antibody (Poly6490). Purified anti-β-actin Antibody (Poly6221) was used as loading control.
  • a-poly6490_PURE_AKTphospho(ser473)_Antibody_1_WB_062116
    Western blot analysis of NIH3T3 starved overnight (lane 1) cell, NIH3T3 starved overnight and treated with PDGF 100ng/ml for 15 minutes (lane 2) and Jurkat (lane 3) cells using anti-AKT Phospho (Ser473) antibody (Poly6490). Purified anti-β-actin Antibody (Poly6221) was used as loading control.
  • b-poly6490_PURE_AKT_Antibody_2_ICC_012521
    MDA-MB-231 breast cancer cells were stained with anti-Akt Phospho (Ser473) (clone Poly6490) using 1:50 dilution, followed by Alexa Fluor® 647 anti-rabbit Ig secondary antibody (red) plus DAPI staining for nuclei (blue). Cells were fixed with 4% PFA, permeabilized with 0.1% Triton X-100, blocked with 10% serum, and incubated O/N at 4°C. Images were taken using a TE300 fluorescence microscope under 20x (filter set: EX 647/10x, Dichroic 665LP, EM 700/70x) at exposure 4s. Data provided by John Nolan and Er Liu, La Jolla Bioengineering Institute.
Cat # Size Price Quantity Check Availability Save
649001 50 µL 147 CHF
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649002 200 µL 411 CHF
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Description

Akt (also known as protein kinase B alpha) is a 60 kD serine/threonine specific kinase containing a pleckstrin domain. Akt plays a critical role in controlling survival and apoptosis. This kinase is ubiquitously expressed and translocates to the membrane upon activation. This protein kinase is activated by insulin and various growth and survival factors to function in a wortmannin-sensitive pathway involving PI 3-kinase.  Akt is activated by phospholipid binding and activation loop phosphorylation at Thr308 by PDK1 (4) and by phosphorylation within the carboxy terminus at Ser473. The previously elusive PDK2, responsible for phosphorylation of Akt at Ser473, has been identified as a mammalian target of rapamycin (mTOR) in a rapamycin-insensitive complex with rictor and Sin1. Activated Akt can then phosphorylate a wide range of substrates including transcription factors (e.g. FOXO1), kinases (GSK-3,Raf-1, ASK, Chk1) and other proteins with important signaling roles (e.g. Bad, MDM2).  Akt promotes cell survival by inhibiting apoptosis through phosphorylation and by inactivation of several targets, including Bad, forkhead transcription factor, c-Raf and caspase-9. Akt also plays a critical role in cell growth by directly phosphorylating mTOR in a rapamycin-sensitive complex containing raptor. More importantly, Akt phosphorylates and inactivates tuberin (TSC2), an inhibitor of mTOR within the mTOR-raptor complex. The phosphor-Akt (S473) antibody specifically detects phosphorlated Akt at the Ser473 site.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Polyclonal
Host Species
Rabbit
Immunogen
Modified Synthetic peptide
Formulation
This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 50% glycerol.
Preparation
This antibody was purified by affinity chromatography.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
Upon receipt, store frozen at -20°C.
Application

WB - Quality tested
ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. Western blotting suggested working dilution(s): Use 10 µL antibody per 5 mL antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The optimal concentration should be determined by titration for each individual assay of interest.

This product may contain other non-IgG subtypes.

Additional Product Notes
The 50 µL size can be used for approximately 5 Western blots, and the 200 µL size can be used for approximately 20 Western blots.
Application References

(PubMed link indicates BioLegend citation)
  1. Miyamoto S, et al. 2012. Cancer Res. 72:2609. PubMed
  2. Yin S, et al. 2013. J Biol Chem. 288:26448. PubMed
  3. Duan L, et al. 2014. FEBS Lett. 588:1644. PubMed
Product Citations
  1. Cataldi S, et al. 2021. Cells. 11:. PubMed
  2. Yin S, et al. 2013. J Biol Chem. 288:26448. PubMed
  3. Duan L, et al. 2014. FEBS Lett. 588:1644. PubMed
  4. Miyamoto S, et al. 2012. Cancer Res. 72:2609. PubMed
  5. Penas FN, et al. 2020. Front Immunol. 10:2955. PubMed
  6. Yang Y, et al. 2020. Oncol Rep. 43:1479. PubMed
  7. Saito Y, et al. 2017. Sci Rep. . 10.1038/s41598-017-16443-w. PubMed
  8. Cevey ÁC, et al. 2021. Front Immunol. 12:782891. PubMed
RRID
SCR_001134 (BioLegend Cat. No. 649001)
SCR_001134 (BioLegend Cat. No. 649002)

Antigen Details

Structure
Serine/Threonine family of kinases, pleckstrin domain; 60 kD
Distribution

Ubiquitously expressed, translocates to the membrane upon activation.

Function
Catalytically inactive multimeric complex. Akt is activated by phospholipid binding and activation loop phosphorylation at Thr308 by PDK1 and by phosphorylation within the carboxy terminus at Ser473 by PDK2.
Interaction
Interacts through pleckstrin homology domain with second messengers. Interacts with AKTIP, CDKN1B.
Cell Sources
Phosphorylates substrates in cytoplasm, nucleus.
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Neuroscience, Signal Transduction
Molecular Family
Phospho-Proteins
Antigen References

1. Jacinto E, et al. 2006. Cell 127:125.
2. Inoki K, et al. 2002. Nat. Cell. Biol. 4:648.
3. Franke TF, et al. 1995. Cell 81:727.
4. Brunet A, et al. 1999. Cell 96:857.
5. Yang WL, et al. 2010 Cell Cycle 9:487.

Gene ID
207 View all products for this Gene ID
UniProt
View information about Akt Phospho Ser473 on UniProt.org

Related FAQs

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Go To Top Version: 5    Revision Date: 01.25.2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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