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Purified anti-human CD86 Antibody

Pricing & Availability
Clone
IT2.2 (See other available formats)
Regulatory Status
RUO
Workshop
VI CD86.8
Other Names
B7-2, B70
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
IT2.2_Purified_CD86_Antibody_FC_050813
Human peripheral blood monocytes were stained with purified CD86 (clone IT2.2) (filled histogram) or purified mouse IgG2b, κ isotype control (open histogram), followed by anti-mouse IgG FITC.
  • IT2.2_Purified_CD86_Antibody_FC_050813
    Human peripheral blood monocytes were stained with purified CD86 (clone IT2.2) (filled histogram) or purified mouse IgG2b, κ isotype control (open histogram), followed by anti-mouse IgG FITC.
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305401 25 µg 77 CHF
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305402 100 µg 185 CHF
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Description

CD86 is an 80 kD immunoglobulin superfamily member also known as B7-2, B70, and Ly-58. CD86 is expressed on activated B and T cells, monocytes/macrophages, dendritic cells, and astrocytes. CD86, along with CD80, is the ligand of CD28 and CD152 (CTLA-4). CD86 is expressed earlier in the immune response than CD80. CD86 has also been shown to be involved in immunoglobulin class-switching and triggering of NK cell-mediated cytotoxicity. CD86 binds to CD28 to transduce costimulatory signals for T cell activation, proliferation, and cytokine production. CD86 can bind to CD152 as well, also known as CTLA-4, to deliver an inhibitory signal to T cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Cynomolgus, Rhesus
Reported Reactivity
African Green, Baboon, Capuchin Monkey, Common Marmoset, Cotton-topped Tamarin, Chimpanzee
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC-F, WB - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per 106 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections6, Western blotting3, and blocking of T cell activation2,4,5. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 305449 & 305450).

Application References

(PubMed link indicates BioLegend citation)
  1. Kishimoto T, et al. Eds. 1997. Leucocyte Typing VI. Garland Publishing Inc. London.
  2. Dieu M. 1998. J. Exp. Med. 188:373. (Block)
  3. Esser M, et al. 2001. J. Virol. 75:6173. (WB)
  4. Jeannin P, et al. 1999. J. Immunol. 162:2044. (Block)
  5. Kapsogeorgou EK, et al. 2001. J. Immunol. 166:3107. (Block)
  6. Geissmann F, et al. 2001. Blood 97:1241. (IHC)
Product Citations
  1. McCarthy EE, et al. 2022. Cell Rep. 39:110815. PubMed
  2. Perera N, et al. 2022. Antiviral Res. 199:105269. PubMed
  3. Melo-Gonzalez F, et al. 2018. J Biol Chem. 293:8543. PubMed
  4. Mask E, et al. 2022. Front Cell Infect Microbiol. 12:880860. PubMed
  5. Laudanski K, et al. 2018. J Transl Med. 0.765972222. PubMed
  6. Nolan E, et al. 2017. Sci Transl Med. 9:eaal4925. PubMed
  7. Martin JC, et al. 2020. Cell. 178(6):1493-1508.e20.. PubMed
  8. Gruber CN, et al. 2020. Cell. 183:982. PubMed
  9. Raffray L, et al. 2020. Immunology. 159:88. PubMed
  10. Azari S, et al. 2021. MBio. 12:e0184921. PubMed
  11. Syrimi E, et al. 2021. iScience. 24:103215. PubMed
  12. Jog NR, et al. 2018. Front Immunol. 9:2198. PubMed
  13. Bruno TC, et al. 2017. Cancer Immunol Res. 0.831944444. PubMed
  14. Soskic B, et al. 2021. Front Immunol. 11:577655. PubMed
  15. Speir M,et al. 2017. Sci Rep.. 10.1038/s41598-017-14690-5. PubMed
  16. Eccles JD, et al. 2020. Cell Rep. 30:351. PubMed
  17. Ehinger E, et al. 2021. Cardiovasc Res. 117:1166. PubMed
  18. Roussel M, et al. 2021. Cell Reports Medicine. 2(6):100291. PubMed
  19. Jayakumar A, et al. 2008. Infect Immun. 76:2138. PubMed
  20. Zimmerman MG, et al. 2018. Cell Host Microbe. 24:731. PubMed
  21. Eriksson E, et al. 2017. Clin Cancer Res. 5.018055556. PubMed
  22. Lavin Y et al. 2017. Cell. 169(4):750-765 . PubMed
RRID
AB_314522 (BioLegend Cat. No. 305401)
AB_314522 (BioLegend Cat. No. 305402)

Antigen Details

Structure
Ig superfamily, single-chain transmembrane glycoprotein, 80 kD
Distribution

Monocytes/macrophages, activated B cells and T cells, dendritic cells

Function
T cell costimulation
Ligand/Receptor
CD28, CD152
Cell Type
B cells, Dendritic cells, Macrophages, Monocytes, T cells, Tregs
Biology Area
Cell Biology, Costimulatory Molecules, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules, Immune Checkpoint Receptors
Antigen References

1. Hathcock K, et al. 1996. Adv. Immunol. 62:131.
2. June C, et al. 1994. Immunol. Today 15:321.

Gene ID
942 View all products for this Gene ID
UniProt
View information about CD86 on UniProt.org
Go To Top Version: 3    Revision Date: 05.10.2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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