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Purified anti-human CD24 Antibody

Pricing & Availability
Clone
ML5 (See other available formats)
Regulatory Status
RUO
Workshop
V CD24.5
Other Names
Ly-52, Heat Stable Antigen (HSA), Nectadrin, BA-1
Isotype
Mouse IgG2a, κ
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Product Citations
publications
ML5
Human whole blood lymphocytes stained with purified ML5 and detected with anti-mouse IgGs FITC
  • ML5
    Human whole blood lymphocytes stained with purified ML5 and detected with anti-mouse IgGs FITC
  • ML5_IF_072512
    MCF-7 breast cancer cells were stained with anti-CD24 (clone ML5) followed by DyLight™ 649 Goat anti-mouse Ig secondary antibody (red), plus DAPI staining for nuclei (blue). Images were taken under 20x bin4 (Filter set: EX647/10x, Dichroic 665LP, EM 700/70x) at exposure 4s. Data provided by Er Liu and John Nolan, La Jolla Institute for Bioengineering.
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311101 25 µg 59 CHF
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311102 100 µg 114 CHF
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Description

CD24 is a 35-45 kD glycosylphosphatidylinositol (GPI)-linked protein also known as heat stable antigen (HSA), BA-1, Ly-52, and nectadrin. It is expressed on the surface of B cells (but not plasma cells), granulocytes, follicular dendritic cells, and epithelial cells. CD24 may play a role in the regulation of B-cell proliferation and maturation. CD24 crosslinking induces a Ca2+ flux in mature B cells. CD24 has been shown to interact with CD62P (P-selectin).

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
Chimpanzee
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunofluorescence microscopy3.

Application References

(PubMed link indicates BioLegend citation)
  1. Schlossman S, et al. Eds. 1995. Leukocyte Typing V:White Cell Differentiation Antigens. Oxford University Press. New York.
  2. McMichael A, et al. 1987. Leucocyte Typing III. Oxford University Press. New York.
  3. Yang GP, et al. 1999. Nucleic Acids Research 27:1517. (IF)
  4. Kristiansen G, et al. 2003. Clin. Cancer Res. 9:4906. (FC)
Product Citations
  1. Dhariwala MO, et al. 2020. Cell Rep Med. 1:100132. PubMed
  2. McIlwain DR, et al. 2021. Cell Host Microbe. 29:1828. PubMed
  3. McCarthy EE, et al. 2022. Cell Rep. 39:110815. PubMed
  4. Sanmarco LM, et al. 2019. JCI Insight. :4. PubMed
  5. Taft J, et al. 2021. Cell. 184(17):4447-4463.e20. PubMed
  6. Hinohara K, et al. 2018. Cancer Cell. 34:939. PubMed
  7. Clark A, Petty H 2016. Sci Rep. 6:27039. PubMed
  8. Mann ER, et al. 2020. Sci Immunol. :5. PubMed
  9. Kaufmann M, et al. 2021. Med. 2(3):296-312.e8. PubMed
  10. Sigal N, et al. 2022. Methods Mol Biol. 2543:113. PubMed
  11. Gruber CN, et al. 2020. Immunity. 53(3):672-684. PubMed
  12. Jang G, et al. 2022. NPJ Genom Med. 7:63. PubMed
  13. Yang R, et al. 2020. Cell. 183(7):1826-1847.e31. PubMed
  14. Olin A, et al. 2018. Cell. 174:1277. PubMed
  15. Eccles JD, et al. 2020. Cell Rep. 30:351. PubMed
  16. Henrick BM, et al. 2021. Cell. . PubMed
  17. Afzali B, et al. 2017. Nat Immunol. 18:813. PubMed
  18. Jennelle LT, et al. 2021. Clin Transl Gastroenterol. 12:e00353. PubMed
  19. Yang SH, et al. 2019. Artif Cells Nanomed Biotechnol. 47:3021. PubMed
  20. Ding L, et al. 2019. Nat Commun. 10:4182. PubMed
  21. Bouchard G, et al. 2022. Cancer Res. 82:648. PubMed
RRID
AB_314851 (BioLegend Cat. No. 311101)
AB_314851 (BioLegend Cat. No. 311102)

Antigen Details

Structure
GPI-linked glycoprotein, 35-45 kD
Distribution

B cells, granulocytes, epithelial cells

Function
B cell proliferation and differentiation
Ligand/Receptor
CD62P (P-Selectin)
Cell Type
B cells, Epithelial cells, Granulocytes
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Schlossman S, et al. Eds. 1995. Leukocyte Typing V. Oxford University Press. New York.

Gene ID
100133941 View all products for this Gene ID
UniProt
View information about CD24 on UniProt.org
Go To Top Version: 2    Revision Date: 08.09.2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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