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PE anti-human CD55 Antibody

Pricing & Availability
Clone
JS11 (See other available formats)
Regulatory Status
RUO
Workshop
VI N-L060
Other Names
DAF (Decay Accelerating Factor)
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
JS11
Human peripheral blood lymphocytes stained with JS11 PE, then detected with anti-mouse IgG FITC
  • JS11
    Human peripheral blood lymphocytes stained with JS11 PE, then detected with anti-mouse IgG FITC
  • JS11_PE_CD55_Antibody_042922
    Pre-lysed human blood leukocytes were stained with CD55 (clone JS11) PE (filled histogram) or mouse IgG1, κ PE isotype control (open histogram).

    Data was acquired on the Moxi Flow, exported, and processed using FlowJo software.
Compare all formats See PE spectral data
Cat # Size Price Quantity Check Availability Save
311308 100 tests 215€
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Description

CD55 is a 60-70 kD glycosylphosphatidylinositol (GPI)-anchored single chain glycoprotein also known as decay-accelerating factor (DAF). It is expressed on hematopoietic cells including erythrocytes and many non-hematopoietic cells. CD55 accelerates the dissociation of the components of the C3-convertases (namely C2a from C4b in the C4bC2a complex, a C3-convertase of the classical pathway, and factor Bb from the C3bBb complex, a C3-convertase of the alternative pathway) to protect cells from inappropriate damage caused by autologous complement. CD55 has been reported to reduce the efficiency of NK cell lysis and induce signal transduction in T cells. CD55 has also been shown to interact with CD97 and bind to Coxsackie and Echovirus.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
African Green, Baboon, Cynomolgus
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Kishimoto T, et al. Eds. 1997. Leucocyte Typing VI. Garland Publishing Inc. London.
  2. Peyron P, et al. 2000. J. Immunol. 165:5186
Product Citations
  1. Gunesch JT, et al. 2020. Elife. 9: . PubMed
  2. Qing P, et al. 2020. Exp Physiol. 1708:105. PubMed
  3. DeZern A, et al. 2014. Exp Hematol. 42:857. PubMed
RRID
AB_314865 (BioLegend Cat. No. 311308)

Antigen Details

Structure
GPI-achored regulator of complement activation (RCA), type I membrane glycoprotein, 60-70 kD
Distribution

Hematopoietic cells including erythrocytes and many non-hematopoietic cells

Function
Protects against inappropriate complement activation and cell damage, induces signal transduction, reduces the efficiency of NK cell lysis
Ligand/Receptor
C3b, C4b, CD97, Coxsackie viruses
Biology Area
Cell Biology, Costimulatory Molecules, Immunology, Neuroinflammation, Neuroscience
Molecular Family
CD Molecules
Antigen References

1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
2. Hamann J, et al. 1996. J. Exp. Med. 184:1185.
3. Fujita T, et al. 1987. J. Exp. Med. 166:1221

Gene ID
1604 View all products for this Gene ID
Specificity (DOES NOT SHOW ON TDS):
CD55
Specificity Alt (DOES NOT SHOW ON TDS):
CD55
App Abbreviation (DOES NOT SHOW ON TDS):
FC
UniProt
View information about CD55 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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