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Purified anti-human CD325 (N-Cadherin) Antibody

Pricing & Availability
Clone
8C11 (See other available formats)
Regulatory Status
RUO
Other Names
Cadherin-2, Neural cadherin
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
8C11_PE_101310
Human acute myeloid leukemia cell line (KG1) stained with CD325 (clone 8C11) PE (filled histogram) or mouse IgG1, κ PE isotype control (open histogram)
  • 8C11_PE_101310
    Human acute myeloid leukemia cell line (KG1) stained with CD325 (clone 8C11) PE (filled histogram) or mouse IgG1, κ PE isotype control (open histogram)
  • 8C11_Purified_CD325_Antibody_ICC_042119
    HUVEC cells were fixed with 1% paraformaldehyde (PFA) and then stained with 10 µg/ml of purified anti-human CD325 (N-Cadherin) (clone 8C11) at 4°C overnight, followed by DyLight™ 594 anti-mouse IgG staining at 4°C for 2 hours (red). Nuclei were counterstained with DAPI (blue). The image was captured by 40X objective.
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350802 100 µg 126 CHF
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Description

CD325 (N-cadherin) is a 130 kD, single pass transmembrane protein. Its extracellular region consists of five EC domains and has one cytoplasmic domain. N-cadherin is involved in organogenesis and maintenance of organ architecture by contributing to the sorting of heterogeneous cell types and in the cell adhesion needed to form tissues. N-cadherin is expressed by stem cells, myeloblasts, endothelial cells, and fibroblasts, and also is expressed in neural and muscle tissues and some types of carcinoma cells. CD325 associates with the cytoskeleton trough catenin proteins.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant human N-cadherin extracellular domain
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
ICC - Verified
WB, Block - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µl volume. For immunocytochemistry, a concentration range of 5.0 - 10 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The mAb 8C11 recognizes the amino acids 92–593 of CD325, located between the extracellular cadherin structural domain (EC) 3 and 4. Additional reported applications (for the relevant formats) include: immunofluorescence1,3,6, motility inhibition of N-cadherin-expressing cells2, and Western blot2,4.

Application References

(PubMed link indicates BioLegend citation)
  1. Navarro P, et al. 1998. J. Cell Biol. 140:1475. (IF)
  2. Kim JB. 2000. J. Cell Biol. 151:1193. (Block, WB)
  3. Puch S, et al. 2001. J. Cell. Sci. 114:1567. (IF)
  4. Wahl JK. 3rd, et al. 2003. J. Biol. Chem. 278:17269. (WB)
  5. Wein F, et al. 2010. Stem. Cell Res. 4:129. (FC)
  6. Jaggi M, et al. 2002. Cell. Commun. Adhes. 9:103. (IF)
Product Citations
  1. Kumar M, et al. 2020. Oncotarget. 11:1603. PubMed
  2. Hu J, et al. 2017. Nature. 552:248. PubMed
  3. Li Z, et al. 2020. J Clin Lab Anal. 34:e23155. PubMed
  4. Zhang XZ, et al. 2022. Front Cell Neurosci. 16:888152. PubMed
RRID
AB_10662387 (BioLegend Cat. No. 350802)

Antigen Details

Structure
Member of cadherin family, Single pass transmembrane protein, extracellular region consist of five EC domains, one cytoplasmic domain, 130 kD
Distribution

Stem cells, myeloblasts, endothelial cells, fibroblasts, neural and muscle tissues, some types of carcinoma cells

Function
Embryonic development, maintain tissue architecture, cell sorting and cell adhesion in tissues, promote motility
Interaction
Catenins
Cell Type
Endothelial cells, Fibroblasts, Hematopoietic stem and progenitors, Mesenchymal Stem Cells, Neural Stem Cells
Biology Area
Cell Adhesion, Cell Biology, Cell Motility/Cytoskeleton/Structure, Immunology, Neuroscience, Neuroscience Cell Markers, Stem Cells, Synaptic Biology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Colomiere M, et al. 2009. Brit. J. Cancer 100:134.
2. Yan W, et al. 2010. J. Biol. Chem. 285:14042.
3. Mosnier JF, et al. 2009. Mod. Pathol. 22:182.
4. Gao L, et al. 2010. Stem Cells 28:564.

Gene ID
1000 View all products for this Gene ID
UniProt
View information about CD325 on UniProt.org
Go To Top Version: 3    Revision Date: 04.24.2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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