Purified anti-human CD183 CXCR3 Maxpar Ready Antibody anti-CD183

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Clone: G025H7 (See other available formats)
Regulatory Status: RUO
Other Names: CXCR3, G protein-coupled receptor 9 (GPR9), CKR-L2
Isotype: Mouse IgG1, κ
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Product Citations: publications

Human PBMCs stained with 170Er-anti-CD3 (UCHT1) and 156Gd-anti-CD183 (G025H7). Lymphocytes are displayed in the analysis. Data provided by DVS Sciences.

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353733

100 µg

241 CHF

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Description

Human CXCR3, also known as GPR9, is a chemokine receptor that binds CXCL9, CXCL10, and CXCL11. It is a 38 kD seven-pass transmembrane receptor coupled to G-protein. CXCR3 is highly expressed by T cells (Th1), natural killer cells (NK cells), dendritic cells, mast cells, alveolar macrophages, eosinophils, and human airway epithelial cells. CXCR3 is important for effector lymphocyte recruitment into inflamed tissue in various inflammatory and autoimmune diseases, such as chronically inflamed liver, Crohn's disease, rheumatoid arthritis, multiple sclerosis, and inflammatory skin diseases.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Human, Cynomolgus, Rhesus
Reported Reactivity: African Green, Baboon
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human CXCR3 transfectants
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 1.0 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested
CyTOF® - Verified
Recommended Usage: This product is suitable for use with the Maxpar® Metal Labeling Kits. For metal labeling using Maxpar® Ready antibodies, proceed directly to the step to Partially Reduce the Antibody by adding 100 µl of Maxpar® Ready antibody to 100 µl of 4 mM TCEP-R in a 50 kDa filter and continue with the protocol. Always refer to the latest version of Maxpar® User Guide when conjugating Maxpar® Ready antibodies.
Additional Product Notes: Maxpar® is a registered trademark of Standard BioTools Inc.
RRID: AB_2563724 (BioLegend Cat. No. 353733)

Antigen Details

Structure: CXC-chemokine receptor, G protein-coupled receptor, seven-pass transmembrane receptor
Distribution: T cell subset, NK cells, plasmacytoid dendritic cells, GM-CSF activated CD34⁺ hematopoietic progenitors, mast cells, alveolar macrophages, eosinophils, and airway epithelial cells
Function: Essential in T cell recruitment to sites of inflammation
Ligand/Receptor: CXCL9, CXCL10, and CXCL11
Cell Type: Dendritic cells, Eosinophils, Epithelial cells, Hematopoietic stem and progenitors, Macrophages, Mast cells, NK cells, T cells, Tregs
Biology Area: Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family: CD Molecules, Cytokine/Chemokine Receptors, GPCR
Antigen References: 1. Loetscher M, et al. 1996. J. Exp. Med. 184:963.
2. Cole KE, et al. 1998. J. Exp. Med. 187:2009.
3. Aksoy MO, et al. 2006. Am. J. Physiol. Lung Cell Mol. Physiol. 290:L909.
4. Curbishley SM, et al. 2005. Am. J. Pathol. 167:887.
5. Turner JE, et al. 2007. Mini. Rev. Med. Chem. 7:1089.
6. Wenzel J, et al. 2008. J. Invest. Dermatol. 128:67.
Gene ID: 2833 View all products for this Gene ID
UniProt: View information about CD183 on UniProt.org

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Cell Surface Flow Cytometry Staining Protocol

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Related FAQs

Does staining at room temperature or even at 37°C help for checking chemokine receptors expression?

Due to continuous recycling of many chemokine receptors, it may be worthwhile to consider staining at room temperature or at 37°C if the staining at lower temperature (which can potentially reduce receptor turnover) is not optimal.

Can I obtain CyTOF data related to your Maxpar® Ready antibody clones?

We do not test our antibodies by mass cytometry or on a CyTOF machine in-house. The data displayed on our website is provided by Fluidigm^®. Please contact Fluidigm^® directly for additional data and further details.

http://techsupport.fluidigm.com/

Can I use Maxpar® Ready format clones for flow cytometry staining?

