Cell-Vive™ CD DMSO-Free Cryopreservation Solution, GMP preserves human MSC viability and phenotype, post-cryopreservation, at similar or higher levels to that of a commercially available 10% DMSO solution (Competitor).
After reaching 80% confluency, Human MSCs were detached and stored in liquid nitrogen for two days before thawing. (A) Viability at thaw was determined and (B) cell expansion demonstrated through three passages.
Cell-Vive™ CD DMSO-Free Cryopreservation Solution, GMP preserves the morphology of human MSCs similar to a commercially available 10% DMSO solution (competitor).
Human bone marrow-derived MSCs were frozen at 500,000 cells/mL in (A) Cell-Vive™ CD DMSO-Free Cryopreservation Solution, GMP or (B) a 10% DMSO solution from a competing supplier. Cells were stored in liquid nitrogen for two days, then thawed and plated at 5,000 cells/cm2. Attachment and morphology were observed four days after thawing. Images were taken at 4X magnification.
Cell-Vive™ CD DMSO-Free Cryopreservation Solution, GMP preserves the differentiation capacity of human Mesenchymal Stem Cells.
Human MSCs were stored in liquid nitrogen in the presence of Cell-Vive™ CD DMSO-Free Cryopreservation Solution, GMP for two days. MSCs were thawed and differentiated into (A) osteocytes for nineteen days or (B) adipocytes for twenty-one days with respective differentiation media (left) or cell culture media as a control (right). Osteocytes were stained with Alizarin Red and adipocytes with Oil Red O.
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