PE/Cyanine7 anti-mouse CD279 (PD-1) Antibody

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

PD-1 cDNA followed by PD-1-Ig fusion protein

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine7 under optimal conditions.

Concentration

0.2 mg/mL

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser

Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)

Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue³, in vivo blocking of PD-1 binding to its ligands^2,3, and spatial biology (IBEX)^5,6.

Application References

(PubMed link indicates BioLegend citation)

1. Good-Jacobson KL, et al. 2010. Nat. Immunol. 11:535. (FC) PubMed
2. Lázár-Molnár E, et al. 2008. Proc. Natl. Acad. Sci. USA 105:2658. (Block)
3. Liang SC, et al. 2003. Eur. J. Immunol. 33:2706. (FC, IHC, Block)
4. Tobias J, et al. 2020. Front Immunol. 11:895 (FC, ELISA) PubMed
5. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
6. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed

Product Citations

1. Logan K Smith et al. 2018. Immunity. 48(2):299-312 . PubMed
2. Sandu I, et al. 2020. Nat Commun. 11:4454. PubMed
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5. Dölz M, et al. 2022. iScience. 25:105372. PubMed
6. Edwards SC, et al. 2023. J Exp Med. 220: . PubMed
7. Yeh CH, et al. 2022. Immunity. 55:272. PubMed
8. Zimmerman SM, et al. 2022. Oncogene. 41:4983. PubMed
9. Lao L, et al. 2023. Cancer Immunol Res. 11:320. PubMed
10. Read KA, et al. 2023. Nat Commun. 14:1652. PubMed
11. Pankhurst TE, et al. 2023. Cell Rep. 42:112310. PubMed
12. Zhang YN, et al. 2023. Nat Commun. 14:1985. PubMed
13. Aggen DH, et al. 2020. Clin Cancer Res. . PubMed
14. Wang L, et al. 2019. Cell Rep. 29:1848. PubMed
15. Mogilenko DA, et al. 2020. Immunity. 54(1):99-115.e12. PubMed
16. Shi B, et al. 2018. J Immunol. 200:586. PubMed
17. Wei H, et al. 2021. Malar J. 20:89. PubMed
18. Zhang J, et al. 2021. MedComm (Beijing). 2:256. PubMed
19. Liang S, et al. 2022. Acta Pharm Sin B. 12:2494. PubMed
20. Delacher M, et al. 2021. Immunity. 54(4):702-720.e17. PubMed
21. Browning LM, et al. 2020. Cell Rep. 33:108219. PubMed
22. Choi H et al. 2019. Cell Rep. 27(3):806-819 . PubMed
23. Wu J, et al. 2020. Sci Adv. 6:eaba3458. PubMed
24. Wiede F, et al. 2021. Cancer Discov. Online ahead of print. PubMed
25. Saumyaa, et al. 2016. J Immunol. 196: 3677 - 3685. PubMed
26. Kelsey E Sivick et al. 2018. Cell reports. 25(11):3074-3085 . PubMed
27. Cassidy BR, et al. 2020. J Neuroinflammation. 17:259. PubMed
28. Saumyaa S, et al. 2013. Infect Immun . 81:3426. PubMed
29. Grzelak A, et al. 2018. Int J Mol Sci. 19:. PubMed
30. Yue X, et al. 2019. Nat Commun. 10:2011. PubMed
31. Srivastava S, et al. 2019. Cancer Cell. 35:489. PubMed
32. Cervera–Carrascon V, et al. 2020. Oncoimmunology. 9:1761229. PubMed
33. Watanabe M, et al. 2020. Nat Commun. 4.808333333. PubMed
34. Mirando AC, et al. 2020. Oncoimmunology. 9:1760685. PubMed
35. Kennedy EM, et al. 2022. Nat Commun. 13:5907. PubMed
36. Moore MJ et al. 2018. eLife. 7 pii: e33057. PubMed
37. Ullrich L, et al. 2021. Front Immunol. 12:729607. PubMed
38. Moguche AO et al. 2017. Cell host & microbe. 21(6):695-706 . PubMed
39. Sandu I, et al. 2020. Cell Reports. 32(8):108078. PubMed
40. Blanchard L, et al. 2022. STAR Protoc. 3:101444. PubMed
41. Lou Y, et al. 2021. Int J Mol Sci. 22:. PubMed
42. Dong X, et al. 2022. Front Immunol. 13:896472. PubMed
43. Stephens WZ, et al. 2021. Cell Rep. 37:109916. PubMed
44. Zhang YN, et al. 2021. Sci Adv. 7:eabj3107. PubMed
45. Zeis P, et al. 2020. Immunity. 53:775. PubMed
46. Burton B, et al. 2014. Nat Commun. 5:4741. PubMed
47. Yermanos A, et al. 2020. Front Immunol. 1.143055556. PubMed
48. Skadow M, et al. 2019. J Immunol. 203:370. PubMed
49. Raju S, et al. 2020. Cell Reports. 29(9):2556-2564.e3.. PubMed
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51. Tian D, et al. 2019. Nat Commun. 10:4246. PubMed
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RRID

AB_10689635 (BioLegend Cat. No. 135215)
AB_10689635 (BioLegend Cat. No. 135216)

Antigen Details

Structure

A 50-55 kD glycoprotein belonging to the CD28 family of the Ig superfamily.

Distribution

Induced on splenic T and B lymphocytes, thymocytes, and myeloid cells after stimulation.

Function

Involved in lymphocyte clonal selection and peripheral tolerance, prolonged survival of allografts.

Ligand/Receptor

B7-H1 (PD-L1) and B7-DC (PD-L2)

Cell Type

B cells, T cells

Biology Area

Cancer Biomarkers, Immunology, Inhibitory Molecules

Molecular Family

CD Molecules, Immune Checkpoint Receptors

Antigen References

1. Nishimura H, et al. 2001. Science 291:319
2. Agata Y, et al. 1996. Int. Immunol. 8:765
3. Liang SC, et al. 2003. Eur. J. Immunol. 33:2706
4. Barber DL, et al. 2006. Nature 439:682
5. Keir ME, et al. 2005. J. Immunol. 175:7372
6. Koehn BH. et al. 2008. J Immunol. 181:5313

Gene ID

18566 View all products for this Gene ID

Specificity (DOES NOT SHOW ON TDS):

CD279

Specificity Alt (DOES NOT SHOW ON TDS):

CD279

App Abbreviation (DOES NOT SHOW ON TDS):

FC

UniProt

View information about CD279 on UniProt.org

Documentation

• Technical Data Sheet (pdf)
• Certificate of Analysis
• Safety Data Sheet

Reviews

Related Protocols

• Cell Surface Flow Cytometry Staining Protocol

Related Pages & Pathways

Related FAQs

Other Formats

Certificate of Analysis