We have not tested the Maxpar^® Ready antibodies formulated in solution containing EDTA for flow cytometry staining. While it is likely that this will work in majority of the situations, it is best to use the non-EDTA formulated version of the same clone for flow cytometry testing. The presence of EDTA in some situations might negatively affect staining.

I am having difficulty observing a signal after conjugating a metal tag to your Maxpar® antibody. Please help troubleshoot.

We only supply the antibody and not test that in house. Please contact Fluidigm^® directly for troubleshooting advice: http://techsupport.fluidigm.com/

Is there a difference between buffer formulations related to Maxpar® Ready and purified format antibodies?

The Maxpar^® Ready format antibody clones are formulated in Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA. The regular purified format clones are formulated in solution that does not contain any EDTA. Both formulations are however without any extra carrier proteins.

Other Formats

View All CD183 Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-human CD183 (CXCR3)	G025H7	FC
APC/Cyanine7 anti-human CD183 (CXCR3)	G025H7	FC
FITC anti-human CD183 (CXCR3)	G025H7	FC
PE anti-human CD183 (CXCR3)	G025H7	FC
APC anti-human CD183 (CXCR3)	G025H7	FC
Alexa Fluor® 488 anti-human CD183 (CXCR3)	G025H7	FC
Alexa Fluor® 647 anti-human CD183 (CXCR3)	G025H7	FC
PerCP/Cyanine5.5 anti-human CD183 (CXCR3)	G025H7	FC
Brilliant Violet 421™ anti-human CD183 (CXCR3)	G025H7	FC
PE/Cyanine7 anti-human CD183 (CXCR3)	G025H7	FC
Pacific Blue™ anti-human CD183 (CXCR3)	G025H7	FC
Brilliant Violet 510™ anti-human CD183 (CXCR3)	G025H7	FC
Brilliant Violet 605™ anti-human CD183 (CXCR3)	G025H7	FC
Brilliant Violet 650™ anti-human CD183 (CXCR3)	G025H7	FC
Brilliant Violet 711™ anti-human CD183 (CXCR3)	G025H7	FC
Purified anti-human CD183 (CXCR3) (Maxpar® Ready)	G025H7	FC,CyTOF®
PE/Dazzle™ 594 anti-human CD183 (CXCR3)	G025H7	FC
PerCP anti-human CD183 (CXCR3)	G025H7	FC
Brilliant Violet 785™ anti-human CD183 (CXCR3)	G025H7	FC
Alexa Fluor® 700 anti-human CD183 (CXCR3)	G025H7	FC
Biotin anti-human CD183 (CXCR3)	G025H7	FC
TotalSeq™-A0140 anti-human CD183 (CXCR3)	G025H7	PG
TotalSeq™-C0140 anti-human CD183 (CXCR3)	G025H7	PG
Ultra-LEAF™ Purified anti-human CD183 (CXCR3)	G025H7	Block,FC
TotalSeq™-B0140 anti-human CD183 (CXCR3)	G025H7	PG
APC/Fire™ 750 anti-human CD183 (CXCR3)	G025H7	FC
TotalSeq™-D0140 anti-human CD183 (CXCR3)	G025H7	PG
PE/Fire™ 810 anti-human CD183 (CXCR3) Antibody	G025H7	FC
APC/Fire™ 810 anti-human CD183 (CXCR3) Antibody	G025H7	FC
PE/Cyanine5 anti-human CD183 (CXCR3) Antibody	G025H7	FC
PE/Fire™ 640 anti-human CD183 (CXCR3)	G025H7	FC
PerCP/Fire™ 780 anti-human CD183 (CXCR3)	G025H7	FC
Spark Red™ 718 anti-human CD183 (CXCR3) (Flexi-Fluor™)	G025H7	FC
PE/Fire™ 700 anti-human CD183 (CXCR3)	G025H7	FC

Certificate of Analysis

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Go To Top Version: 2 Revision Date: 07.22.2022

For Research Use Only. Not for diagnostic or therapeutic use.

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.