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Purified anti-mouse CD206 (MMR) Antibody

Pricing & Availability
Clone
C068C2 (See other available formats)
Regulatory Status
RUO
Other Names
MMR (macrophage mannose receptor), MR (mannose receptor), MRC1
Isotype
Rat IgG2a, κ
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Product Citations
publications
C068C2_Pure_082611
Thioglycollate-elicited BALB/c mouse peritoneal macrophages were intracellularly stained with purified CD206 (clone C068C2) (filled histogram) or rat IgG2a, κ isotype control (open histogram), followed by anti-rat IgG PE.
  • C068C2_Pure_082611
    Thioglycollate-elicited BALB/c mouse peritoneal macrophages were intracellularly stained with purified CD206 (clone C068C2) (filled histogram) or rat IgG2a, κ isotype control (open histogram), followed by anti-rat IgG PE.
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141701 50 µg 118 CHF
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141702 200 µg 265 CHF
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Description

CD206, also known as mannose receptor (MR), is a 175 kD type I membrane protein. It is a pattern recognition receptor (PRR) belonging to the C-type lectin superfamily. MR is expressed on macrophages, dendritic cells, Langerhans cells, and hepatic or lymphatic endothelial cells. MR recognizes a range of microbial carbohydrates bearing mannose, fucose, or N-acetyl glucosamine through its C-type lectin-like carbohydrate recognition domains, sulfated carbohydrate antigens through its cysteine-rich domain, and collagens through its fibronectin type II domain. MR mediates endocytosis and phagocytosis as well as activation of macrophages and antigen presentation. It plays an important role in host defense and provides a link between innate and adaptive immunity. Recently, MR on lymphatic endothelial cells was found to be involved in leukocyte trafficking and a contributor to the metastatic behavior of cancer cells. It suggests that MR may be a potential target in controlling inflammation and cancer metastasis by targeting the lymphatic vasculature.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant mouse CD206 (MMR)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ICFC - Quality tested
FC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone C068C2 recognizes a region similar to clone MR5D3, based on the ability of the clones to block each other. Additional reported applications (for the relevant formats) include: spatial biology (IBEX)4,5.

Application References

(PubMed link indicates BioLegend citation)
  1. Keller J, et al. 2012. Biochem Biophys Res Commun. 417:217. PubMed
  2. Ito H, et al. 2012. J Am Soc Nephrol. 23:1797. PubMed
  3. Yang X, et al. 2015. PNAS. 112:2900. PubMed
  4. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  5. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Blériot C, et al. 2020. Methods Mol Biol. 2164:87. PubMed
  2. Li H, et al. 2022. Cancer Cell. 40:36. PubMed
  3. Scott EP, et al. 2022. Glia. 70:1720. PubMed
  4. Khan KA, et al. 2020. NPJ Breast Cancer. 6:29. PubMed
  5. Loyher PL, et al. 2018. J Exp Med. 215:2536. PubMed
  6. Wang W, et al. 2018. Cancer Cell. 34:757. PubMed
  7. Dasgupta D, et al. 2020. Gastroenterology. 159:1487. PubMed
  8. Zappasodi R, et al. 2021. Nature. 591:652. PubMed
  9. Hilligan KL, et al. 2020. Nat Commun. 11:5637. PubMed
  10. Nolin JD, et al. 2017. JCI Insight. 2:e94929. PubMed
  11. Khan KA, et al. 2020. NPJ Breast Cancer. 6:29. PubMed
  12. Bisht K, et al. 2021. Nat Commun. 12:5289. PubMed
  13. Sun KT, et al. 2018. Front Physiol. 1.06875. PubMed
  14. Hu G, et al. 2021. Nat Commun. 12:773. PubMed
  15. Enders M, et al. 2020. J Immunol. 204:87. PubMed
  16. Tian Z, et al. 2022. Front Integr Neurosci. 15:742377. PubMed
  17. Dong Y, et al. 2020. Front Immunol. 11:29. PubMed
  18. Blriot C, et al. 2021. Immunity. :. PubMed
  19. Gubin MM, et al. 2018. Cell. 175:1014. PubMed
  20. Kim D, et al. 2019. Immune Netw. 19:e32. PubMed
  21. Campesato LF, et al. 2020. Nat Commun. 3.24375. PubMed
  22. Yang X, et al. 2015. Proc Natl Acad Sci U S A. 112:2900. PubMed
  23. Scortegagna M, et al. 2020. Nat Commun. 11:99. PubMed
  24. Wang G, et al. 2019. Front Immunol. 1.380555556. PubMed
  25. Pushalkar S, et al. 2018. Cancer Discov. 0.613194444. PubMed
  26. Kikuchi K, et al. 2018. J Immunol. 201:635. PubMed
RRID
AB_10900233 (BioLegend Cat. No. 141701)
AB_10900233 (BioLegend Cat. No. 141702)

Antigen Details

Structure
Type I transmembrane protein, 175 kD, C-type lectin superfamily
Distribution

Macrophages, dendritic cells, Langerhans cells, liver endothelial cells

Function
Pathogen recognition, endocytosis and phagocytosis, antigen presentation
Ligand/Receptor
Antigen containing mannose, fucose, or an N-acetyl glucosamine
Cell Type
Dendritic cells, Endothelial cells, Langerhans cells, Macrophages
Biology Area
Cell Biology, Immunology, Innate Immunity, Signal Transduction
Molecular Family
CD Molecules
Antigen References

1. Wileman TE, et al. 1986. P. Natl. Acad. Sci. USA 83:2501.
2. Apostolopoulos V, et al. 2001. Curr. Mol. Med. 1:469.
3. Burgdorf S, et al. 2006. J. Immunol. 176:6770.
4. McKenzie EJ, et al. 2007. J. Immunol. 178:4975.

Gene ID
17533 View all products for this Gene ID
UniProt
View information about CD206 on UniProt.org

Related FAQs

Why is mouse CD206 stained intracellularly and not via surface staining?

Typically, mouse CD206 surface level is relatively low under normal conditions and so intracellular staining protocol is required to get better signal.

Go To Top Version: 3    Revision Date: 04.04.2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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