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Purified anti-Synaptophysin Antibody

Pricing & Availability
Clone
SP17 (See other available formats)
Regulatory Status
RUO
Other Names
SYP, Synaptophysin, Major synaptic vesicle protein P38
Isotype
Mouse IgM, κ
Ave. Rating
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Product Citations
publications
SP17_Pure_Synaptophysin_Antibody_1_110918_updated
Western blot of purified anti-Synaptophysin antibody (clone SP17). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate. The blot was incubated with 1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgM. Enhanced chemiluminescence was used as the detection system.
  • SP17_Pure_Synaptophysin_Antibody_1_110918_updated
    Western blot of purified anti-Synaptophysin antibody (clone SP17). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate. The blot was incubated with 1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgM. Enhanced chemiluminescence was used as the detection system.
  • SP17_Pure_Synaptophysin_Antibody_2_110918
    ICC staining of purified anti-Synaptophysin antibody (clone SP17) on U251MG cells. The cells were fixed with 4% PFA, permeabilized with a buffer containing 0.1% Triton X-100 and 0.25% BSA, and blocked with 2% normal goat serum and 0.02% BSA. The cells were then incubated with 10 µg/ml of the primary antibody overnight at 4°C, followed by incubation with 2.5 °g/ml of Alexa Fluor® 647 goat anti-Mouse IgM for one hour at room temperature. Nuclei were counterstained with DAPI, and the slides was mounted with ProLong™ Gold Antifade Mountant. The image was captured with a 40X objective. Scale bar: 50 µm
  • SP17_Pure_Synaptophysin_Antibody_3_110918
    IHC staining of purified anti- anti-Synaptophysin antibody (clone SP17) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 1 µg/ml of the primary antibody overnight at 4°C. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • SP17_Pure_Synaptophysin_Antibody_4_110918
    IHC staining of purified anti- anti-Synaptophysin antibody (clone SP17) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 1 µg/ml of the primary antibody overnight at 4°C. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • SP17_Pure_Synaptophysin_Antibody_5_070620
    IHC staining of purified anti-Synaptophysin (clone SP17) on formalin-fixed paraffin-embedded human brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928602), the tissue was incubated with 5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with 2.5 µg/mL of Alexa Fluor® 647 goat anti-mouse IgG for one hour at room temperature. The slide was mounted with Fluoromount-G™, with DAPI. The image was captured with a 40X objective. Scale Bar: 50 µm
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837103 25 µg 101 CHF
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837104 100 µg 276 CHF
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Description

Synaptophysin, also known as major synaptic vesicle protein P38, is encoded by the SYP gene in humans and is located on the short arm of the X chromosome. Synaptophysin is expressed in neuroendocrine cells as well as all neurons in the CNS that participate in synaptic transmission. Synaptophysin is a synaptic vesicle glycoprotein, and interacts with synaptobrevin. It has been implicated in X-linked mental retardation and can be used as a specific marker for cells of the adrenal medulla and pancreatic islets. As such, it can be used to identify tumors that are derived from those cells, including neuroblastoma, retinoblastoma, medulloblastoma, and others.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This antibody was raised against a crude synaptic preparation from post mortem human brain.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC, IHC-P - Verified
SB - Community verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 10 µg per mL. For immunocytochemistry, a concentration range of 1.0 - 10 μg/mL is recommended. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1.0 - 10 µg/mL for chromogenic staining and 0.5 - 2.0 µg/mL for fluorescent staining is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

SP17 reacts with native, denatured and recombinant synaptic vesicle protein synaptophysin expressed in CHO cells. The antibody is particularly valuable for immunocytochemical studies of the brain due to its stability in formalin sections. 

Additional Product Notes

This product has been verified for IHC-F (Immunohistochemistry - frozen tissue sections) on the NanoString GeoMx® Digital Spatial Profiler. The GeoMx® enables researchers to perform spatial analysis of protein and RNA targets in FFPE and fresh frozen human and mouse samples. For more information about our spatial biology products and the GeoMx® platform, please visit our spatial biology page.

