Ultra-LEAF™ Purified anti-mouse/rat/human MCP-1 Antibody

Product Details

Verified Reactivity

Mouse, Rat, Human

Reported Reactivity

Rhesus

Antibody Type

Monoclonal

Host Species

Hamster

Immunogen

CHO-expressed, recombinant mouse MCP-1

Formulation

0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.

Endotoxin Level

Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.

Preparation

The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.

Concentration

The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.

Application

ELISA Capture - Quality tested
ELISPOT Capture, Neut, WB, IHC - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture applications, a concentration range of 5.0 - 10 µg/mL is recommended. To obtain a linear standard curve, serial dilutions of MCP-1 recombinant protein ranging from 2000 to 15 pg/mL are recommended for each ELISA plate.

Application Notes

ELISA or ELISPOT Capture¹: The Ultra-LEAF™ 2H5 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated 4E2/MCP antibody (Cat. No. 506002) as the detecting antibody for the detection of mouse MCP-1. The purified 2H5 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated 5D3-F7 antibody (Cat. No. 502609) as the detecting antibody for the detection of human MCP-1.
Flow Cytometry²: The fluorochrome-labeled 2H5 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify MCP-1 -producing cells within mixed cell populations. For intracellular cytokine staining protocol, please visit www.biolegend.com and click on the support section.
Neutralization^1,4,6: The Ultra-LEAF™ purified antibody (Cat. Nos. 505911 & 505912) is recommended for neutralization.
Additional reported applications (for the relevant formats) include: Western blotting³, immunohistochemistry of paraformaldehyde-fixed, saponin-treated frozen tissue sections⁵, and acetone-fixed frozen tissue sections⁷.

Application References

(PubMed link indicates BioLegend citation)

1. Luo, Y., et al. 1994. J. Immunol. 153:3708.
2. Zhang, Y., et al. 2002. J. Immunol. 168:3088.
3. Luo, Y., et al. 1999 J. Immunol. 163:3985.
4. Morrison, B. E., et al. 2003 J. Clin. Invest. 112:1862.
5. Hancock, W. W., et al. 1997 Transplantation 64:696.
6. Yu, R., et al. 2006 Obesity 14:1353.
7. Taal MW, et al. 2001. Am. J. Physiol. Renal. Physiol. 280:F343.
8. Wan W, et al. 2015. Cardiovasc Res. 106:478. PubMed

Product Citations

1. Feng H, et al. 2020. Cell Death Dis. 1.094444444. PubMed

RRID

AB_2820086 (BioLegend Cat. No. 505914)
AB_2820086 (BioLegend Cat. No. 505916)
AB_2820086 (BioLegend Cat. No. 505915)
AB_2820086 (BioLegend Cat. No. 505911)
AB_2820086 (BioLegend Cat. No. 505912)
AB_2820086 (BioLegend Cat. No. 505913)

Antigen Details

Structure

CC chemokine; dimer; 13.8 kD (Mammalian)

Bioactivity

Chemoattractant; regulates adhesion molecule expression, cytokine production in monocytes; proliferation/activation of CC-chemokine activated killer cells

Cell Sources

Monocytes, vascular endothelial cells, smooth muscle, glomerular mesangial cells

Cell Targets

Monocytes, basophils, T cells, NK cells, immature dendritic cells

Receptors

CCR2; CCR5; CCR10; D6; possibly L-CCR

Biology Area

Cell Biology, Neuroinflammation, Neuroscience

Molecular Family

Cytokines/Chemokines

Antigen References

1. Fitzgerald, K., et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. Bischoff, S., et al. 1992. J. Exp. Med. 175:1271.
3. Charo, I., et al. 1994. P. Natl. Acad. Sci. USA 91:2752.
4. Jiang, Y., et al. 1992. J. Immunol. 148:2423.

Regulation

Upregulated by IL-1, TNF-α, PDGF, TGF-β

Gene ID

20296 View all products for this Gene ID 24770 View all products for this Gene ID 6347 View all products for this Gene ID

UniProt

View information about CCL2 on UniProt.org

Documentation

• Technical Data Sheet (pdf)
• Certificate of Analysis
• Safety Data Sheet

Reviews

Related Protocols

• Active Protocols: Sandwich ELISA - Video
• Sandwich ELISA Protocol

Related Pages & Pathways

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Other Formats

Certificate of Analysis