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Brilliant Violet 421™ anti-human IFN-γ Antibody

Pricing & Availability
Clone
B27 (See other available formats)
Regulatory Status
RUO
Other Names
Interferon-γ, Immune interferon, Type II interferon, T cell interferon, Macrophage-activating factor (MAF), IFN-g, IFN-gamma
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
B27_BV421_IFN-gamma_Antibody_010419.png
PMA+Ionomycin-stimulated (4 hours) human peripheral blood lymphocytes were fixed and permeabilized then intracellularly stained with CD3 APC (clone UCHT1) and either IFN-γ (clone B27) Brilliant Violet 421™ (left) or mouse IgG1, κ Brilliant Violet 421™ isotype control (right).
  • B27_BV421_IFN-gamma_Antibody_010419.png
    PMA+Ionomycin-stimulated (4 hours) human peripheral blood lymphocytes were fixed and permeabilized then intracellularly stained with CD3 APC (clone UCHT1) and either IFN-γ (clone B27) Brilliant Violet 421™ (left) or mouse IgG1, κ Brilliant Violet 421™ isotype control (right).
Compare all formats See Brilliant Violet 421™ spectral data
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506537 25 tests 159€
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506538 100 tests 322€
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Description

Interferon-γ is a potent multifunctional cytokine which is secreted primarily by activated NK cells and T cells. Originally characterized based on anti-viral activities, IFN-γ also exerts anti-proliferative, immunoregulatory, and proinflammatory activities. IFN-γ can upregulate MHC class I and II antigen expression by antigen-presenting cells. The B27 antibody reacts with the human interferon-γ. The B27 antibody can neutralize the bioactivity of natural or recombinant IFN-γ.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
Chimpanzee, Baboon, Cynomolgus, Rhesus, African Green, Pigtailed Macaque, Sooty Mangabey
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
E. coli-expressed recombinant human IFN-γ
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Flow Cytometry2: The fluorochrome-labeled B27 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IFN-? -producing cells within mixed cell populations. For intracellular cytokine staining protocol, please visit www.biolegend.com and click on the support section.
Neutralization1,3,6,7: The Ultra-LEAF™ Purified antibody (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for neutralization of human IFN-? bioactivity (Cat. No. 506531).

Application References

(PubMed link indicates BioLegend citation)
  1. Favre C, et al. 1989. Molec. Immunol. 26:17. (Neut)
  2. Kaur A, et al. 2002. J Virol. 76:3646.
  3. Abrams J, et al. 1992. Immunol. Rev. 127:5. (Neut)
  4. Andersson U, et al. 1999. Detection and quantification of gene expression. New York:Springer-Verlag.
  5. Rout N, et al. 2010. PLoS One 5:e9787. (FC)
  6. Acosta-Rodriguez EV, et al. 2007. Nat. Immunol. 9:942-9. (Neut)
  7. Gangur V, et al. 1998. FASEB J. 12:705-13. (Neut)
Product Citations
  1. Jiang G, et al. 2020. Aging (Albany NY). 12:11466. PubMed
  2. Mansilla-Soto J, et al. 2022. Nat Med. 28:345. PubMed
  3. Gkika E, et al. 2023. NPJ Precis Oncol. 7:24. PubMed
  4. Yuan Y, et al. 2019. Cancer Med. 8:3811. PubMed
RRID
AB_2801097 (BioLegend Cat. No. 506537)
AB_2801097 (BioLegend Cat. No. 506538)

Antigen Details

Structure
Cytokine; dimer; 20-25 kD (Mammalian)
Bioactivity
Antiviral/antiparasitic activities; inhibits proliferation; enhances MHC class I and II expression on APC
Cell Sources
CD8+ and CD4+ T cells, NK cells
Cell Targets
T cells, B cells, macrophages, NK cells, endothelial cells, fibroblasts
Receptors
IFN-γRα (CDw119) dimerized with IFN-γRβ (AF-1)
Cell Type
Tregs
Biology Area
Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
Cytokines/Chemokines
Antigen References

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. De Maeyer E, et al. 1992. Curr. Opin. Immunol. 4:321.
3. Farrar M, et al. 1993. Annu. Rev. Immunol. 11:571.
4. Gray P, et al. 1987. Lymphokines 13:151.

Regulation
Upregulated by IL-2, FGF-basic, EGF; downregulated by vitamin D3 or DMN; labile at pH2
Gene ID
3458 View all products for this Gene ID
Specificity (DOES NOT SHOW ON TDS):
IFN-gamma
Specificity Alt (DOES NOT SHOW ON TDS):
IFN-γ
App Abbreviation (DOES NOT SHOW ON TDS):
ICFC
UniProt
View information about IFN-gamma on UniProt.org

Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?

It is lot-specific. On average it ranges between 2-4.

Go To Top Version: 2    Revision Date: 12/15/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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