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Purified anti-human CD1c Antibody

Pricing & Availability
Clone
L161 (See other available formats)
Regulatory Status
RUO
Workshop
V T-CD01.18
Other Names
R7, M241, BDCA-1
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
L161_Pure_082808.jpg
Human peripheral blood lymphocytes stained with purified L161, followed by anti-mouse IgG FITC
  • L161_Pure_082808.jpg
    Human peripheral blood lymphocytes stained with purified L161, followed by anti-mouse IgG FITC
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331501 25 µg 101 CHF
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331502 100 µg 206 CHF
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Description

CD1c, also known as R7 or M241, is a 43 kD member of the five CD1 antigens (CD1a-e) in humans. The CD1 molecules are type I glycoprotein with structural similarities to MHC class I and are non-covalently associated with β2-microglobulin, belonging to the Ig superfamily. CD1c is expressed on cortical thymocytes, Langerhans cells, dendritic cells, and a subset of B cells. It has been reported that CD1c is also expressed on mature T cells in a tightly regulated manner. CD1c is involved in antigen-presentation of glycolipids. It may also act in T cells as an immune regulatory molecule.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
African Green, Baboon, Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC-F, IHC-P - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining on frozen tissue4, 5, formalin-fixed paraffin-embedded immunohistochemical staining6, and spatial biology (IBEX)7,8.

Application References

(PubMed link indicates BioLegend citation)
  1. del C Salamone M, et al. 2001. J Leukoc Biol. 70:567.
  2. de Fraissinette A, et al. 1988. Exp Hematol. 16:764.
  3. Li D, et al. 2012. J Exp Med. 209:109. PubMed
  4. Xu C, et al. 2010. Am J Hematol. 85:539 (IHC-F)
  5. Gerlini G, et al. 2001. J Invest Dermatol. 117:576 (IHC-F)
  6. Poposki J, et al. 2016. Clin Exp Allergy 45:384 (IHC-P) PubMed
  7. Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed
  8. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Dhariwala MO, et al. 2020. Cell Rep Med. 1:100132. PubMed
  2. Leader AM, et al. 2021. Cancer Cell. 39:1594. PubMed
  3. Glass MC, et al. 2022. Cell Rep. 39:110728. PubMed
  4. McCarthy EE, et al. 2022. Cell Rep. 39:110815. PubMed
  5. Poon MML, et al. 2023. Nat Immunol. 24:309. PubMed
  6. Melo-Gonzalez F, et al. 2018. J Biol Chem. 293:8543. PubMed
  7. Alcántara‐Hernández M et al. 2017. Immunity. 47(6):1037-1050 . PubMed
  8. Friebel E, et al. 2020. Cell. 181(7):1626-1642.e20. PubMed
  9. Taft J, et al. 2021. Cell. 184(17):4447-4463.e20. PubMed
  10. Gide TN, et al. 2019. Cancer Cell. 35:238. PubMed
  11. Alcántara-Hernández M, et al. 2021. Nat Protoc. 16:4855. PubMed
  12. Li Y, et al. 2019. Front Immunol. 0.460416667. PubMed
  13. Li D, et al. 2012. J Exp Med. 209:109. PubMed
  14. Liu X, et al. 2022. STAR Protoc. 3:101310. PubMed
  15. Guo T, et al. 2018. J Immunol. 200:500. PubMed
  16. Martin JC, et al. 2020. Cell. 178(6):1493-1508.e20.. PubMed
  17. Borges TJ, et al. 2018. Nat Commun. 9:3482. PubMed
  18. Rybakowska P, et al. 2022. STAR Protoc. 3:101697. PubMed
  19. Farhadian SF, et al. 2022. JCI Insight. 7:. PubMed
  20. Jordan S, et al. 2020. Cell. 178(5):1102-1114.e17.. PubMed
  21. Lee YS, et al. 2021. J Immunother Cancer. 9:. PubMed
  22. Zheng H, et al. 2022. Cell Rep. 39:110864. PubMed
  23. Gherardin NA, et al. 2021. Cancer Immunol Res. 9:612. PubMed
  24. Cillo AR, et al. 2021. Cell Rep Med. 2:100476. PubMed
  25. Kuzmina NA, et al. 2018. Cell Rep. 24:1802. PubMed
  26. Kennedy-Darling J, et al. 2021. Eur J Immunol. 51:1262. PubMed
  27. Sander J et al. 2017. Immunity. 47(6):1051-1066 . PubMed
  28. Chevrier S, et al. 2021. Cell Reports Medicine. 2(1):100166. PubMed
  29. Stensland ZC, et al. 2022. iScience. 25:103626. PubMed
  30. Cytlak U, et al. 2020. Immunity. 53(2):353-370. PubMed
  31. Waugh KA, et al. 2020. Cell Reports. 29(7):1893-1908.e4.. PubMed
  32. Leylek R, et al. 2020. Cell Rep. 32:108180. PubMed
  33. Lavin Y et al. 2017. Cell. 169(4):750-765 . PubMed
RRID
AB_1088995 (BioLegend Cat. No. 331501)
AB_1088995 (BioLegend Cat. No. 331502)

Antigen Details

Structure
43 kD, Ig superfamily, MHC I-like molecule, associates with β2-microglobulin
Distribution

B cell subset, cortical thymocytes, dendritic cells, and Langerhans cells

Function
Presents lipid antigen to CD1c-restricted T cells
Ligand/Receptor
CD1c-restricted TCR
Cell Type
B cells, Dendritic cells, Langerhans cells, Thymocytes
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Fainboim LM and del C. Salamone. 2002. J. Biol. Reg. Homeos. Ag. 16:125.
2. M. del Salamone C, et al. 2001. J. Leukocyte Biol. 70:567.
3. Zola H, et al. Eds. 2007. Leukocyte and Stromal Cell Molecules:The CD Markers. P42.

Gene ID
911 View all products for this Gene ID
UniProt
View information about CD1c on UniProt.org
Go To Top Version: 2    Revision Date: 04.25.2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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