Application References

(PubMed link indicates BioLegend citation)
  1. Frigo DE and McDonnell DP. 2008. Mol. Cancer Ther. 3:659. (WB) PubMed
  2. Wolf AB, et al. 2012. J Alzheimers Dis. 1:217. (ELISA) PubMed
  3. Wolf A, et al. 2012. J Alzheimers Dis. 32:217-232. (ELISA) PubMed
  4. McDonnell D 2008. Mol Cancer Ther. 7:659-669. (WB) PubMed
Product Citations
  1. Yasom S, et al. 2022. FASEB Bioadv. 4:408. PubMed
RRID
AB_2783411 (BioLegend Cat. No. 837103)
AB_2783411 (BioLegend Cat. No. 837104)

Antigen Details

Structure
Synaptophysin is a 313 amino acid protein with an apparent molecular mass of 34 kD.
Distribution

Tissue distribution: Brain, pancreas, adipose and soft tissue, gastrointestinal tract, bone marrow, and immune system.
Cellular Distribution: Plasma and synaptic vesicle membrane.

Function
Synaptophysin plays a role in synaptic vesicle docking and membrane fusion.
Interaction
SRCIN1, also interact with itself to form homohexamer or homotetramer
Biology Area
Cell Biology, Neuroscience, Synaptic Biology
Molecular Family
Presynaptic proteins
Antigen References
  1. Zhang L, et al. 2018. Medicine (Baltimore). 97(29):e11487
  2. Moris, et al. 2018. Anticancer res. 38(2):601
  3. Hami J, et al. 2017. J pediatr Neurosci. 12(3):215
Gene ID
6855 View all products for this Gene ID
UniProt
View information about Synaptophysin on UniProt.org

Related FAQs

If an antibody clone has been previously successfully used in IBEX in one fluorescent format, will other antibody formats work as well?

It’s likely that other fluorophore conjugates to the same antibody clone will also be compatible with IBEX using the same sample fixation procedure. Ultimately a directly conjugated antibody’s utility in fluorescent imaging and IBEX may be specific to the sample and microscope being used in the experiment. Some antibody clone conjugates may perform better than others due to performance differences in non-specific binding, fluorophore brightness, and other biochemical properties unique to that conjugate.

Will antibodies my lab is already using for fluorescent or chromogenic IHC work in IBEX?

Fundamentally, IBEX as a technique that works much in the same way as single antibody panels or single marker IF/IHC. If you’re already successfully using an antibody clone on a sample of interest, it is likely that clone will have utility in IBEX. It is expected some optimization and testing of different antibody fluorophore conjugates will be required to find a suitable format; however, legacy microscopy techniques like chromogenic IHC on fixed or frozen tissue is an excellent place to start looking for useful antibodies.

Are other fluorophores compatible with IBEX?

Over 18 fluorescent formats have been screened for use in IBEX, however, it is likely that other fluorophores are able to be rapidly bleached in IBEX. If a fluorophore format is already suitable for your imaging platform it can be tested for compatibility in IBEX.

The same antibody works in one tissue type but not another. What is happening?

Differences in tissue properties may impact both the ability of an antibody to bind its target specifically and impact the ability of a specific fluorophore conjugate to overcome the background fluorescent signal in a given tissue. Secondary stains, as well as testing multiple fluorescent conjugates of the same clone, may help to troubleshoot challenging targets or tissues. Using a reference control tissue may also give confidence in the specificity of your staining.

How can I be sure the staining I’m seeing in my tissue is real?

In general, best practices for validating an antibody in traditional chromogenic or fluorescent IHC are applicable to IBEX. Please reference the Nature Methods review on antibody based multiplexed imaging for resources on validating antibodies for IBEX.

Go To Top Version: 3    Revision Date: 01.24.2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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